The sample preparation using the EZ:Faast kit involves many steps. Put the following steps in the correct order, first to last. 5 7 4 6 3 2 1 < > > > Extract the derivatised amino acids using a liquid-liquid extraction. The amino acids are in the organic layer. Remove the organic solvent by drying the sample extracts under Nitrogen gas. Derivatise the amino acids using the 'proprietary' derivatising agent. Reconstitute the derivatised amino acids with HPLC mobile phase, prior to assaying them using LC-MS. Elute the amino acids from the SPE media. Use SPE (solid phase extraction) to isolate free amino acids from sample matrix components. Add internal standard.

The sample preparation using the EZ:Faast kit involves many steps. Put the following steps in the correct order, first to last. 5 7 4 6 3 2 1 < > > > Extract the derivatised amino acids using a liquid-liquid extraction. The amino acids are in the organic layer. Remove the organic solvent by drying the sample extracts under Nitrogen gas. Derivatise the amino acids using the 'proprietary' derivatising agent. Reconstitute the derivatised amino acids with HPLC mobile phase, prior to assaying them using LC-MS. Elute the amino acids from the SPE media. Use SPE (solid phase extraction) to isolate free amino acids from sample matrix components. Add internal standard.

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter23: Organic Polymers, Natural And Synthetic

Section: Chapter Questions

Problem 31QAP: On complete hydrolysis, a polypeptide gives two alanine, one leucine, one methionine, one...

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