The UWC Biotechnolo class undertook a study to determine the microbiological quality of mince-meat samples that were obtained from four local retail outlets. You have performed the following serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferred to 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3) 100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the required medium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the table below: 2 3 4 5 6. Total Viable Count >300 >300 >300 274 28 3 >300 >300 >300 286 29 3 Mold and Yeast >300 >300 129 14 Count >300 >300 127 13 1 Staphylococcal Count >300 168 18 3 10 1 >300 156 16 2 Enterobacteriaceae >300 >300 264 27 3 Count >300 >300 248 25 a. Determine the log colony forming units per gram of product for the different counts. b. If the allowable microbiological standard for the Enterobacteriaceae count is 10 cfu/g, what can you conclude about the overall microbiological standard of the product. [NB - Good or bad will not suffice. You need to provide more explanations for your counts and give recommendations where appropriate]

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 8.1P
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The UWC Biotechnolo
class undertook a study to determine the microbiological quality of
mince-meat samples that were obtained from four local retail outlets. You have performed the
following serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferred
to 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3)
100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the required
medium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the table
below:
1
3
4
6.
Total Viable Count
>300
>300
>300
274
28
3
>300
>300
>300
286
29
3
Mold and Yeast
Count
>300
>300
129
14
2
>300
>300
127
13
1
Staphylococcal
Count
>300
168
18
3
10
1
>300
156
16
2
Enterobacteriaceae
>300
>300
264
27
Count
>300
>300
248
25
2
a.
Determine the log colony forming units per gram of product for the different
counts.
b. If the allowable microbiological standard for the Enterobacteriaceae count is 103
cfu/g, what can you conclude about the overall microbiological standard of the
product. [NB - Good or bad will not suffice. You need to provide more
explanations for your counts and give recommendations where appropriate]
Transcribed Image Text:The UWC Biotechnolo class undertook a study to determine the microbiological quality of mince-meat samples that were obtained from four local retail outlets. You have performed the following serial dilution of the mince samples. Firstly, 25 g of the minced samples was transferred to 225 ml 4 strength Ringers'-solution, followed by two (2) 10-fold dilutions and finally three (3) 100-fold dilutions. These dilutions were then pour-plated out (1000 µl of each) onto the required medium. The colony forming units (cfu) for the Enterobacteriaceae count is listed in the table below: 1 3 4 6. Total Viable Count >300 >300 >300 274 28 3 >300 >300 >300 286 29 3 Mold and Yeast Count >300 >300 129 14 2 >300 >300 127 13 1 Staphylococcal Count >300 168 18 3 10 1 >300 156 16 2 Enterobacteriaceae >300 >300 264 27 Count >300 >300 248 25 2 a. Determine the log colony forming units per gram of product for the different counts. b. If the allowable microbiological standard for the Enterobacteriaceae count is 103 cfu/g, what can you conclude about the overall microbiological standard of the product. [NB - Good or bad will not suffice. You need to provide more explanations for your counts and give recommendations where appropriate]
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