Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD: RATIONALE:
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Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option?
METHOD:
RATIONALE:
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Solved in 2 steps
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateWhich physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperIdentify the method of sterilization/ chemical agent described This chemical agent is used as a standard for evaluating new chemical agents. This chemical agent is used as water disinfectant. A phenol-derivative that is less exotic and more potent than phenol. Considered the best antiseptic. Used as prophylaxis for ophthalmia neonatorum.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- Agar is a solidifying agent used in media preparation. How and why does the agar concentration in semisolid media differ from conventional solid media?Explain the different categories that determine when equipment must be disinfected or sterilized. Identify a common cleaning and disinfectant solution. Describe the recommended application and purpose for each solution.Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
- Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.In paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using x-ray crystallography and infrared spectroscopy? Please include the principles and the details and type specifically for the antimicrobial agents of the methodology.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?