The following carbohydrate tests have been performed on each of the following sugar standard solutions. Indicate which of the sugar standards will give a positive (+) or negative (-) reaction for each of the following tests. Answer by entering either a plus (+) or negative (-) sign in each cell of the table below. Fehling Benedict Barfoed Bial Seliwanoff lodine Xylose Glucose Fructose Sucrose
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- Give one test to show the difference between reducing and non-reducing sugars. (Give the indication of positive test, such as color, precipitate formation, etc.) Is sucrose a reducing or non-reducing sugar? Explain. Is lactose a reducing non reducing sugar? Explain.80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Fehling’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)
- Draw Haworth projection formulas for the b-anomer of monosaccharides with each of the following Fischer projection formulasdo the calculution for the prepartion of 100mg/ L diclofenac solution, containing 100mg/L of caffeine as an internal standard, in acetonitrile from a stock solution.Given Tagatose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s test
- An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by ion-exchangechromatography, using a cation-exchange resin at pH 3.5, with theeluting buffer at the same pH. Which of these amino acids will beeluted from the column first? Will any other treatment be neededto elute one of these amino acids from the column?Upon examining the test results for the unknown carbohydrate from all the tests depicted in the images, what are some of the identities of the unknown carbohydrate? Is it one of the sugars from the list in the Reducing or Nonreducing sugars test? if not what carbohydrate is it?Tirofiban (C22H36N₂O5S MWt = 440.6) is present as tirofiban HCI monohydrate MWt=495.1) at 0.281 mg/mL in a concentrated solution. (C22H36N2O5S.HCI.H₂O A solution for infusion is prepared by extracting 50 mL from a 250 mL bag of 5% glucose s and adding 50 mL of concentrated solution. Sonya who weighs weighs 60 kg requires a tirofiban dose of 0.4 mcg/kg/min for 30 minute- What volume would remain after 30 minutes? Answer: 94.53 mL X The correct answer is: 236 mL
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)Please answer both of the following 1) The Haworth projection of D-altrose is shownWhat type of cyclic sugar is formed? 2)Which of the following statement is true about the quaternary structure of aprotein? only one answer is correctAn amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?