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- I missed a couple of my labs due to covid and am struggling to teach myself how to do serial dilution, dilution, dilution factors, and colony forming units per ml of original stock. I thought I was understanding everything until it was time for me to take the practice quiz and I realized that I understood nothing.Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful. How would you prepare a series of dilutions to get a final dilution of 10–10? Outline each step.Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?
- what is means cross-contamination in animal cell culture? *The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
- You've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.You are concerned that the level of lead in drinking water has reached potentially harmful levels in certain regions of the country. How would you go about testing what concentrations of lead would be safe for consumption? Include at least one approach from the following 3 categories: in vitro assay, cell-based test, and, toxicogenomic approach.There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2
- You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of fecal bacteria in the water. The 1 mL sample is diluted into a first bottle with 99 mL of sterile saline. One mL from the first bottle is transferred into a second bottle of saline containing 9 mL. Finally, 1 mL from the second bottle is transferred to a third bottle with 9 mL of saline. You create two spread plates from the third bottle: plating 0.1 mL results in 106 colonies, while plating 1 mL results in 562 colonies. How many fecal bacteria per mL are in the drinking water? This is a homework assignment, and I am struggling to do this correctly. Thanks for your help.Which of the following is NOT a function of the loading buffer used in SDS-PAGE? A) It contains a dye, letting you track the progression of electrophoresis samples B) It keeps the sample from floating away out of the gel well C) It measures the protein concentration of your samples D) It denatures proteins to turn them into linear chains of amino acidsCentrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and Immunoprecipitation