Task #3: Design primers1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg121 cctctgccgg gaggagcgag gggaccqcag gcgggggcgc atgacctcag gagccgcgcc181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt241 atttctacac ctccgtgtcc cggcccqgcc gcggggagcc ccgcttcatc tcagtgggct301 acgtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc481 agagcgaggc cgttgcqtga ccccqgcccg gggcgcaggt cacgactccc catcccccac541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccgcgggaa) First you'll need to design primers to PCR-amplify amino acids 45-56.i)Record the sequences of the forward and reverse primers, in the 5' to 3' direction. Eachprimer must be 8 nucleotides long (note that normally primers are much longer than this).Show intermediate steps in your primer design process and/or explain how you determinedthe primer sequences.ii) Using the method described in the pre-tutorial material, calculate the Tm for each primer (in°C).

A primer is a short single-stranded nucleotide chain used for the initiation of DNA synthesis. In…

Answered: Task #3: Design primers 1 agagtctcct…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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AGTGCAGCTC CAGCCGACCC CCGGGACGTA GACAAGGGCA GGCGCGCGGT 50 GAAGACTGCG GCCGGCCGCG TAGCCGCGCT GTGGTCCCGC TGGCCTTCTC 100 TTGGGCCGCG CACCCTCCGG ACCTGCGTCG GGATCCTCCC GGAGTCTCCC 150 GCTCTCTCCT CCCGGCTTGC GAACATGCGG CCCCTTAAGC CCGGCGCCCC 200 TTTGCCCGCA CTCTTCCTGC TGGCGCTGGC TTTGTCCCCG CACGGAGCCC 250 ACGGGAGGCC CCGGGGGCGC AGGGGAGCGC GCGTCACGGA TAAGGAGCCC 300 AAGCCGTTGC TTTTCCTCCC CGCGGCCGGG GCCGGCCGGA CTCCCAGCGG 350 CTCCCGGAGC GCAGAAATAT TCCCAAGAGA CTCTAACTTA AAAGACAAAT 400 TCATAAAGCA TTTCACAGGG CCGGTCACAT TTTCACCAGA ATGCAGCAAA 450 CATTTCCACC GACTCTATTA CAATACCAGG GAGTGCTCAA CGCCAGCTTA 500 TTACAAAAGA TGTGCTAGAT TGTTAACAAG ATTAGCAGTG AGTCCACTGT 550 GCTCCCAGAC CTAGCAAAAC TACCCTACAT TTCCTAAGAA TGTACATCTA 600 ATTTGAAGAA AAAGTGCCTC AAATCATGCA AAATGTAAAA AAAGATGAAA 650 TTTATATTTT TATGGATATT AAGATGAGTA AAATAAGAGA CTTCCCAGAA 700 ATAACTGGTT AGCTGTTTCC TGTCATAGAA TGGAGTCTTT CTTGCTTTAT 750 CTTTTTGTGT ATACAGTAAT TTATAATTTT GTAAAACAGA GTTTGAATCG 800 CATATTGAAA ATTAGATATT AAAAATTGTG…
cggcaggagc cccccgacct cccaggcgga ccgccctccc tccccgcgcg cgggttccgg gcccggcgag agggcgcgag cacagccgag gccatggagg tgacggcgga ccagccgcgctgggtgagcc accaccaccc cgccgtgctc aacgggcagc acccggacac gggcctcagcc actcctacat ggacgcggcg cagtacccgc tgccggagga ggtggatgtg ctttttaaca tcgacggtca aggcaaccac gtcccgccct actacggaaa ctcggtcagg Provide the primers for the nucleotide sequence above (GATA3)gene
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Second letter UUU UUC UUA UUG. UCU UCC UCA UCG UAU Tyr UAC. Ser UAA Skp UGIA Stop UGU UGC. Phe Cys Leu UAG Sop UGG Trp CGU CGC Arg CGA CCU CAU CUU CUC CUA CUG His CAC CAA CAG Gin CCC Pro CCA Leu CCG CGG ACU AAUA AGU ]Ser AUU Asn AAC AGC AGA LArg AGG. AUC Be ACC ACA Thr AAA AUA AUG Met ACG MGys GUU GUC Val GUA GCU GCC GCA GCG Ala GAA Glu GAG GAU GAC Asp GGU GGC Gly GGA GGG GUG You come across four polynucleotide strands. The first is an original RNA strand that codes for a protein; the others are similar to the original RNA strand, except that each displays a point mutation. The point mutations are shown in red. 5' - AUG-UGC-AUU-CCU-GCA-UAA - 3' 5' - AUG-UGA-AUU-CCU-GCA-UAA - 3' 5' - AUG-UGC-AUU-CCC-GCA-UAA - 3' 5' - AUG-UGC-GUU-CCU-GCA-UAA - 3' Original RNA: Mutant RNA 1: Mutant RNA 2: Mutant RNA 3: 1. Based on how they will affect the protein product, identify the type of point mutation (missense, nonsense, silent, or frameshift) present in the Mutants 1, 2, and 3. 2. State which of…
C A UUU U UUC Phe UCU UCC UCA UAU UAC UAA UAG UGU Cys U UGC Stop UGA Stop Stop UGG Trp G Tyr Ser UUA UUG Leu UCG CUU C CUC CUA CCU Leu ccc CCA CCG CAU CAC CAA His CGU CGC CGA CGG Pro Arg 1st Gin CUG CAG 3rd letter AUU A AUC AUA AUG ACU AAU AAC Ser u letter C Asn AGU AGC AGA AGG lle ACC Thr ACA AAA AAG Lys Arg Met ACG GUU G GUC GUA GUG GCU GCC GCA GCG GAU GAC GAA GAG Asp GGU GGC GGA GGG Val Ala Gly Glu Not all of the following ingredients are necessary for an in vitro translation reaction. Which one would you leave out? A ssDNA template O Cell sap containing ribosomes poly(U) O 14C-Phe
ule_2023_Spring_Stu x M Inbox (1) - Ic21-0506@lclari X =6104_EP#6 - Google Docs ent/d/104sNODGApfrUg768nDS9piPmOB-VzXs86b_t-tDcDF8/edit My Drive - Google... WebAdvisor Main... ols Extensions Zotero Help text T Arial Haplontic life cycle Diplontic life cycle Parthenogenesis Karyotype Histone - 11 + 2 4. Match the words with the correct definitions: | Alternation of generations Job Listing - USF... WT Enrollment Requir... B I U 3 4 X 0 Lecture Video: The Cell Cyc x Chemical Principl... UO UND Orn Z = - 1:3 A type of reproduction that produces a clone of the mother A protein DNA wraps around it to condense the DNA An organism alternates between 1n and 2n with each generation A complete set of chromosomes, as they appear in the nucleus When an organism spends most of its life in the 1n state When an organism spends most of its life in the 2n state 11
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