Retention factors for three solutes separated on a C8 nonpolar stationary phase at pH = 3 are given. Eluant was a 70:30 vol/vol mixture of 50 mM citrate buffer and methanol. a)Draw the dominant chemical structure of each of the substances at pH = 3. b)The retention factor for acetophenone is 4.21, for salicylic acid is 2.97, and for nicotine is zero. Explain the different retention factors.
Retention factors for three solutes separated on a C8 nonpolar stationary phase at pH = 3 are given. Eluant was a 70:30 vol/vol mixture of 50 mM citrate buffer and methanol. a)Draw the dominant chemical structure of each of the substances at pH = 3. b)The retention factor for acetophenone is 4.21, for salicylic acid is 2.97, and for nicotine is zero. Explain the different retention factors.
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter23: Carbon: Not Just Another Element
Section: Chapter Questions
Problem 52PS
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5) Retention factors for three solutes separated on a C8 nonpolar stationary phase at pH = 3 are given. Eluant was a 70:30 vol/vol mixture of 50 mM citrate buffer and methanol.
a)Draw the dominant chemical structure of each of the substances at pH = 3.
b)The retention factor for acetophenone is 4.21, for salicylic acid is 2.97, and for nicotine is zero. Explain the different retention factors.
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