To identify a positive control in your lab report, should you list any test tube with positive results? Why or why not? What is a negative control? Which substance is a negative control for each of the tests in this lab exercise?
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- The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.What is the difference between cost per billable test and cost per reportable result. (Give examples referring from the laboratory environment)What is the purpose of the RPR (RAPID PLASMA REAGIN ) Test? Why is a qualitative test performed before a semi-quantitative test? Why is it necessary to rotate the slide/card?
- Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?A nurse reconstituted a vial of 750 mg Cefuroxime Sodium Powder for Injection with 6mL of sterile water for injection. The reconstituted solution was dark amber-colored solution. The package insert states that solution colors range from clear to yellow depending on concentration, diluent, and storage conditions. The nurse was then hesitant to give the patient the solution due to its unusual dark color. Five portions were taken from a batch of cefuroxime sodium. Prior to testing in the instrument, each part was subjected to one of the following conditions: Conditions Specifications Temperature Portion 1: 8°C ± 2°C Portion 2: 30°C ± 2°C Portion 3: 40°C ± 2°C Light Portion 4: Kept in the dark Portion 5: Exposed to direct sunlight 1. Which form of the product – the powder for injection, the reconstituted product, or both – will you subject to the following conditions? Why do you think these forms can best address the problem?A fellow classmate comes to you to ask your opinion about the following result from this SIM test: (Uploaded Picture). a) Provide a full interpretation and analysis of the results this test. b) Your classmate is not fully convinced about the black coloring seen in this result. Please suggest another type of method that would help confirm this result and what explain what the result should show.
- You need to make a 1/750 dilution of each human serum sample prior to testing it in your ELISA. You will need 1 ml of the diluted serum (which already includes extra for Murphy's Law.) Calculate the volume of undiluted human serum you need for your 1 ml test volume. 1.3 microliters O 13 microliters O 0.75 microliters O 7.3 microlitersTo the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.Two 0.9 mL aliquots of a serum are placed in two separate test tubes. These two test tubes are labeled as A and B. To tube-A, 0.1 mL of water is added. To tube-B, 0.1 mL of 200 mg/dL urea is added. Both specimens (A ad B) are analyzed for their urea concentration, and obtained following results. Tube-A= 24 mg/dL Tube-B 43 mg/dL = What is the percent recovery of this experiment? 75% 95% 58% 90%
- Question 1: Suppose you have given 5 tablets weighing 449mg, 450mg, 448mg, 451mg and 453mg, respectively. Calculate 300 mg equivalent sample weight for assay. The product claim is 500 mg Vitamin C is present in each tablet. Question 2: Volume of the standard sodium edetate is 12.6 mL (calculated after the assay of Zinc Sulfate Syrup). Determine the % of potency of the drug considering the claimed dose is 11.56) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?Do you think standard plate counts are very accurate? Why or why not? When doing serial dilutions, why is it necessary to plate more than one dilution? You left a carton of orange juice on your counter for 3 days. When you taste it, it is very bubbly (as though it was carbonated), and it tastes more bitter than usual. (Note: acids taste bitter). What do you think could have happened? (Note: you can’t just say that bacteria grew—you must explain how the growth of the bacteria resulted in the changes in the orange juice.) Based on what you now know about the presence of bacteria on chicken and beef, why is it a good idea to use separate chopping boards for meat and for vegetables? Please help me thank you