Translocation during translation requires energy from the hydrolysis of Select one: O a. GTP O b. ATP O c. Elongation factor 1 (EF1) O d. Elongation factor 2 (EF2)
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- Use the bank of terms listed to label all important structures of the diagram of the translation complex below. a. Polypeptide f. Anticodon . 140 b. Amino acids g. MRNA 131 c. tRNA h. 5' 139 d. Ribosome i 3 132 e. IRNA with amino acid attached j. codon Phe Gly 133 135 137 136 138 134 Use the word bank provided to complete the flowchart to show all the major parts of a vertebrate nervous system. central nervous system a. sympathetic nervous system b. brain 141 143 C. autonomic nervous system d. spinal cord e. parasympathetic nervous system peripheral 1. somatic nervous system 144 142 nervous system 145 146 sensory neurons motor neurons ......Formation of the 70S ribosomal initiation complex for prokaryotic translation requires: a.tRNAfMet b.tRNAMet c.GTP d.EF-Tu e.IF-2 f.Mn2+For the following mRNA sequence (reading from left to right) what will be the amino acid sequence following translation? (Use chart provided) AUGCCAGUUGAAUAA A. Pro-Val-Met-Leu-His B. Met-Val-Tyr-Pro C. Met-Pro-Val-Glu D. Correct answer not given E. Met-His-Phe-Ala-Arg
- During the termination of translation, what is the correct polypeptide sequence which will be released by the ribosome? 5' - AUG - UAU - CUC - UUU - 3' (mRNA codon sequence) 3' - UAC - AUA - GAG - AAA - 5' (tRNA anticodon sequence) A.START - Tyr - Leu - Phe B. Tyr - Ile - Glu - Lys C. START - Ile - Glu - Lys D. Ser - Tyr - Gly - CysWhat would be the direct consequence to a cell of loss-of-function of Elongation Factor-Tu (EF-Tu)? You may select multiple answers. a. tRNAs would fail to exit the E site after giving up their amino acid. b. The error rate of translation would increase. c. The ribosome would frequently fail to identify the correct start codon and initiation of translation would become less efficient. d. There would be no pause between the entry of a tRNA into the A site and peptidyl transfer.A poison added to an in vitro translation mixture containing mRNA molecules with the sequence 5'- AUGAAAAAAAAAAAAUAA-3' has the following effect: the only product made is a Met-Lys dipeptide that remains attached to the ribosome. What is the most likely way in which the poison acts to inhibit protein synthesis? It mimics a methionine tRNA. O It inhibits stop proteins from entering the ribosome O It inhibits movement of translocation so it can't empty the P-site O It inhibits the P site from accepting a tRNA to begin with
- 1. Translation a)Explain the role of ribosomes in the process of protein translation.b. Explain the role of tRNA molecules in protein translation.c. Define the following terms:1. codon 2. anticodon. Explain how a codon is used in the process of translation.A synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-Pro- Ile-Ser-Ala. What would be the effect on translation if the following components were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. a. Initiation factor 3 b. Initiation factor 2 c. Elongation factor Tu d. Elongation factor G e. Release factors RF-1, RF-2, and RF-3 f. ATP g. GTPA principle function of 5' and 3' end modifications of eukaryotic mRNA is: a. to ensure that all nucleotides are phosphorylated b. to protect RNA from nucleolytic degradation c. to guide the removal of introns d. to serve as binding sites for translation release factors
- For each of the following initiation factors, how would eukaryoticinitiation of translation be affected if it were missing?A. eIF2B. eIF4C. eIF5For each of the following initiation factors, how would eukaryotic initiation of translation be affected if it were missing? A. eIF 2 B. eIF4 C. eIF5Tomats Provide the three-letter amino acid sequence expected from each of the following mRNA segments. a. 5'GGCAUCUACCGA3' b. 5'GUACGUCGGUUACCAACC3' D. What is the tRNA anticodon if the codon in mRNA is as follows? a. CGC b. ACA c. GAU d. UUC