The concentration of the pesticide Imazalil should be determined in an apple sample. Outline how you will proceed to analyze Imazalil in the apple sample: Extraction and purification, concentration and analysis (chromatographic technique, column, detector, mobile phase, etc.). Imazalil is a thermally stable substance with a melting point of 50 ° C. The compound is sparingly water soluble (1.4 g/ L) with LogP = 3.8. The compound is further weakly basic with a pka value equal to 6.5 The structure of Imazalil (C14H14CI2N2O). The compound is weakly basic, pKa (NH +) = 6.5.
Q: Describe the sample properties that would be suitable for; i) Gas chromatography (GC) analysis ii)…
A: GS and HPLC are the techniques to separate compounds from mixtures .These are nalytical methods of…
Q: Help please : When comparing HPLC with Gas Chromatography , Gas Chromatography analysis may require…
A: Chemical derivatization is a step in gas chromatography in which a compound is chemically…
Q: Which statements are true for molecular exclusion chromatography?I Molecular exclusion…
A: ✓As the name ,Size-exclusion chromatography, also known as molecular sieve chromatography, is a…
Q: Which of the following could be a consequence of an open and uncovered chromatographic chamber?…
A: TLC is an analytical technique which can be used to monitor the organic reaction transformations.…
Q: 30) The separation efficiency of capillary columns over packed columns 31) The most common mobile…
A: Ans. (30) The separation efficiency of capillary columns over packed columns in GC is attributable…
Q: Jörgen et al. Extracted acrylamide from potato chips by soxhlet extraction. They used methanol as a…
A: From the unit (hours) given it can be concluded that time has been plotted in X-axis.
Q: In ion-exclusion chromatography, ions are separated from nonelectrolytes (uncharged molecules) by an…
A:
Q: Samples X and Y were analysed for Na by flame emission photometry. A series of standard solutions…
A: A question based on analytical process that is to be accomplished.
Q: Pesticide residues were found in a lot of oranges; the compound identified was the epoxide of…
A: Given data:
Q: What are the properties that determine retention time in the Chromatography process? Please shortly…
A: Retention time is interval between the sample injection and detection of substance. The…
Q: 6. If more polar impurities are desired to be eluted first and then analyte, then at which stage do…
A: 6. If more polar impurities are desired to be eluted first and then analyte, then at step (c) i.e.…
Q: To determine the soil distribution coefficient (Kd), 10 g of dry soil is spiked with 100 mg of…
A: The distribution coefficient Kd can be calculated using the expression: Kd=Atrazine present in…
Q: What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for Analysis of…
A: a). HILIC. Because there will be occuring hydrophilic intraction chromatography also it can partly…
Q: Which of the following statements about the setup of thin layer chromatography is FALSE? The…
A:
Q: how would you sample, preserve and prepare these samples for GC analysis and why GC is the best…
A: By injecting a gaseous or liquid sample into a mobile phase, which is frequently referred to as the…
Q: hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one…
A: Concept- In thin layer chromatography, the distance from the baseline of the given compound divided…
Q: Classify the stationary phases based on whether they are commonly used in reversed-phase liquid…
A:
Q: State why calibrators and control for spectophotometry should be purchased in addition to the…
A: Interpretation - To state why calibrators and control for the spectrophotometry should be purchased…
Q: what are key reactions involved in seperation of liquid mixtures by simple and fractional…
A: Introduction: Chromatography and distillation is a technique used to separate the mixture of…
Q: A flavor chemist attempted to isolate and identify the major flavor component of a recently…
A: IR : 3100 cm-1 sp2 C-H stretch, 2950 cm-1 sp3 C-H stretch, 1735 cm-1 carbonyl stretch of ester, 800…
Q: Gestation time arrangement of Hegran, Hexanol, and Benzene compounds in the reverse phase of column…
A: A question based on analytical process that is to be accomplished.
Q: What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for Analysis of…
A: a). HILIC. Because there will be occurring hydrophilic interaction chromatography also it can…
Q: B. There was big news all over the internet that the Ponkan oranges that were imported from one of…
A: Gas chromatography: Gas chromatography is an analytical technique used to seperate the…
Q: 1. Define retention factor. Explain why it is or isn't an intrinsic property of a substance. 2. A…
A: TLC (Thin layer chromatography) is a technique used to separate the components in a mixture using a…
Q: 1. State the reason why we need to compare the observed retention time values to the standard values…
A: As per the rules, only the first question can be answered. Retention time is the time spent by the…
Q: Choose the separation techniques that rely on differences in boiling points. A. Simple distillation…
A: To identify: The techniques which use the difference in boiling point for separation.
Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…
A:
Q: Which of the following is TRUE for a reversed phase chromatography experiment? i. Less polar…
A:
Q: An open tubular chromatography column is 28.7 m long with an inner diameter of 0.503 mm . The inside…
A: Note -Since you have posted a question with multiple sub-parts, we will solve first three sub-parts…
Q: 0.56 g 1,4‐dimethoxybenzene, 0.9 mL 2‐chloro‐2‐methylpropane, 5 mL nitrobenzene and 0.52 g graphite…
A: The given problem is related to green chemistry. Given: Reactants- Mass of 1,4‐dimethoxybenzene-…
Q: A brewed tea brand sample was subjected to solvent extraction to extract caffeine. The cup of tea…
A:
Q: A sample of Melbourne drinking water was injected into an ion chromatograph. The anions were…
A: Given: Void time = 1.25 min ts for chlorides = 3.12 min ts for sulfate = 6.62 min
Q: A student used thin layer chromatography to confirm whether he could successfully separate two…
A: TLC means “Thin Layer Chromatography”. This experiment is done on Wattman paper. If the molecule has…
Q: A flavor chemist attempted to isolate and identify the major flavor component of a recently…
A: Given information: The isolated compound was dissolved in ethyl acetate and first extracted with 0.1…
Q: 1. What are the features of samples/compounds that allow them to be suitable for Gas Chromatography…
A:
Q: Briefly explain four importance of conducting sample preparation prior to instrumental analysis.
A: Sample preparation is a very crucial step in experimental analysis . This is one of the most…
Q: Lalisa and Labmi were tasked to design a new and efficient method of producing black inks. To…
A: Retardation or retention factor (Rf) value is the ratio of distance traveled by the analyte to that…
Q: 1. The properties that must be possessed by the adsorption reagent in air sampling are… a. Reagents…
A: Air sampling is the process by which the type and quantity of airborne contaminants present in an…
Q: Which of the following cannot be used as adsorbent in column chromatography? a. Silica gel…
A:
Q: If there is a mixture of more volatile and less volatile substances and are to be separated through…
A:
Q: Which among the following applies to reversed phase chromatography? i. Compounds of low polarity…
A: The correct answer about reversed phase chromatography is given below
Q: Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum,…
A:
Q: 5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on…
A: To answer question 5.
Q: What chromatographic technique is used to identify and quantify volatile substances? Paper…
A: Chromatography is a technique to separate components of a given mixture. There are many types of…
Q: The following statements are true for any chromatographic analysis except: The analyte component…
A:
Q: Question 6 Which is NOT a factor that affects retention time? I. the pressure used II. The nature of…
A: The retention time will vary depending on: 1.The pressure used (because that affects the flow rate…
Q: Extraction of the aqueous salicylic acid solutions with 10% ethyl acetate/hexane (density ~ 0.7…
A: As you have posted two independent questions in a single request, we will solve the first question…
Q: Which type of chromatography is paper chromatography? a. Liquid-liquid chromatography b.…
A: Chromatography is a technique used to seperate components from a mixture. Here we have to tell what…
Q: Why ebuilliometer is having a great effect on measuring ethanol Yield than gas chromatography
A: Ebulliometry is based on the principle that the boiling point of wine is depressed in comparison to…
Step by step
Solved in 3 steps
- 16) Which of the following(s) is/are correct? I- Selectivity factor is the amount of time a solute spends in the stationary phase relative to the time it spends in mobile phase II- The retention time for an analyte is the time interval between its injection onto a column and its appearance at the detector at the other end of the column. III- Distribution constant is the ratio of the total solute concentration in the stationary phase to that in the mobile phase. A) I, II and III B) Only II C) I and III D) II and III E) Only III for GC2Accurately weigh out about 6g copper(ii) sulfate crystals into a weighing boat. Use the copper(ii) sulfate crystals to make up 250cm3 of standardized copper (ii) sulfate solution Pipette 25cm3 of this solution into a conical flask Add 1.5g potassium iodide and swirl thoroughly Titrate this solution with standard 0.1 moldm-3 Na2S2O3 in a burette. When the iodine color fades, add 1 cm3 starch indicator. Use your titration data below to calculate the percentage by mass of copper in the copper(ii) sulfate crystals.Below is a chromatogram of a mixture of phenolic pollutants obtained from the analysis of a river water sample using HPLC reversed-phase chromatography. Based on this chromatogram, answer the following questions. (i) (ii) (iii) (iv) 0.01 TO 0.8 1.2 0". BEO - 2.0 1757 $122. 2.8 3.2 What is meant by 'retention time'? Calculate the capacity factor, k, for compounds 1 and 2. Calculate the selectivity factor, a, for compounds 1 and 2. Suggest two parameters (or factors) that may affect the selectivity factor, a, for the two compounds?
