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- In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.A nurse who created a sterile field for a patient is adding asterile solution to the field. What is an appropriate actionwhen performing this task?a. Place the bottle cap on the table with the edges down.b. Hold the bottle inside the edge of the sterile field.c. Hold the bottle with the label side opposite the palm ofthe hand.d. Pour the solution from a height of 4 to 6 inches (10 to15 cm).
- A nurse who created a sterile field for a patient is adding a sterile solution to the field. What is an appropriate action when performing this task? a. Place the bottle cap on the table with the edges down. b. Hold the bottle inside the edge of the sterile field. c. Hold the bottle with the label side opposite the palm of the hand. d. Pour the solution from a height of 4 to 6 inches (10 to 15 cm).What type of medium is best used to maintain a preserve specimen before clinical analysis? A. Enriched b. Selective c. Differential d. Reducing e. NutrientFor each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E System for Enterobacteriaceae identification. a. time requirement b. specimen preparation c. tests utilized d. anaerobic conditions e. interpretation of results
- Make illustrated diagrams of stepwise procedures for the performance of the following tasks. 1. Sterilization of Petri dishesIn the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubesChoose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
- write the procedure for the following a. celloiding embedding b. epoxy embedding c. vacuum embeddingTo retrieve a sample from a culture tube with an inoculating loop, the cap of the tube is a. removed and held in one’s teeth. b. removed and held with the fingers of the loop hand. c. removed with the fingers of the loop hand and placed in the fingers of the tube hand. d. removed with the fingers of the loop hand and placed on the laboratory bench. e. Any of these methods can be used.Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube