Suggest the qualitative test for the compound with molecular formula CeH;Br;0. Predict what information from IR and NMR spectra will help you to classify this compound.
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- 1. What is the concentration of the solution prepared by diluting 50 µl of ampicillin (1.0mg/ml) to a final volume of 1ml? 2. Determine the volumes of phenol, chloroform, and isoamyl alcohol required to prepare250 ml of a 25:24:1 (v/v) solution.Multiple choice: (use the picture as reference) Will this be soluble to CHCl3? * yes no Which part of the structure will react to Liebermann-Burchard test? * C17 -OH group Ring A Rings C and D Which of the statements is NOT TRUE about the sample in Salkowski test? Lower layer becomes red with a green fluorescence A molecule of cholesterol reacts with sulfuric acid to yield bi-cholestadien There should be an excess sulfuric acid to convert bi-cholestadien to bisulfonic acid of bi-cholestadien. It is best to shake the solution for a more accurate result.3. Discuss the advantage and disadvantage of Wijs method of iodine value determination. What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its basic principles and general methods involved.
- II. Heat of Neutralization. 3. Complete the table below. a) Initial temperature of hydrochloric acid 31.0 °C b) Initial temperature of sodium hydroxide 30.0 °C c) Average initial temperature of acid and base (°C) d) Final temperature of neutralized mixture 44.0 °C e) Change in temperature, AT (°C) f) qH20 for the reaction (J) (Assume 50.0 mL of solution and use average density of 1.02 g/mL) g) AHneut (J)Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol.5. n° of 2-chloro-2-methylpropane is 1.3850. If a 1g sample of 2-chloro-2-methylpropane was contaminated with 1 drop of ethanol, what do you think the effect would be on the refractive index and by approximately how much? (1 drop of ethanol weighs about 0.03 g).
- 3. The following absorbance spectrum was collected. What color is the sample solution? Explain using the color wheel. 1.4- 1.2 0.8 0.6+ 0.4 0.2 300 400 500 600 700 800 wavelength (nm) 4. Given the Beer's Law Plot for permanganate in the Introduction of the lab handout, what would be the concentration of an unknown permanganate sample if the absorbance was 0.308. Absorbance1.What is the molar concentration of a 15% glycerol solution? (Glycerol has a molecular weight of 92.1.)2.How do you prepare 750 mL of 0.5X gel running buffer from a 5X stock solution?1) If you eluted a TLC of a compound using 80% hexanes / 20% ethyl acetate as the solvent and obtained an R; value of 0.5. If you changed the solvent system to 50% hexanes / 50% ethyl acetate, how would the Rf value change? Explain why.
- 64. A skin test for fire ant allergy involves the intradermal skin prick of 0.05 mL of a 1:1,000,000 (w/v) dilution of fire ant ex- tract. How many micrograms of extract would be administered in this manner? 64. 0.05 µg fire ant extractMD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?Mention at least two simple chemical tests by which you can distinguish between benzaldehyde and benzoic acid.