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- You are analyzing the effluent from a lotion plant for parabens using HPLC. You have a working standard solution with a concentration of 59.4 ppm which returned a Peak Height of 10,164 and Peak Area of 5,315. You take 44.4 L of the water sample and concentrate it to 0.3 L and analyze this solution. The Peak Height is 9,119 and the Peak Area is 6,320. What is the concentration of parabens in the effluent of the plant in mg/L to two decimal places.How to prepare the solutions listed below Solution 1) 25 mL 7 M sulfuric acid and the respective dilution of this solution to prepare 50 mL 2 M sulfuric acid in deionised water. Sulfuric acid Mw = 98.079 g mol-1 Sulfuric acid density = 1.84 g mL-1 Sulfuric acid purity = 98% Solution 2) 100 mL 0.02M potassium permanganate potassium permanganate Mw = 158.03 g mol-1 potassium permanganate purity = 99%A sample of a soil was analysed for K+ as follows. A 2.50 g sample of soil was dissolved in acid and diluted to volume in 100 mL volumetric flask. A 10 mL of the resulting solution was transferred by pipet to a 50 mL volumetric flask and diluted to volume. An analysis showed that the concentration of K+ in the final solution was 9.81 ppm. What was the weight percent of K+ in the original soil?
- 3. The following mineral analysis was reported for a water sample taken from a local NJ well. Determine the total, carbonate and non-carbonate hardness in mg/L as CaCO3. Well No. 1, Lab No. 02694, November 9, 1971 Iron 0.2 Silica (SiO2) 20.0 Manganese 0.0 Fluoride 0.35 Ammonium 0.5 Boron 0.1 Sodium 4.7 Nitrate 0.0 Potassium 0.9 Chloride 4.5 Calcium 67.2 Sulfate 29.0 Magnesium 40.0 Alkalinity 284.0 as CACO3 Barium 0.5 pH 7.6 units Note: All reported as "mg/L as the ion" unless stated otherwise. Ca2 Alk NonSample 1 2 3 4 Sample Volume, mL 50.00 49.50 25.00 50.00 Ba(OH)2 Volume, mL 43.17 42.68 21.47 43.33 A 0.1475-M solution of Ba(OH)2 was used to titrate the acetic acid (60.05 g/mol) in a dilute aqueous solution. The above results were obtained. 1. Calculate the mean w/v percentage of acetic acid in the sample 2. Calculate the standard deviation for the results. 3. Calculate the 90% confidence interval for the mean. 4. At the 90% confidence level, could any of the results be discarded?A sample of an infusion was diluted 10 ml to 250 ml and then 10 ml to 200 ml. It was thenanalysed and was found to contain sodium at 0.789 mg/100 ml. Calculate the concentrationof sodium in the original sample in %w/v. The sample was composed of a mixture of sodiumlactate and sodium carbonate in equimolar amounts. Calculate the amount of sodiumlactate and sodium carbonate in mg/10 ml of the sample (Na = 23, lactate = 89, carbonate =60)
- 13. A fish meal sample has the following composition: protein 20%, moisture 12%, additive 66%. The sample is placed in a drying oven and subsequent analysis gave a value of 22% protein. Calculate the % moisture content of the dried sample. 14. 1.000 g of soil, as-received, gave a moisture content of 14.00%. The oven-dried sample, completely moisture-free, showed 18.00% K. Find the percentage of K in the sample as received.The standard addition method is used to analyze a sample of a river water for mercury. Solution A is made by pipetting 5.00 mL of undiluted sample in to a 10 mL volumetric flask and filling to the mark with D water. Solution B is made by pipetting 5.00 mL of undiluted sample and 3.00 mL of 15.0 ppb of Hg standard into same 10.0 mL volumetric flask and filling to the mark with DI. Solution A and B are analyzed using atomic absorption spectroscopy and give a percent transmittance values of 56 % and 33 % respectively (not blank corrected). A blank has a transmittance of 96%. Which of the following technique can be used to determine the amount of mercury in the undiluted sample of river water? O A. IR spectroscopy O B. Cold vapor atomic absorption OC. Hydride generation atomic absorption OD. Electrothermal atomic absorptionThe homogeneity of the chloride level in a water sample from a lake was tested by analyzing portions drawn from the top and from the bottom of the lake, with the following results in ppm CI: Тоp 26.30 26.43 26.28 26.19 Bottom 26.22 26.32 26.20 26.11 26.49 26.42 Determine if there is a significant difference (at 95% confidence level) between the chloride level in the top and bottom levels of the lake.
- The protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 5.00 mg/mL BSA stock solution. To these dilutions, 100 µl of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm. Standard # BSA, in mg/mL Volume of Bradford reagent, A595 in mL 1 5.0 0.000 1.00 5.0 0.134 3 2.00 5.0 0.265 4 3.00 5.0 0.388 4.00 5.0 0.497 The supernatant dilution was prepared by mixing 49 µL of the protein isolate with 16 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.413. Note: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of calculation.What are the types of gravimetric methods? Give an examples where each method is used in an analysis. What is particulate gravimetry? When do we use this technique?Dr. Caras was characterizing a cytochrome (an iron containing protein) from a new strain of bacteria. He obtained eight measurements of the percent iron in the protein: 0.4908%, 0.4902%, 0.4899%, 0.4899%, 0.5012%, 0.4891%, 0.4903%, and 0.4892%. Calculate the mean (x) and standard deviation (s) for these results. x = S= Use the Grubbs test to determine if one of these values is an outlier. What is the value of Gcalculated? Gcalculated = % %