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The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme Sal I recognizes and cuts this sequence 5-G/TCGAG-3. In both cases, only one of the two DNA strands is shown, / indicates the cut site. A restriction fragment generated by digestion with Sal I is ligated into a plasmid cut at a unique Xho I site. After ligation, can the same fragment be cut back out of the plasmid, and if so, which enzyme(s) can do this? 1) Yes, using Xho1 but not Sall. 2) Yes, using Sal1 but not Xho1. 3) Yes, using either Sall or Xho1. 4) No, neither enzyme nor their combination will work. 5) Yes, using both enzymes - Sal 1 at one end and Xho 1 at the other end.

The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme Sal I recognizes and cuts this sequence 5-G/TCGAG-3. In both cases, only one of the two DNA strands is shown, / indicates the cut site. A restriction fragment generated by digestion with Sal I is ligated into a plasmid cut at a unique Xho I site. After ligation, can the same fragment be cut back out of the plasmid, and if so, which enzyme(s) can do this? 1) Yes, using Xho1 but not Sall. 2) Yes, using Sal1 but not Xho1. 3) Yes, using either Sall or Xho1. 4) No, neither enzyme nor their combination will work. 5) Yes, using both enzymes - Sal 1 at one end and Xho 1 at the other end.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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I don't need explanations Just answer choices please. And Are the selected answers choices for 1-4 correct?
The restriction enzyme Xho I recognizes and outs this sequence: 5-C/TCGAG-3 The restriction enzyme Sal I recognizes and cuts this sequence 5-G/TCGAG-3. In both cases, only one of the two DNA strands is shown, / indicates the cut site. A restriction fragment generated by digestion with Sal I is ligated into a plasmid cut at a unique Xho I site. After ligation, can the same fragment be cut back out of the plasmid, and if so, which enzyme(s) can do this? 1) Yes, using Xho1 but not Sall. 2) Yes, using Sall but not Xho1. 3) Yes, using either Sal1 or Xho1. 4) No, neither enzyme nor their combination will work. 5) Yes, using both enzymes - Sal 1 at one end and Xho 1 at the other end. Question 2 ✔ Saved In 2008, James Watson's genome was sequenced by "whole-genome shotgun" sequencing. This means that: O O 1) Transcribed parts of his genome were isolated as cDNA clones and sequenced. O2) Scientists were kidnapped, brought to Cold Spring Harbor Lab and forced to do the sequencing at gunpoint. 3) Only the protein-coding parts of his genome were sequenced. 4) A physical map of his genome was first constructed by ordering large (10- 100kb) overlapping fragments, each of which was then broken into smaller fragments and sequenced. 5) His genomic DNA was broken into many small (100-1000bp) fragments for sequencing, then overlapping sequences were reassembled by computer. Question 3 Saved A biochemist isolates from a cell an amino-acyl-tRNA (that is, a transfer RNA that has already been joined to an amino acid) with the anticodon 5'AUG3', and changes its anticodon to 5'AUA3. If this altered amino-acyl-tRNA is used in ribosomal translation of a protein, what will the most likely result be? O1) No change to the protein, because of the redundancy allowed by "wobble" pairing. O2) Tyrosine (Tyr) will be inserted at a position in the protein where histidine (His) would normally be found. 3) Histidine (His) will be inserted at a position in the protein where tyrosine (Tyr) would normally be found. 4) The protein will begin with isoleucine (Ile) instead of methionine (Met). 5) The mutant amino-acyl-tRNA will cause frameshift. Question 4 ✔ Saved If 20 different amino acids are used in protein synthesis, how many different pentapeptide (5-amino acid-long) sequences can occur in natural proteins?? 1) 5^20 2) 100 Ⓒ3) 20^5 4) 20x19x18x17x16 5) It depends on how many codons encode each amino acid.
Transcribed Image Text:The restriction enzyme Xho I recognizes and outs this sequence: 5-C/TCGAG-3 The restriction enzyme Sal I recognizes and cuts this sequence 5-G/TCGAG-3. In both cases, only one of the two DNA strands is shown, / indicates the cut site. A restriction fragment generated by digestion with Sal I is ligated into a plasmid cut at a unique Xho I site. After ligation, can the same fragment be cut back out of the plasmid, and if so, which enzyme(s) can do this? 1) Yes, using Xho1 but not Sall. 2) Yes, using Sall but not Xho1. 3) Yes, using either Sal1 or Xho1. 4) No, neither enzyme nor their combination will work. 5) Yes, using both enzymes - Sal 1 at one end and Xho 1 at the other end. Question 2 ✔ Saved In 2008, James Watson's genome was sequenced by "whole-genome shotgun" sequencing. This means that: O O 1) Transcribed parts of his genome were isolated as cDNA clones and sequenced. O2) Scientists were kidnapped, brought to Cold Spring Harbor Lab and forced to do the sequencing at gunpoint. 3) Only the protein-coding parts of his genome were sequenced. 4) A physical map of his genome was first constructed by ordering large (10- 100kb) overlapping fragments, each of which was then broken into smaller fragments and sequenced. 5) His genomic DNA was broken into many small (100-1000bp) fragments for sequencing, then overlapping sequences were reassembled by computer. Question 3 Saved A biochemist isolates from a cell an amino-acyl-tRNA (that is, a transfer RNA that has already been joined to an amino acid) with the anticodon 5'AUG3', and changes its anticodon to 5'AUA3. If this altered amino-acyl-tRNA is used in ribosomal translation of a protein, what will the most likely result be? O1) No change to the protein, because of the redundancy allowed by "wobble" pairing. O2) Tyrosine (Tyr) will be inserted at a position in the protein where histidine (His) would normally be found. 3) Histidine (His) will be inserted at a position in the protein where tyrosine (Tyr) would normally be found. 4) The protein will begin with isoleucine (Ile) instead of methionine (Met). 5) The mutant amino-acyl-tRNA will cause frameshift. Question 4 ✔ Saved If 20 different amino acids are used in protein synthesis, how many different pentapeptide (5-amino acid-long) sequences can occur in natural proteins?? 1) 5^20 2) 100 Ⓒ3) 20^5 4) 20x19x18x17x16 5) It depends on how many codons encode each amino acid.
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