Running an HPLC assay using a column heated to approximately 60 °C can have what benefits over running the assay room temperature? Select all that are true. increased column life decreased run time greatly enhanced peak shape ✔more precise quantitation Oflucuating retention times O increased back pressure more precise retention times Submit Answer Incorrect. Tries 1/4 Previous Tries

Running an HPLC assay using a column heated to approximately 60 °C can have what benefits over running the assay room temperature? Select all that are true. increased column life decreased run time greatly enhanced peak shape ✔more precise quantitation Oflucuating retention times O increased back pressure more precise retention times Submit Answer Incorrect. Tries 1/4 Previous Tries

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter3: Statistical Tests With Excel

Section: Chapter Questions

Problem 9P

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A sample of explosives residue is dissolved in an appropriate solvent and is injected into HPLC. The column contains a non- polar stationary phase. The sample revealed three chemical compounds that are commonly present in homemade explosives. ТAТP DADP HMTD 1 2 3 4 5 6 7 8 Retention time (minutes) a) determine the retention time of TATP. b) which one elute first :DADP,TATP or HMTD? c) State and explain which compound is most polar. d) If you are investigating this terrorist attacks, state one way in which the information in the chromatogram would be useful.
Answer sections C, D and E in the same answer book. Section D (Chromatography) Question 8 The following data were obtained for the analysis of a mixture of compounds (A-D) by HPLC: Peak Retention time (min) | Width of peak base (min) non-retained 2.1 A 6.4 10.51 B 11.3 1.03 C 13.1 1.25 D 21.6 1.62 a) Using peak C. calculate the number of plates (N) in this column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C, calculate the resolution (R).
Determine the Kovats retention index for an unknown C9 sample using the retention times 1.3 min for benzene, 16.4 min for p-xylene, 18.7 min for the unknown C9 sample, and 20.4 min for p-divinyl benzene. Question 1 options: 864.862 846.801 892.106 920.663
In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller molecules
A solute has a retention time of 5.4 min with a width at half height of 0.41 min on a column 12.2 m long. Find the number of plates. Please round-off your answer to a whole number (no decimal place)
Compare the quantities For a normal phase chromatographic technique: KA > KB Choose A if Polarity of A > Polarity of B B if Polarity of A< Polarity of B C if % both is equal D Cannot Be Determined
The internal standard method compensates for errors that affect both the analyte and the reference. True False
A GC-MS was used to analyze an unknown sample. Based off the chromatogram, at least how many different compounds were in this sample? Detector Signal u M Retention Time
A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
Question 2 Retention time is the time spent by analyte in both mobile and stationary phases. Co A) True В False
If the peak width w of both Peak 1 and Peak 2 fro eon 1g is 0.5 minutes, what is the resolution factor between the two peaks? 1.13 Selectivity is Peak 1 (6.0 min, Peak 2 w=0.5 (6.8 min, w=0.5 25 min) min) 20 15 Hold up peak (1.8 min) 10 0. 0. 4. 8. time (minutes) 2. 5. Signal
In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.