Question 1 During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification Question 2
Q: Why are cell membranes disrupted by soap?
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Q: Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
A: Hydrolysis of DNA will cause the DNA backbone to break down and ultimately give the deoxyribose…
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A: Double-stranded DNA is the most common kind of DNA molecule found in nature. The strands can be…
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A: An instrument called spectrophotometer is able to measure the absorbance of specific wavelength of…
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Q: Please answer the following using the experiment data below. Provide the formulas or methods used…
A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.
Q: di-deoxy nucleotides terminate DNA elongation in Maxam-gilbert method. True False
A: First-generation DNA sequencing methods include Maxam–Gilbert sequencing and the Sanger method.…
Q: Can you please check my answer and make sure it is correct. Question: Describe the two roles…
A: Primers provide specificity for amplification of the target sequence. They are typically 18-30…
Q: The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily…
A: How size determine the rate of migration:- Smaller DNA molecules migrate Faster through the gel.…
Q: What are the roles of the following reagents in DNA extraction? a. Ethanol b. NaCl c. SDS d. TE…
A: A) The initial role of the ethanol and monovalent cations is to remove the solvation shell…
Q: Question 1. Restriction endonucleases can be isolated from a number of bacteria. In bacteria…
A: Restriction endonucleases are DNA cutting enzymes. They cleave the DNA at or near specific sequence…
Q: During nucleic acid hybridization, the probe is labelled O for DNA stability O to increase…
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Q: True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments…
A: During DNA isolation 95% ethanol is used for DNA precipitation not for DNA digestion and while we…
Q: All of the following are examples of materials that are bound by your purifying medium during the…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Loading dye stains the DNA molecules and thus help to visualize it under UV light apparatus. O True…
A: DNA Loading dye is used to monitor the migration of DNA into the gel during electrophoresis.…
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A: The recombinant DNA technology (cloning) is used for the production of insulin, growth hormone (GH),…
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A: In this question, we have to complete the table for the reaction mixture of a PCR experiment.
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A: DNA is the nucleic acid and it absorbs the UV light with maximum absorbance at 260 nm wavelength due…
Q: What is the difference between SDS-PAGE and 2D electrophoresis
A: SDS-PAGE AND 2D electrophoresis- Both are isoelectric focusing mean also known simply as electro…
Q: Compare and contrast the running buffers for DNA and protein electrophoresis.
A: Electrophoresis- Electrophoresis is a method that separates macromolecules-either nucleic acid or…
Q: QUESTION 7 Primers are needed to start a PCR reaction True O False QUESTION 8 Restriction enzymes…
A: Introduction:- Restriction enzyme is a protein that recognise a specific, short nucleotide sequence…
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A: There are different molecular biology tools and techniques used to analyze and quantify…
Q: Following is the DNA repair mechanism for double stranded DNA damage: Question 33 options: Base…
A: Any of numerous ways by which a cell maintains the integrity of its genetic code is known as DNA…
Q: QUESTION 4 Which of these is not a component of the plasmid cloning vector O a. tetR O b. lacZ gene…
A: A vector is a tool to replicate foreign DNA into a host organism with the help of recombinant DNA…
Q: QUESTION 1 Match the discovery with the individual or groups that discovered it. (names may be used…
A: Introduction DNA:- It is a long molecule that contains our unique genetic code, It transmits the…
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A: Introduction DNA is crucial biomolecule which serves as genetic material in most of the living…
Q: During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Statement 1: Gel electrophoresis separates macromolecules like DNA and proteins according to the…
A: A molecular technique commonly used for the separation of proteins based on mass is called SDS-PAGE…
Q: Question 3 Based on the animation. DNA is a right handed double helix. O True O False
A: Deoxyribonucleic acid is a molecule consist of nucleotide in which contain sugar, phosphate group…
Q: What do you mean by amphoteric substance? Give examples of substance that is/are amphoteric. What…
A: Biochemistry is the study of biochemical functions at the cellular and molecular level using…
Q: Question one: PCR You want to amplify the underlined sequer 1) Design Forward and Reverse primers 2)…
A: PCR in Polymerase Chain Reaction which is used for the amplification of a given molecule of DNA.
Q: Question 5 Match the following components with the biotechnology technique they play a role in.…
A: Recombinant DNA technology is a technique by which foreign DNA is introduced into the host. DNA…
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Q: 22.124 Give two reasons why bacterial cells are used for recombinant DNA procedures. 22.125 What…
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A: DPPH free radical scavenging assay is a fast, basic, reasonable and broadly utilized technique to…
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Q: DNA EXTRACTION Materials: knife 1…
A: DNA extraction: It is the method used for the plants and animals both. It is the most recent and…
Q: QUESTION 3 Look at the following piece of DNA cut with enzymes I and II, crating 3 fragments; A, B…
A: Agarose gel electrophoresis isca technique used to seperate the DNA molecule based on their…
Q: These questions relates to DNA extraction, the choices are in the attached photos (i) Sanger…
A: DNA is the genetic material present in most of the living organisms. The DNA is made up of 4…
Q: Based on the quantitation data, please indicate how you would dilute the following samples. Fill in…
A: DNA shows maximum absorbance at the wavelength of 260 nm. Hence the concentration of DNA in solution…
Q: What part of the PCI Method of DNA extraction explains the: Cell Lysis Separation Precipitation
A: PCI DNA extraction method uses phenol, chloroform, and isoamyl alcohol as three basic ingredients to…
Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS) O…
A: Introduction DNA extraction is a method to isolate DNA in a biological sample, which is done by…
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A: * Salt is used in process of DNA extraction because salt it will neutralizing DNA that makes them…
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A: There are different techniques to study DNA and gene present in it.
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- patibility Mode] UE Layout References Mailings Review View A A Aa vA 前、一间刷 一| T AaBbCcDdE AaBE x A Av X, Normal No Spacing Headi co Edit and Save Start your free one month trial of Microsoft 365 or sign in to activate an existing subscription. 9. In human beings, the gene for red-green colorblindness (r) is sex-linked and recessive to its allele for normal vision (R), while the gene for freckles (F) is autosomal and dominant over its allele for nonfreckled (f). A nonfreckled, normal-visioned woman whose father was freckled and colorblind, marries a freckled, colorblind man whose mother was nonfreckled. a. What is the genotype of the woman's father? b. What is the probability that the couple's first child will be a non-freckled, normal visioned girl c. What is the probability that the first two children born to the couple will be freckled and colorblind girls? d. What is the probability that the first child born to the couple will either be a freckled, colorblind boy or a non-freckled,…Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…Search (Option + Q) Review View Help O Editing v A BIU D Av A ... Question 4 The use of physical or chemical nonviral transfer of genetic material is called Transfection Transduction Transformation Trangenesis Question 5 Congenital hearing loss is mostly autosomal recessive. When two persons of congenital hearing loss marry, as they often do, their biological children have normal hearing. The reason for the children to have normal hearing is that one of the parents is homozygous recessive for one gene and the other parent is homozygous recessive for another gene all the normal children have reverse mutations in their DNA each of the parents has reverse mutations in the germline cells early in their life. all normal children are homozygous recessive. Question 6 The enzyme responsible for transcribing complementary DNA from MRNA is DNA Polymerase Reverse transcriptase RNA Polymerase Endonuclease
- Answer the following: 1. Which pieces of DNA are the most informative? Why? 2. Explore the concept of "depth of coverage" (the number of fragments that cover a particular span of the contig). Where is the greatest depth of coverage? Where is the least depth of coverage? 3. What do the patterned bands represent? 4. Was it easier to assemble fragments that had multiple types of markers vs. just one type? Why? Assembling contigs out of DNA sequences (strings of As, Cs, Gs, and Ts) follows the same principle: instead of using markers, you line up fragments by overlapping DNA sequences.Titled “Techniques utilized to generate a genetically engineered organism and confirm gene disruption.” a. In the table, indicate with an "x" if the reagent or technique applies to DNA, RNA, and/or protein. You can have more than one "x" in each row or none at all.Please I want the answer of (B) and (C) Task 1. Your graduate advisor asks you to amplify the following sequence of DNA by PCR: 5’-ATACGCATTCGGACCAGGTCCTAA-3’ 3’-TATGCGTAAGCCTGGTCCAGGATT-5’ a. To ensure that the entire sequence above is amplified, what 6-nucleotide DNA primers should you add to your PCR mix? You order the primers listed above, but instead receive the following set of primers: 5’-CGCATT-3’ 5’-GGACCT-3’ b. What portion of the double stranded DNA molecule will be amplified after 10 rounds of PCR? Your labmate attempts to rescue your PCR reaction by providing you with the following set of primers: 5’-ATACGC-3’ 5’-TCCTAA-3’ c. What is the result of running the PCR reaction with your labmate’s primers? How many double stranded molecules of DNA will result from 10 rounds of amplification?
- QUESTION: There is an RFLP pattern that belongs to a disease. I need to find an inheritance pattern and marker related to the disease..Give only typing answer with explanation and conclusion Information: 1_Green Fluorescent Protein 2_nucleotide sequence, Amino acid sequence, and primers are obtained. 3_PCR protocol already described 4_bp has been calculations and estimated agarose gel image already designed. Questions: How do you analyze whether your target protein is expressed by E. coli cells. Explain your analysis method in detail and give information about the results you expect (in detail please)I need help finding the correct answers. the formula I used is % idenity=(length of the aligned region- the number of mismatches)/length of the aligned regionx100 for sequnce `1 and 3 I got a 70 percent idenity and 3 mismatches I think I am counting the wrong mismatches? can someone correct my answers and show me how they got theres and do not use chegg
- Are you a hidden heterozygote? A PCR analysis (part2) Agarose gel electrophoresis and interpretation la: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through the agarose gel. Briefly explain how each of these factors affects DNA migration. Agarose gel concentration: Time: Voltage: 1b: Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer. 1c: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? 1d: If the student is homozygous for the brown allele, how many bands will they see in the lanes for the blue and brown allele samples? (circle one) Brown sample: 0 Blue sample: 1 2 more than two. 1 2 more than two. le: If the student is homozygous for the blue allele, how many bands will they see in the lanes for the blue and brown allele…DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?