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To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?
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- To make 200 ml 0.4XSSC (Saline and Sodium Citrate) solution for FISH post-washing, how many ml 20X SSC solution is needed?Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
- The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.
- How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.9. 3 μL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Substrate volume in mL Dilution factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL