5. The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data? The activity of the GAPDHenzyme was assayed in thepresence of a constantamount of glyceraldehyde-3-phosphate and anincreasing amount ofNAD. The activity of thecontrol (that is, the enzymewith no other metabolites)was compared to theactivity of the enzyme inthe presence of variousmetabolites. The results areshown in the figure to theright.Units/mg proteinR8910-NADP+Hill coeficient = 1Hill coefficient = 2NAD], mM15Control -- AMP-X-Glucose-1-P20

Enzyme are proteins molecules that catalyse the biochemical reactions. They are very crucial for…

Answered: The researchers did not study the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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5. The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data?

The activity of the GAPDH
enzyme was assayed in the
presence of a constant
amount of glyceraldehyde-
3-phosphate and an
increasing amount of
NAD. The activity of the
control (that is, the enzyme
with no other metabolites)
was compared to the
activity of the enzyme in
the presence of various
metabolites. The results are
shown in the figure to the
right.
Units/mg protein
R
8
9
10
-NADP+
Hill coeficient = 1
Hill coefficient = 2
NAD], mM
15
Control -- AMP-X-Glucose-1-P
20

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wouldn't ADP, a reactant of the other (phosphorylating) pathway, tend to inhibit the competing non-phosphorylating pathway (in order to get more ATP made)? And the reverse for ATP (whose presence indicates that pathway may not be needed any more)?

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