- A simple paper chromatography experiment involved the analysis of the food dyes in lollipop samples. A chocolate-flavored lollipop was dissolved using various solvents and was spotted in the chromatogram (E). Four food dye standards (blue, green, red, and orange) were tested alongside the chocolate-flavored lollipop. The mobile phase used for the analysis was 0.1% NaCl. After the analysis, the resulting chromatogram revealed the separation of three colors, which were attributed to the food dyes indigo carmine (blue), tartrazine (yellow), and erythrosine (red). 10 Ruler is in cm unit в с B C D A E A E BEFORE AFTER Calculate the rf for each dye. Express answers in 3SF. erythrosine: indigocarmine: tartrazine: Which dye is the most polar? Choose.. Which dye is the least polar? Choose...6.0 mL of cyclohexanol, 8 mL of 85% phosphoric acid, and 10 drops of sulfuric acid are added to a 50-mL round-bottom flask with boiling chips, and the mixture is distilled into an ice-cooled receiver. The distillate is in the form of a colorless bi-phasic mixture. The cyclohexene upper layer is removed from the water layer, and dried over anhydrous calcium chloride. Isolated is cyclohexene as a clear and colorless foul-smelling liquid. Weighing data is given below. Gross mass: 51.24 g Tare mass: 48.04 g Product mass: 3.2g Question: Calculate the theoretical mass of the cyclohexene product, bearing in mind that the cyclohexanol starting material was measured by volume, not by mass (so you must use the density of cyclohexanol to convert milliliters to grams, before calculating the number of moles). Calculate the percent yield of cyclohexene obtained.Which statement about the determination of the percentage purity of benzoic acid is correct? * A- Only sodium hydroxide solution can be used as the titrant in the volumetric analysis of benzoic acid. B- Analysing a benzoic acid sample that still contains some hydrochloric acid may yield an analyte percentage that is less than expected. C- Analysing a wet benzoic acid sample may yield an analyte percentage that is more than expected. D- All these statements are correct. E- None of these statements are correct.
- From a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL. From this, a 5-mL aliquot was taken and diluted to 20mL.The final 20mL was found to have a concentration of 0.004M analyte X.What is the concentration of analyte X in the 10-mL sample?A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 301 and the area of peak B is determined to be 349 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.41 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a 10 Time (min) 15 peak area of 499 for peak A and 417 for peak B. What is the concentration of methyl benzoate in the plant stream? methyl benzoate concentration: mg/mL Detector ResponseTo determine the soil distribution coefficient (Kd), 10 g of dry soil is spiked with 100 mg of ring-labeled 14C-atrazine, 10 mL of deionized water is added, and the system is shaken for 24 hours. The aqueous and solid phases are separated, and the solid phase is extracted with 50 mL of methylene chloride while the aqueous phase is extracted with three successive 5 mL portions of methylene chloride. The atrazine concentration in the 50 mL of soil extract is 10 mg/L, and the concentration in the combined 15 mL from the aqueous extractions is 0.4 mg/L. Based on this information, calculate Kd.
- A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 3.40×102 and the area of peak B is determined to be 398 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.49 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 437 for peak A and 415 for peak B. What is the concentration of methyl benzoate in the plant stream?A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.31 mg/mL of methyl benzoate (peak A) and 1.44 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 352 and the area of peak B is determined to be 395 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.21 mg/ml is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 473 for peak A and 421 for peak B. What is the concentration of methyl benzoate in the plant stream? methyl benzoate concentration: 2.7 incorrect Detector Response 10 Time (min) 15 mg/mL9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B (pyridoxine hydrochloride) are determined by capillary zone electrophoresis (CZE) using a pH 9 sodium tetraborate-sodium dihydrogen phosphate buffer or by micellar electrokinetic chromatography (MEKC) using the same buffer with the addition of sodium dodecyl sulphate. Detection is by UV absorption at 200 nm. CH₂OH pka 5.5 pka 9.7 ΝΗ -OH HOH₂C. N NH₂ pKa 3.6 NH₂ HO -OH Vitamin B3 (weakly basic) Vitamin B6 (weakly acidic) Vitamin B₁ (weakly basic) HO- CH₂OH Vitamin B₂ (weakly acidic) a The elution order when using MEKC is vitamin B3 (5.59 min), vitamin B6 (6.60 min), vitamin B₂ (8.82 min) and vitamin B₁ (11.22 min). Comment on the solubility of the B vitamins in the sodium dodecyl sulphate micelles? The sodium dodecyl sulphate micelles elute at 17.8 min. OH pka 9.0