Question 9 5 pts 95 58 40 HS SH HS 8 M urea and 65 72 B-mercaptoethanol 65 26 Hs84 Hs HS HS 110-SH 84 110 72 26 95 124 40 58 Native ribonuclease Denatured reduced ribonuclease Figure 2.53 Biochemistry, Seventh Edition O 2012 W. H. Freeman and Company The image above shows how Anfinsen unfolded ribonuclease for his classic experiment to see if proteins contain all necessary information for adopting their folded states on their own. To achieve proper refolding and ribonuclease activity, Anfinsen must have: trick question: ribonuclease cannot adopt its folded state without the help of cellular machinery removed the mercaptoethanol followed by the urea very carefully removed both the urea and mercaptoethanol at the same time removed the urea followed by the mercaptoethanol

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
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5 pts
Question 9
95
58
40
HS
HS
SH
65
8 M urea and
72
B-mercaptoethanol
65
Hs84 Hs-
SH
72
26
HS
110 SH
84
26
110
95
124
40
58
Native ribonuclease
Denatured reduced ribonuclease
Figure 2.53
Biochemistry, Seventh Edition
O 2012 W. H. Freeman and Company
The image above shows how Anfinsen unfolded ribonuclease for his classic
experiment to see if proteins contain all necessary information for adopting
their folded states on their own. To achieve proper refolding and
ribonuclease activity, Anfinsen must have:
trick question: ribonuclease cannot adopt its folded state without the help of
cellular machinery
removed the mercaptoethanol followed by the urea
very carefully removed both the urea and mercaptoethanol at the same time
removed the urea followed by the mercaptoethanol
Transcribed Image Text:5 pts Question 9 95 58 40 HS HS SH 65 8 M urea and 72 B-mercaptoethanol 65 Hs84 Hs- SH 72 26 HS 110 SH 84 26 110 95 124 40 58 Native ribonuclease Denatured reduced ribonuclease Figure 2.53 Biochemistry, Seventh Edition O 2012 W. H. Freeman and Company The image above shows how Anfinsen unfolded ribonuclease for his classic experiment to see if proteins contain all necessary information for adopting their folded states on their own. To achieve proper refolding and ribonuclease activity, Anfinsen must have: trick question: ribonuclease cannot adopt its folded state without the help of cellular machinery removed the mercaptoethanol followed by the urea very carefully removed both the urea and mercaptoethanol at the same time removed the urea followed by the mercaptoethanol
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