The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of Phenylthiourea. All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van Phenylthiourea. Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. 1,01 0,49 0,32 0,25 0,19 [catechol] (mM) Rate (AAU/min) Uninhibited 65,02 47,11 38,44 32,84 26,92 Rate (AAU/min) Phenylthiourea (1.5mM) 46,62 37,45 31,10 27,40 23,36 Using graphical methods, determine what type of inhibitor Phenylthiourea is, as well as the inhibited reaction's K'm and V'max. Following this, determine Phenylthiourea's Ki (also using graphical methods). Please note, the Lineweaver-Burk plot for the uninhibited reaction has been provided. Print it out or recreate it and use it to determine the above-mentioned. Met behulp van grafiese metodes, bepaal watter tipe inhibeerder Phenylthiourea is, asook die geïnhibeerde reaksie se K'm en V'max. Bepaal hierna Phenylthiourea se Ki (ook met behulp van grafiese metodes). Neem asseblief kennis dat die Lineweaver-Burk plot vir die ongeïnhibeerde reaksie verskaf is. Druk hom uit of teken hom oor en gebruik hom om die bogenoemde te bepaal. created with craft
The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of Phenylthiourea. All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van Phenylthiourea. Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. 1,01 0,49 0,32 0,25 0,19 [catechol] (mM) Rate (AAU/min) Uninhibited 65,02 47,11 38,44 32,84 26,92 Rate (AAU/min) Phenylthiourea (1.5mM) 46,62 37,45 31,10 27,40 23,36 Using graphical methods, determine what type of inhibitor Phenylthiourea is, as well as the inhibited reaction's K'm and V'max. Following this, determine Phenylthiourea's Ki (also using graphical methods). Please note, the Lineweaver-Burk plot for the uninhibited reaction has been provided. Print it out or recreate it and use it to determine the above-mentioned. Met behulp van grafiese metodes, bepaal watter tipe inhibeerder Phenylthiourea is, asook die geïnhibeerde reaksie se K'm en V'max. Bepaal hierna Phenylthiourea se Ki (ook met behulp van grafiese metodes). Neem asseblief kennis dat die Lineweaver-Burk plot vir die ongeïnhibeerde reaksie verskaf is. Druk hom uit of teken hom oor en gebruik hom om die bogenoemde te bepaal. created with craft
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl
oxidase. Additional data is also provided for when the reaction is run in the presence of Phenylthiourea. All
data was acquired by monitoring the change in absorbance at 540 nm.
Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o-
difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die
teenwoordigheid van Phenylthiourea. Alle data is verkry deur die verandering in absorpsie by 540 nm te
monitor.
1,01
0,49
0,32
0,25
0,19
[catechol] (mM)
Rate (AAU/min)
Uninhibited
65,02
47,11
38,44
32,84
26,92
Rate (AAU/min)
Phenylthiourea (1.5mM)
46,62
37,45
31,10
27,40
23,36
Using graphical methods, determine what type of inhibitor Phenylthiourea is, as well as the inhibited
reaction's K'm and V'max. Following this, determine Phenylthiourea's Ki (also using graphical methods).
Please note, the Lineweaver-Burk plot for the uninhibited reaction has been provided. Print it out or
recreate it and use it to determine the above-mentioned.
Met behulp van grafiese metodes, bepaal watter tipe inhibeerder Phenylthiourea is, asook die
geïnhibeerde reaksie se K'm en V'max. Bepaal hierna Phenylthiourea se Ki (ook met behulp van grafiese
metodes).
Neem asseblief kennis dat die Lineweaver-Burk plot vir die ongeïnhibeerde reaksie verskaf is. Druk
hom uit of teken hom oor en gebruik hom om die bogenoemde te bepaal.
created with craft](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0f8f3967-2238-4d0b-a9b9-a8ca0723a7bd%2F6d027ad9-2d0d-41de-bfee-b8689b4aa2d2%2Fcpy9sda_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl
oxidase. Additional data is also provided for when the reaction is run in the presence of Phenylthiourea. All
data was acquired by monitoring the change in absorbance at 540 nm.
Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o-
difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die
teenwoordigheid van Phenylthiourea. Alle data is verkry deur die verandering in absorpsie by 540 nm te
monitor.
1,01
0,49
0,32
0,25
0,19
[catechol] (mM)
Rate (AAU/min)
Uninhibited
65,02
47,11
38,44
32,84
26,92
Rate (AAU/min)
Phenylthiourea (1.5mM)
46,62
37,45
31,10
27,40
23,36
Using graphical methods, determine what type of inhibitor Phenylthiourea is, as well as the inhibited
reaction's K'm and V'max. Following this, determine Phenylthiourea's Ki (also using graphical methods).
Please note, the Lineweaver-Burk plot for the uninhibited reaction has been provided. Print it out or
recreate it and use it to determine the above-mentioned.
Met behulp van grafiese metodes, bepaal watter tipe inhibeerder Phenylthiourea is, asook die
geïnhibeerde reaksie se K'm en V'max. Bepaal hierna Phenylthiourea se Ki (ook met behulp van grafiese
metodes).
Neem asseblief kennis dat die Lineweaver-Burk plot vir die ongeïnhibeerde reaksie verskaf is. Druk
hom uit of teken hom oor en gebruik hom om die bogenoemde te bepaal.
created with craft
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