Treating a solution of ribonuclease with 2-mercaptoethanol and urea denatures the enzyme. If the 2-mercaptoethanol is removed, followed by the removal of urea, the ability of the enzyme solution to hydrolyse RNA is reduced by about 99%. What is the most plausible explanation for this observation? a.All of the RNase molecules have about 1% of their usual activity, due to changes to the organisation of disulphide bonds b.Random formation of disulphide bonds mean about 1% of the RNase molecules are 100% active whereas 99% have no activity c.Deprotonation of histidine in the active site of the enzyme means that only about 1% of the enzyme molecules are active d.Deprotonation of histidine in the active site of the enzyme means that all of the enzyme molecules have 1% of their usual activity

Introduction 2-mercaptoethanol or also called Beta mercaptoethanol (BE or BME) is a compound (HS -…

Answered: Treating a solution of ribonuclease…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

Treating a solution of ribonuclease with 2-mercaptoethanol and urea denatures the enzyme. If the 2-mercaptoethanol is removed, followed by the removal of urea, the ability of the enzyme solution to hydrolyse RNA is reduced by about 99%. What is the most plausible explanation for this observation?

	a.	All of the RNase molecules have about 1% of their usual activity, due to changes to the organisation of disulphide bonds
	b.	Random formation of disulphide bonds mean about 1% of the RNase molecules are 100% active whereas 99% have no activity
	c.	Deprotonation of histidine in the active site of the enzyme means that only about 1% of the enzyme molecules are active
	d.	Deprotonation of histidine in the active site of the enzyme means that all of the enzyme molecules have 1% of their usual activity

Expert Solution

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions