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QUESTION 7 "ou are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosz) in a Pseudomonas sample treated under various com e-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sa untreated sample? nosz Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) experimental gene Ct values for treatment sample experimental treatment sample Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher O B. -100 times higher O C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control

QUESTION 7 "ou are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosz) in a Pseudomonas sample treated under various com e-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sa untreated sample? nosz Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) experimental gene Ct values for treatment sample experimental treatment sample Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher O B. -100 times higher O C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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QUESTION 7 You are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosZ) in a Pseudomonas sample treated under various conditions. Using the 2-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sample verses the untreated sample? nosZ Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) gene Ct values for treatment sample experimental treatment sample experimental Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher B. -100 times higher C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control
Transcribed Image Text:QUESTION 7 You are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosZ) in a Pseudomonas sample treated under various conditions. Using the 2-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sample verses the untreated sample? nosZ Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) gene Ct values for treatment sample experimental treatment sample experimental Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher B. -100 times higher C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control
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