The graph below shows the O₂-binding curves for myoglobin (Mb) and hemoglobin (Hb). a. 100 80 60 40 20 0 0 20 40 60 80 100 pO₂ (torr) a. Use the graph to determine the Kd of myoglobin for O₂ (show your approach). Write that in the provided space. b. What is the difference between myoglobin and hemoglobin that cause the O2- binding curves to so be different? Answer in the provided space. YO?
Q: The following speaks true of metabolism except. 1. Property consistent to all organisms ranging…
A: Metabolism involves the processes of both anabolism and catabolism.
Q: 1. It is used to detect the presence of free amino groups, thus, differentiating primary from…
A: Amino acids are classified as natural compounds that are composed of the amine as well as…
Q: External ophthalmoplegia is associated with which deficiency? Group of answer choices Fumarase…
A: External ophthalmoplegia is a disorder that is caused due to mutations in mitochondrial DNA and is…
Q: Give the positive result for the test of pyrimidines.
A: The nitrogenous bases of nucleic acids are classified into two. They are, the purines and…
Q: 4. Use the cartoon protein below to answer the following questions. Assume the rounder subunits are…
A: Proteins are polymers of amino acids linked by peptide/amide bond (Covalent). Primary Structure of…
Q: Dissipation of the proton gradient is required for Formation of ATP from ADP+Pi…
A: As during electron transport chain, the proton gradient created by proton pumping is utilised to…
Q: hich of the following statements best explains the positive result of the acid hydrolysate of…
A: Answer for the following question are :
Q: arriers, kindly give their alternative names Electron Transport Chain Intermembrane space brane…
A: 1- NADH dehydrogenase - have 40 polynucleotide chains, one molecule of flavin mononucleotide(FMN),…
Q: enzyme that converts succinate to fumarate, uses ____ as its coenzyme. FADH2 FAD…
A: Succinate to fumarate reaction- It occurs in the citric acid cycle
Q: An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate)…
A: We need to draw the Lineweaver Burk (LB) plot to find out the answers. LB plot has 1/[S] as X-axis…
Q: OH HO- CH₂OH OH -CH-CH₂ HO-CH- . CH I NH " CH . C=0 (СН2)12 (CH2 10CH3
A: The given molecule has a polar head group in the form of a carbohydrate or sugar moiety and…
Q: 4. Fill in the table below Pathway Number of ATP produced from one glucose molecule Glycolysis Krebs…
A: The glycolytic pathway synthesizes energy through the metabolism of glucose. The acetyl CoA…
Q: Which of these processes is most exergonic? (Think VERY carefully!) Dephosphorylation of…
A: Exergonic reactions are the ones, which release energy to the surroundings. The energy is released…
Q: 10.12 2. What is the clinical significance of ALT?
A: Alanine aminotransferase, usually referred to as ALT, is an enzyme that is concentrated primarily in…
Q: Suggest the possible class of enzyme (or name of enzyme) for each of the enzyme-catalyzed reactions…
A: Introduction: Enzymes are biological catalysts that fasten the rate of the biochemical reaction.…
Q: Using the genetic code, indicate which polypeptides would be synthesized if poly-UGG were used in a…
A: Polypeptides are the linera chain of Amino acids Synthesised according to the codons on mRNA.…
Q: 6. Explain br
A: Enzymes have an important role in human beings. Enzymes have a tendnecy to speed up the reactions.…
Q: Polypeptide synthesis proceeds in either direction once initiated. proceeds from the…
A: Peptides are short chains of amino acids that are linked together by means of a peptide bond.…
Q: 2) An individual plasma [Na+] is measured and found to be 130 mEq/L (normal = 145 mEq/L). What is…
A: Osmolarity and osmolality are both used to represent concentration of solute in the solves the…
Q: If Dr. Aikenhed wanted to see if there was mutation within the protein-coding sequence of the gene…
A: The technique which can be used to compare protein-coding sequence i.e the nucleotide sequence of…
Q: Are tRNAfmet and tRNAmet made from the same aminoacyl tRNA synthetase?
A: Amino acyl tRNA synthase pairs tRNA with amino acid that their anti codon codes for. Amino acyl tRNA…
Q: Write an analysis of the results, interpreting what you see in the smaller gell focusing on 6A, 6B,…
A: DNA fingerprinting is a technique which enables us to identify an individual via his unique Genome…
Q: escribe the catalytic reaction mechanism of the enzyme acetylcholinesterase. Discuss the functional…
A: Acetylcholinesterase(AChE) is an enzyme found in the synapses between nerve cells and muscle cells.…
Q: Indicate what will happen (increase, decrease or no effect) to the activity of enzyme or rate of the…
A: Glycogen synthase is an enzyme essential for the synthesis of glycogen from glucose residues.…
Q: Molecule Amino Acid (Glycine R=H) Glycerol a-glucose Molecular Formula Variable C2H5NO2 Structural…
A: Biomolecules are carbon-based organic compounds produced by living organisms. They are primarily…
Q: Peptide (amide) bonds are foundational to biochemistry. Use the instructions for drawing peptides to…
A: chain of amino acids are linked by peptide bonds and physiological pH, the C-termini and N-termini…
Q: Generation of induced pluripotent stem (iPS)cells was first accomplished using retroviral vectors…
A: Induced pluripotent stem cells that are also referred asiPSCs are generated only from…
Q: Which of the following is not released during alcoholic fermentation? NAD+ CH3CH2OH…
A: Alcoholic fermentation is a process where yeast converts sugar into alcohol. It involves various…
Q: Why is it important to lower the temperature of the chicken liver at the initial stages of glycogen…
A: During the dissection of an animal's liver, it is important to provide the septic conditions to…
Q: What are the x and y values that you have input in the excel? I thought the x would be from the…
A: Proteins are composed of amino acids, coomassie brilliant blue dye binds to basic amino acids of the…
Q: What positive impact can negatively charged amino acid in a protein have in the body? Include an…
A: Answers for the following question are :
Q: Acetyl-CoA, AG" = -7.7 kcal/mol CoA Oxaloacetate Citrate AG" = ? AG"=-1.5 kcal/mol Isocitrate Krebs…
A: The tricarboxylic acid (TCA) cycle, or the Krebs or citric acid cycle, is the primary source of…
Q: A hamster receives a dose of 3 mg. of a compound and then 2/3 as much as the previous dose at the…
A: Regardless of the reason for administration, drug dosing must be safe and effective. Allometric…
Q: Which of the following indicates buffering in the titration given in the image below? 12 11 10 Point…
A: A buffer is a aqueous solution which is used to resist the pH change upon addition of acid or base.…
Q: The pentose phosphate pathway: Produces heat through simultaneous activation of both catabolic…
A: Pentose phosphate pathway is a metabolic pathway. It takes place in plastids in plant cell while in…
Q: Which of the following is TRUE, if the protein pH is electrically neutral? Refer to the graph…
A: Protein denaturation is the process where protein loses its secondary conformation due to extreme…
Q: I really dont understand this one I hope you can help me with this. Thank you so much. It has a 10…
A: The excess glucose molecules present in the blood are stored in the form of glycogen in the liver…
Q: 1. How many NADH _____& ATP _____are produced from the beta oxidation of Lauric Acid? 2. How many…
A: Most fatty acids are degraded by sequential removal of two-carbon fragments from the carboxyl end…
Q: What are polymorphs in pharmacy? Discuss the effects of polymorphism on the physico-chemical…
A: Morphology means physical form, the shape or structure of something. When more than one form exist…
Q: Which of the following is TRUE about the concept of chromoproteins? Myoglobin is mostly concentrated…
A: Introduction: Chromoproteins are proteins with colored prosthetic groups. For example, cytochrome…
Q: Enzyme elastase cleaves polypeptides after ______________ aa. small Positively charged…
A: Enzymes such as elastases have a tendency to leave after aliphatic amino acids. These amino acids…
Q: Give at least two common loading control used in Western Blotting?
A: Western blotting is a laboratory technique that is used to detect a specific protein in a a…
Q: Draw a schematic diagram on glycogenolysis by showing the structural formula of its substrate. Show…
A: Glycogen is mainly the "storage" form of carbohydrates present in mammals. Majorly in humans, the…
Q: Which of the following contains isoprene units?
A: The terpenes are monoterpenes are compounds that contain two isoprene molecules.
Q: The major intrinsic protein responsible for the passage of water through cell membranes is O…
A: Introduction: A membrane protein is a protein molecule that is attached or associated with the…
Q: Based on the quantitation data, please indicate how you would dilute the following samples. Fill in…
A: DNA shows maximum absorbance at the wavelength of 260 nm. Hence the concentration of DNA in solution…
Q: Question: Glutamic acid is the parent compound of monosodium glutamate (known as MSG), which is used…
A: Glutamic acid is a polar acidic amino acid. In addition to the amino and carboxyl groups attached to…
Q: C4 plants have higher photosynthetic rates than C3 plants because they have an additional CO2-fixing…
A: Photosynthesis means the reduction of carbon dioxide to carbohydrate or glucose and the reduction of…
Q: xplain briefly but concisely 3. Mammalian liver can carry out gluconeogenesis using oxaloacetate as…
A: Gluconeogenesisis the process that requires energy. It is not a simply the reversal of process of…
Q: How might defective mitochondria lead to cancer?
A: Most eukaryotic species have mitochondrion, which is a double-membrane-bound organelle. Mitochondria…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- From the figure of O2 binding to myoglobin and hemoglobin (ignore the linemarked as T) as described in lecture (shown below) answer the following questions. a) Estimate the P50 for myoglobin from the plot. Show how this estimation isdetermined from the binding curve above. ( The first ghraph) b)Using YO2 = PO2/P50 + PO2 , calculate the fraction of O2 bound for myoglobin at 1 torr. (2nd graph) c)Using the binding curve on the previous page, show how you can estimate whatfraction of hemoglobin is bound near tissues at a pO2 of 30 torr and provide this value. If the pH were lowered, will the amount of O2 bound to hemoglobin at 30 torr increaseor decrease? Explain why this is so based on how this changes hemoglobin structure. If 2,3-BPG were added to the solution, will the amount of O2 bound to hemoglobin at30 torr increase or decrease? Explain why this is so based on how this changes hemoglobinstructure.A patient carrying a mutant form of hemoglobin (KD = 48 torr) is planning to take part in a hiking trip that involves strenuous physical activity at ~10,000 feet above sea level. The mutant hemoglobin has reduced oxygen binding cooperativity (n = 2.2) and displays no major structural abnormalities. a. Calculate the percent saturation of hemoglobin in the lungs (pO2 = 70 torr at this elevation) for this patient and for an individual carrying a normal version of hemoglobin b. Calculate the percent saturation of hemoglobin in active skeletal muscle tissue (pO2 = 15 torr) for this patient and for an individual carrying a normal version of hemoglobin c. Will this patient transport oxygen from the lungs to active muscle tissues more or less efficientlythan an individual with a normal version of hemoglobin on this trip? Briefly explain your answerBelow is an oxygen-binding curve for two hemoglobin variants. 1.0 0.8 0.6 B 0.4 0.2 20 40 60 80 100 O2 pressure (torr) Determine the p50 for variant A to the nearest 5 torr (i.e., if the p50 was 12, you would write 10). Determine the fraction of the hemoglobin B bound with O2 in the lungs (pO2 = 100 torr) that delivers oxygen to the tissues (pO2 = 20 torr)? Answer to the nearest 0.05 value (for example, 0.10 or 0.15). Which hemoglobin variant (write A or B or same) delivers more oxygen to tissues? Fraction bound with O2
- The O2-binding curves for normal haemoglobin (Hb A) and a mutant haemoglobin (Hb Kempsey) are shown in the diagram. Which of the following statements, based on this graph, are true? 1.0 0.8 Hb/Kempsey 0.6 Hb A 0.4 0.2 0.0 20 40 60 80 100 p02 (torr) 1. Hb Kempsey has a higher affinity for O, than HbA 2. Hb Kempsey is unable to transition between the R and T states 3. Hb Kempsey demonstrates cooperative binding of oxygen. 4. Hb Kempsey's curve could be explained by a loss of nearly all the cooperative interactions between the subunits. 5. A possible explanation for the curve observed with Hb Kempsey is mutation at His HC3. 2,4,5. O 1.2,5. O 1,2,3,4,5. O 2,3,4. O 1.2.4. Y (Fraction Saturation)Below is a fractional saturation curve for O₂ binding to adult hemoglobin. Assume that curve Y represents a condition at pH 7.4 and with a normal physiological level of 2,3-BPG. Curve X represents a condition that Fractional saturation (0) 0.2 0.0 0 Tissue PO, (kPa) 12 LungsWhich option best describes the following figure? Y (fraction saturation) 1.0 0.8 0.6 0.4 0.2 0.0 0 20 Oxygen Binding plot 40 60 p02 (torr) 80 100 A) Curve x = adult haemoglobin, curve y = fetal haemoglobin B) Curve x = myoglobin, curve y = haemoglobin C) Curve x = fetal haemoglobin, curve y = adult haemoglobin D) Curve x = fetal haemoglobin, curve y = myoglobin E) Curve x = adult haemoglobin, curve y - myoglobin
- Help me pleaseIn a study of O2 uptake by muscle at high altitude, a physiologist prepares an atmosphere consisting of 79 mole % N2, 17 mole % 16O2, and 4.0 mole % 18O2. (The isotope 18O will be measured to determine O2 uptake.) The total pressure is 0.75 atm to simulate high altitude. Calculate the mole fraction and partial pressure of 18O2 in the mixture.These are performance curves for two oxygen binding proteins. While we didn't go over the curves themselves in lecture, what are the axis labels? Myoglobin 0.8 0.6 V 0.4 0.2 I 0.0 25 50 1.0 Hemoglobin P50 = 26 torr 75 I 100 The y-axis is the fractional saturation of the protein by oxygen The x-axis is related to the concentration of oxygen to which the protein is exposed. The y-axis is the concentration of oxygen in the air. The x-axis is the fractional saturation of the protein by oxygen
- Below is the O₂ binding curve for adult Hb in whole blood (containing BPG) shown in red (labelled Hb). Fraction of Hb bound with Oxygen 1.0 0.8 0.6 0.4 0.2 0.0 0 20 40 pO2 (torr) 60 80 ABC c. Which curve lacks cooperativity? [Select] Hb 100 a. Which curve is the best representation for stripped Hb (pure hemoglobin with heme, but no other small allosteric molecules associated)? [Select] b. Which curve has the lowest affinity (as measured by the p50)? [Select]Hemoglobin and myoglobin both use heme as their prosthetic group and they both bind O2. However, their O2-binding curves indicate these proteins have different activities. What lind of information can be learned about the differences between the curves?Suppose Gina climbs a high mountain where the oxygen partial pressure in the air decreases to 70 torr. Assume that the pH of her tissues and lungs is 7.4 and the oxygen concentration in her tissues is 20 torr. The P50 of hemoglobin is 26 torr. The degree of cooperativity of hemoglobin, n, is 2.8. Estimate the percentage of the oxygen-carrying capacity that she utilizes. Calculate your answer to one decimal place. сараcity: %