Show the separation pattern of the following DNA molecules on 1 % agarose gel. Justify your results. 5 kbp 5 kbp 5 kbp 5 kb +
Q: Propose a reasonable biosynthesis for Compound 14 starting from Acetyl CoA, SAM, S- alanine, S-pheny...
A: Here compound 14 is synthesized from L-Phenylalanine in multistep process. The structure of all the ...
Q: In cation exchange chromatography,
A: here they are talking about the stationary phase of cation exchange chromatography. The stationary p...
Q: 1) What is the chemical group at the end of the R groups of basic amino acids that makes them neutra...
A: There are twenty standard amino acids that make up all of the proteins. The twenty standard amino ac...
Q: Match the following structural composition of each polysaccharide with its identity ...
A: Polysaccharides, also known as polycarbohydrates, are the most abundant carbohydrates found in food....
Q: In generating CO2, how can the citric acid cycle discriminate between the carbons in citrate given t...
A: In the first phase of the cycle, acetyl CoA combines with oxaloacetate, a four-carbon acceptor molec...
Q: Cleavage of a phosphate bond is needed for de-polymerization of which mature polymer? Neither microf...
A: The eukaryotic cytoskeleton is composed of microfilaments, microtubules, and intermediate filaments....
Q: . CTGATTCCG AATGS Given a part of DNA undergoing replication. Copy and write the corresponding bases...
A: DNA replication is a process by which each strand of DNA acts as a template and copies itself to pro...
Q: What are the main structural features of an amino acid?
A: Amino acids are compounds containing carbon, hydrogen, oxygen and nitrogen. They serves as building ...
Q: 3. Draw out the first 3 enzymatic reactions of the PPP, including listing names of S, P, coenzymes, ...
A: Hi! Since you have posted multiple questions and have not mentioned which to answer, we are answerin...
Q: Which statement(s) correctly describe(s) protein structures? A. All hydrophobic residues are buried ...
A: Proteins are made up of amino acids, which are the building blocks. Around 20 different amino acids ...
Q: unfold the protein
A: here they are talking about protein unfolding. The tension applied by the cantilever during protein ...
Q: How does protein form?
A: The Central dogma of molecular biology outlines the steps by which the information encoded in the DN...
Q: Based on the Agarose Gel Electrophoresis results, tell in which concentration range of KCl the restr...
A: Ion-exchange chromatography separates molecules based on their charge properties. Based on charge pr...
Q: elp ASAP, thank you! (question is asking to pick Which 6 features are shown in the figure?)
A: The reaction given in the figure shows the enzyme catalyzed reaction with the formation and breakage...
Q: 2. 1 Calculate the overall charge (pH 7) on the following three polypeptides and answer the question...
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which on...
Q: The protease used in this procedure functions best at 50°C. Would you expect this enzyme to be isola...
A: Microorganisms are classified as, psychrophiles, psychrotrophs, mesophiles, thermophiles, and hypert...
Q: A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown b...
A: The rate of enzyme catalyzed reactions is directly proportional to the substrate concentration. But,...
Q: Explain the enzymes.
A: Enzyme, a molecule that works as a catalyst in living organisms, regulating the pace at which chemic...
Q: Most of the ultraviolet absorption of proteins at 280 nm is due to their content of O Tryptophan O A...
A: Proteins in solution absorb UV light at 280 and 200 nm, exhibiting absorbance maxima at these wavele...
Q: What are the biomolecules that are used to biosynthesize a purine ring in the de novo pathway – prov...
A: Purine de novo synthesis is a time-consuming process. It happens actively in the liver cytosol, wher...
Q: Write the structure formula, three-letter and one-letter abbreviation for each essential amino acid ...
A: Amino acids contain amino group and carboxyl group along with R side chain. The R side chain defines...
Q: A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown b...
A: To determine the values of km and Vmax by lineae regression, we need to plot lb plot. LB plot can be...
Q: What are the main ingredients in the manufacture of mayonnaise? Explain the role of each component.
A: Mayonnaise is a food ingredient that is an emulsion of oil, egg yolk, acid and either vinegar or lem...
Q: Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in thi...
A: In biochemistry , a chemical test is performed in qualitative or quantitative procedure to identify ...
Q: Q24. Choose the best answer for the following questions.
A: Amino acids are building blocks of proteins and also important as a precursor for the biosynthesis o...
Q: _____________ is a tranquilizer that is also known as the “date rape” drug. a. Halcion b. Librium c....
A: Tranquilizer is a drug which is used to reduce anxiety and stress. Dopamine is a neurotransmitter wh...
Q: How does a protein form?
A: Proteins are the heteropolymers of twenty standard amino acids attached together via peptide bonds. ...
Q: What are nucleosides?
A: Nucleosides are compounds consisting of purine or pyrimidine bases linked to sugar. The nucleobase c...
Q: An enzyme catalyzes a reaction with a Km of 7.50 mM and a Vmax of 2.90 mM · s-!. Calculate the react...
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that o...
Q: What is the importance of central dogma
A: DNA contains genetic information in the form of nucleotide sequences. DNA is composed of four differ...
Q: Propose a reasonable biosynthesis for Compound 14. MUST BE in this order: Acetyl CoA SAM S-alanine S...
A: Here compound 14 is synthesized from L-Phenylalanine, SAM, L-alanine, and Acetyl-CoA in multistep p...
Q: How many amino acid units do the structure below has? HN. OH OH HO A.) 5 В.) 10 О с.) 9 D.) 8 E.) 7
A: Amini acids are the building blocks of proteins linked through the peptide bond (CO-NH). There are 2...
Q: An effort is usually made to purify a protein fırst, before its characteristics are determined. True...
A: Purification of a protein is done to get the interested protein in as native form it can be. Purific...
Q: Consider a protein with a beta conformation sequence EAGQVHRGP a) Which residue(s) can be substitute...
A: Beta conformation in protein: Beta sheet, beta-turn etc are beta conformation in protein. Beta sheet...
Q: What assay could be used to test the function of a protein in the glycoside hydrolase family?
A: Glycoside hydrolases are enzymes that catalyze the hydrolysis of the glycosidic linkage of glycoside...
Q: What are the complications of having a high blood glucose level?
A: Glucose is an essential nutrient for the growth and development of cells in most aerobic organisms. ...
Q: The molecule shown below is NH2 HO N. Но но
A: Deoxyribonucleic acid (DNA) is a molecule comprised of two polynucleotide chains that wrap around ea...
Q: 1 Active Site Reaction Mechanism Inhibitor HN- 2•Zn NH2 HO. HO, NH2 N N- NH `NH NH NH, N' 'N N 'N NH...
A: Hi! Thank you for the question, as per the honor code, we are allowed to answer the ...
Q: Are there any research involving white vinegar that it is effective as an ingredient in making insec...
A: Acetic acid is one of the most fundamental organic acids. In this context, "organic" refers to a che...
Q: Why does it make sense that under conditions of low ATP levels in the cell the pyruvate carboxylase ...
A: Glucose is converted to pyruvate through the process of glycolysis in the cytoplasm. The process of ...
Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size exclusio...
A: SDS-PAGE is biochemistry technique used to separate proteins based upon the relative mobility of cha...
Q: Show the relationship between lipids, nucleic acids, proteins, and carbohydrates including the conne...
A: A biomolecule, also called a biological molecule, is a chemical compound found in living organisms. ...
Q: Urease enzyme hydrolysed urea at [S]= 0.03 mmol/L with a Km value of around 0.06 mmol/L. The initial...
A: Vmax is the reaction's maximum speed at which all of the enzymes become saturated with the substrate...
Q: 1. Protein that will most strongly bind to an anion exchange column: [ Select] 2. Protein that will ...
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids conta...
Q: Derive the amino acid sequence that is coded for by each of the following mRNA sequence. 5' CAA GA...
A: Amino acid are molecules which are combine to form a protein molecules.Amino acid are synthesized by...
Q: Directionality of Polynucleotide chains originates at the ____ end, and terminates at the _____ en...
A:
Q: Pioglitazone, a drug used to treat diabetes, causes some membrane-embedded portions of mitochondrial...
A: Complex I is the largest multi-subunit complex of Mitochondrial Respiratory Chain (MRC), that causes...
Q: Double-helical DNA has major and minor grooves because
A: The question is regarding DNA double helix structure. The structure of a DNA molecule is described a...
Q: (a) Propose a biosynthetic pathway to the secondary metabolite, B. OH O HO Metabolite B
A: The given metabolite structure is derivative of Salicylaldehyde and synthesized by plant as secondar...
Q: What is the general definition of an uncoupler protein? In the context of oxidative phosphorylation,...
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids conta...
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
- Which well(A through E) of this agarose gel contains the smallest DNA size? Please look at the pic.To answer the prompts below, you will need to draw the chemical structure of the trinucleotide 5' - ACG - 3', labeling the 5' and 3' ends. Opposite this structure, draw the complementary trinucleotide to make a double-stranded DNA molecule. A. What is the complementary trinucleotide sequence from 5' to 3' (enter answer as e.g. CGA)? TCG B. How many non-covalent hydrogen bonds stabilize this structure? 8 C. How many covalent phosphate linkages stabilize this structure? 10 D. Which type of bond takes less energy to break? Phosphate linkage O Non-covalent hydrogen bondsPut the following pieces of DNA in order that they would appear reading from the top (nearest the loading point) to the bottom of a gel, with "first" meaning nearest the top. First [ Choqse [Choose] 21,000 basepairs (bp) Second 10,000 bp 23 kilobases (kB) 18 kB 2,300 bp Third [Choose ] Fourth [ Choose] [Choose] Fifth > >
- Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.What is the role of GelRed® in Agarose gel electrophoresis of DNA fragments? Please select the single answer that is most correct GelRed® moves down the agarose gel in response to the electric current and enables visualisation of the position of A the nucleic acids within in the agarose gel. GelRed® intercalates with the Nucleic acid and, under UV light, fluoresces to enable visualisation of the position of the nucleic acids in the agarose gel. GelRed® intercalates with the Nucleic acid and enables visualisation of the position of the nucleic acids in C the agarose gel. GelRed® intercalates with the amino acids in the agarose gel and enables visualisation of the position of their in D the agarose gel.Make your DNA extraction buffer. Add 2 teaspoons of detergent, 1 teaspoon of salt, and ½ cup of water to one of the plastic cups. Stir well. 4 Open the bag of fruit and add 2 teaspoons of your DNA extraction buffer. What are cell membranes made of? Which parts are hydrophobic and which are hydrophilic? What does soap do to cell membranes? Why would this be helpful in extracting DNA? What function does the salt perform in the solution?
- Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…You extract DNA from 200, You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260) of the solution in the cuvette is 0.2 (Remember: an A 260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below What to Calculate Calculation / Result Marks (x / 100) 12/100 DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette 12/100 12/100 Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ 24/100 assuming an extraction efficiency of 40%A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What is it about the chemistry of DNA that causes it to be uniformly negatively charged?
- A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What if you had two different DNA fragments that were exactly the same length as measured in base-pairs. Would it be possible to distinguish them using this type of electrophoresis? How would they appear on a gel?During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityWhat size DNA fragment would be released after very mild digestion of chromatin with micrococcal nuclease? (This would represent a single cut per nucleosome.) 30 bp O 165 bp 146 bp 200 bp