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Refer to the chromatograph (Figure 1) below and answer the questions that follow:
- What is the volumetric flow rate of the column?
- What type of compound is likely to be found in peak B? Explain.
- What possible buffer changes could the researcher have applied at the 30ml mark? Explain.
- How would one avoid cross-contamination when using this type of purification
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- 50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is required? Explain the role of surface sterilising chemicals in aseptic culture initiation and function of sucrose in MS medium?15.5 R Erythromycin Lactobio- nate Dexamethasone Sodium Phosphate Glycerin 500 mg 100 mg 2.5 mL Sterile Water for Injec- tion ad M. ft. Ophthalmic Solution 100 mL (a) What is the percentage strength of erythromycin lactobionate in the prescription? (b) If glycerin has a specific gravity of 1.25, what is its concentration (w/v) in the prescription? 15. (a) 0.5% w/v erythromycin lactobionate (b) 3.125% w/v glycerinAcetaminophen Elixir 120 mg/5 mL During the Covid-19 epidemic, the public were advised high fever was a common side effect of infection and to ensure they had acetaminophen on hand. Because of this, there was a sudden nation-wide shortage of commercial liquid acetaminophen. The local Doctor asked if you could compound a mixture for a young child with otitis media as the Mother cannot find any commercially. The Pharmacist did some research and found the following referenced formula for you to use. The Doctor would like to give 50 mL. The child weighs 16 kg. Formulation: Acetaminophen 120 mg 90% ethanol 0.7 mL Propylene glycol 0.5 mL Purified water 0.5 mL Glycerol qs ad 5 mL Dissolve acetaminophen in ethanol and propylene glycol, add most of the glycerol, add water, adjust to volume with glycerol. Strength: 120 mg/5 mL Child dose: 15 mg/kg q4-6h. Maximum 4 doses in 24 hours. Warnings: Contains 13% alcohol EUD: As per local guidelines. Questions: Calculate appropriate…
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingApple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).
- The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following directions. Add 8 mL of diluent to yield 10mL of reconstituted solution. How many mL will you give?Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.22-A. Consider a chromatography experiment in which two compo- nents with retention factors k, = 4.00 and k2 = 5.00 are injected into a column with N = 1.00 × 10° theoretical plates. The retention time for the less-retained component is t,1 = 10.0 min. (a) Calculate fm and 1,2. Find w12 (width at half-height) and w (width at the base) for each peak. (b) Using graph paper, sketch the chromatogram analogous to Figure 22-7, supposing that the two peaks have the same amplitude (height). Draw the half-widths accurately.
- Streptomycin sulfate 400 mg IM daily is ordered for a child weighing 77lbs. The recommended dosage is 20-40 mg/kg/day once daily. A 1 gram vial of streptomycin sulfate is available in powdered form with the following instructions: Dilution with 1.8 mL of sterile water will yield 400mg per mL. If safe, how many mLs will you give?AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?1:55 .ll 0.52 KB/S 56 1.pdf CHEM 3141: BioChemistry Quiz no. 1- Answer key I.) Draw the following compounds. 1.) Maltose: 2 Glucose, a-(1,4) 2.) Gentobiose: 2 Glucose, B-(1,6) 3.) C-2 epimer of Talose 4.) C-3 epimer of Fructose 5.) C-2 epimer of ribose II. Predict the products of the following reactions, if there is none, write NO RXN. Also indicate, if the reaction is fast or slow он FO HO -H H -OH но. + Resorcinol/dilute HCI 1.) 2.) Sucrose + Copper (II) Acetate/Acetic Acid HO H HO H HO H H OH 3.) HO + Orcinol/HCI