Select all that would be true if I had a nonsense mutation in an exon of a gene: The nonsense mutant allele would be the same size as wildtype by PCR-electrophoresis The nonsense mutant protein would be the same size by Western as the wildtype protein The nonsense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The nonsense mutant protein would be a different size by Western compared to the wildtype protein
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- Select all that would be true if I had a missense mutation in an gene: The missense mutant protein would be the same size by Western as the wildtype protein The missense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The missense mutant protein would be a different size by Western compared to the wildtype protein The missense mutant allele would be the same size as wildtype by PCR-electrophoresisFor separating DNA of different sizes, you would use Question PCR Questi Gel electrophoresis Restriction enzymes 1 Listen Crispr For making specific DNA changes in living organisms, you would use ** Crispr Restriction enzymes Gel electrophoresis PCR ◄0 Listen You are interested in identifying genes that determine tail length in dachsunds. You analyze the genome of several dachsunds and look for SNPS that correlate with tail length. The results are shown here pog Spot Rover Chase Frisbee Cin Checkers SNP 1 SNP 2 SNP 3 Tail Length (cm) 10 12 SNP 4 9 4 3 10 SNP 1 C C C G C SNP 2 A T T A A T Based on these results which SNP is best correlated with tail length SNP 3 C C C G C SNP 4 G G T T T GSTEP BY STEP Exaplanation would be appreciated so I can understand for upcomming exam. In humans, the COL1A1 locus codes for a collagen protein found in bone. A recessive COLA1 allele believed to reduce bone density and increase risk of fractures differs from the wild type allele by the presence a GT in the first intron of the gene. This mutation can be easily screened by PCR, using COL1A1-specific primers followed restriction enzyme digest of the product with MscI. The normal allele is denoted as G and the recessive allele as T. A recent study of 894 women found that 570 were GG, 291 were GT and 33 were TT. (Assume for this exercise that these values reflect both male and female values for the sampled population. What are the frequencies of each allele? What are the frequencies of each genotype? c. Is this locus in Hardy-Weinberg equilibrium? Use the table below to evaluate the significance of any deviation from H-W expectations.
- Here is a DNA agarose gel showing PCR products from a mouse genotyping experiment. Genotyping tells us whether each mouse is a wild type mouse (i.e. not genetically modified) or a mutant mouse. Interpret the results for each mouse 1-3.The temperature at which the primers and target DNA hybridize may be changed to influence the stringency of PCR amplification. What effect will changing the hybridization temperature have on the amplification? Let's say you have a certain yeast gene A and want to check whether it has a human equivalent. How might managing the hybridization's rigor benefit you?From your knowledge about DNA microarray, answer the following: A- How DNA microarray is created? and why it is referred to as “hybridization technology”? B- Why RT-PCR is important in the sample preparation to perform expression microarray experiment? C- Mention the name and the color of the dyes used in expression microarray? D- If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?
- The sequence below shows 140bp of a double-stranded DNA sequence coding for histone H2A. What two primers could be used to amplify the whole 140bp sequence in a PCR reaction? 1 70ATGTCTGGACGTGGCAAGCAGGGAGGCAAAGCCCGCGCTAAGGCCAAGACTCGCTCTTCTAGGGCCGGTCTACAGACCTGCACCGTTCGTCCCTCCGTTTCGGGCGCGATTCCGGTTCTGAGCGAGAAGATCCCGGCCAG 71 140 TCCAGTTCCCCGTGGGCCGAGTGCACCGCCTGCTCCGCAAAGGCAACTATGCCGAGCGGGTCGGGGCCGGAGGTCAAGGGGCACCCGGCTCACGTGGCGGACGAGGCGTTTCCGTTGATACGGCTCGCCCAGCCCCGGCC a. TACAGACCTGCACCGTTC and GGCCGGGGCTGGGCGAGC b. ATGTCTGGACGTGGCAAG and CCGGCCCCGACCCGCTCG c. ATGTCTGGACGTGGCAAG and GCTCGCCCAGCCCCGGCC d. TACAGACCTGCACCGTTC and CGAGCGGGTCGGGGCCGGAbove what amplicon size is this PCR reaction dNTP limited? The reaction contains 35 pmoles of each 24 base primer, 1.2 mM dNTP's and is 40 uL in volume. Answer in base pairs, but don't include the unit in your answerThe image below shows the general structure of a gene on a chromosome. The arrows above and below the chromosome indicate the binding positions of potential forward (F) and reverse (R) PCR primers. Select two primers from the list below that would exclusively amplify exon 3 in a PCR reaction. ut of Intron 1 Intron 2 +1 Poly-A signal ATG TAG F1 Exon 1 F4 Exon 2 F6 Exon 3 R6 R4 R3 R2 R1 Promoter Select all that apply: cross out Da. F1 cross out Ob. F2 cross out Oc. F3 cross out O d. F4 cross out O e. F5 cross out f. F6 cross out g. R1 cross out Oh. R2 cross out OL R3 cross out O. R4 cross out k. R5 cross out R6 TI
- The short sequence shown below is part of a gene of interest. You decide to amplify this part of the gene by PCR. One primer has been provided by your lab supervisor as shown 3 GAGAGC 51 5'TGAGCAGCGATGCCGATGTTGCCAATGCAGCTCTCG-3'| Which of following additional primers would you need to make? OA S'ACTGCT'3 OB. S'ACGAGT3 OC. SUGAGCA'S D. STGAGCA'3For this activity you will draw out the first steps of the PCR reaction, using the following as a short template. Take a picture of your drawing and upload it to this Question. 5' TTGCGTACGTGCATGTGTGCACATATGTCC 3' 3' AACGCATGCACGTACACACGTGTATACAGG 5° In your upload submission be sure to include: 1. Label the 5' and 3' end of the template and each primer, and indicate the direction in which polymerization will take place. 2. Write the sequence of a 10 base pair Forward primer and a 10 base pair Reverse primer binding to the template strand. Add an arrow showing which direction polymerization will take place 3. Add a DNA polymerase to the drawing above showing where the polymerase will bind. 3. Draw and label a DNTP nucleotide. Show how this monomer gets incorporated into a new DNA strand?After running a qPCR experiment, we will have graphs showing the amount of fluorescence detected by the digital camera compared to the number of PCR cycles run. Suppose you see the following graph output by the qPCR machine: Relative Fluoresence 3.0 2.5 2.0 1.5 1.0 0.5 0.0 0 10 20 30 40 50 Cycles Which curve (blue, red, or green) represents a sample with the smallest amount of mRNA present? Why? Be sure to discuss Ct values in your answer.