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- Result nad Discussion Lead Acetate Reaction: Samples: lysine, cysteine, methionine Reagents: 10% Sodium Hydroxide (NaOH) and Lead Acetate Pb(CH3COO)2 -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (40%) and boil the contents for 5-10 mins over a bunsen burner. Cool the contents and add few drops of 10% Lead acetate solution and observe.CARBOHYDRATE REACTIONS Five carbohydrate samples were analyzed for their qualitative reactions and the results are summarized in Table 1 below. Match the reaction profile of each sample to their possible identity from the choices. Table 1. Qualitative Tests of Carbohydrate Samples Lugol's Test Brown color Benedict's Test Rusty precipitates Aqua blue color Rusty precipitates Aqua blue color Rusty precipitates Sample No. Barfoed's Test Seliwanoff's Test Rusty precipitates Aqua blue color Rusty precipitates Aqua blue color Aqua blue color Pale yellow solution Pale yellow solution Cherry red solution Pale yellow solution Pale yellow solution 2 Brown color Brown color 4 Blue-brown color Brown colorNational Board of Medical Examiners Biochemistry Mark 36. In the presence of a metabolite (X), 6-phosphofructokinase is assayed at a fixed concentration of ATP and varying concentrations of fructose 6-phosphate. The resulting data are shown in the table. Fructose 6-phosphate (pM) 5 10 20 40 75 100 200 Velocity umoles/min 0.05 0.15 0.25 0.70 1.7 2.2 3.1 3.1 Velocity (+X) umoles/min 0.006 0.025 0. 10 0.35 1.03 16 2.9 3.1 400 Metabolite (X) is most likely which of the following substances? O A) ADP O B) AMP OC) CAMP D) Citric acid O E) Fructose-2,6-bisphosphate
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Fehling’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)Test 3. You are given testing results for the following nutritional drinks. What do you conclude about their contents? Reference the last lab for carbohydrate results. Unknown drink #1 lodine yellow Unknown drink #2 dark Benedicts Sudan blue green Unknown drink #3 yellow red ro no reaction 5% of tube red no reaction Biuret purple No reaction membrane purple Conclusion - What information do these results tell you about this drink? 14Test Results + or -? Points awarded Code Glu – acidic end products yellow ______ _____+ Glu – gas producedno ___-__ _____+ Lys – lysine decarboxylase yellow ______ _____ = _____ Orn – ornithine decarboxylaseyellow ______ _____+ H2S – iron sulfide producedno brown ______ _____+ Ind – indole produced beige ______ _____ = _____ Adon – acidic end products red ______ _____+ Lac – acidic end products red ______ _____+ Arab – acidic end products yellow ______ _____ = _____ Sorb – acidic end productsyellow ______ _____+ VP not needed Dul – dulcitol fermented green ______ _____ = _____ PA – deamination forms precipitate no brown ______ _____+ Urea – ammonia produced yellow ______ _____+ Cit – citrate catabolized green ______ _____ = _____ The V-P test in chamber 9 is an optional test to be used in case two species had identical results. It is not included in determining the 5-digit code. ` Final 5-digit code for the results: _____ _____ _____ _____ _____
- QUESTION 3 An unknown protein was determined using the following method: Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NaOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the following test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.8ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0.1ml Unknown 0.2ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2.…https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Seliwanoff’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)6 item / matching type Samples subjected to a test were given. Determine the objective of the test. Choices: Confirm the hydrogenation reaction Identify degree of unsaturation Determine the acid value. Differentiate fixed oil from essential oils Confirms the esterification of the hydroxyl group Test the presence of unsaturated FA Determine alkaline hydrolysis to produce salts of fatty acids Identify the nature of salt Confirm the presence of glycerol Methyl salicylate in Spot Test Beeswax in Saponification Test Linseed oil in Iodine Test Tallow in Acrolein Test Perla in Formation of Soluble Salt Cholesterol in Salkowski Test
- So we did an experiment and I am curious on the following because the prof did not elaborate too much. She just gave the postive result and reagent. 1. What type of reaction occurred when the samples (enumerated) reacted with the Bial’s reagent? Give the chemical equation for each.a. Glucoseb. Sucrosec. Fructosed. Lactosee. Galactosef. Ribose 2. Explain the mechanism behind the test in simple terms. 3. What are the important products of the reaction of each sample?Part 1: Assess the following partial results section below by editing it for brevity by omitting any unnecessary parts (1 point), explain why you decided to remove certain sections (1 point): To evaluate inhibitory effects of the selected molecules, 10mM stock solutions of each molecule were prepared in DMSO. A reaction mixture (200μl) was prepared with the same formula optimized for the enzyme activity assay (0.1 M Tris-HCl ph 8, 0.1 M KCI, 25 mM NaCl, 0.25 mM ATP, and two units of inorganic yeast pyrophosphatase) with 10 µM of the sample molecule. The reaction mixture was incubated for 20 minutes at ambient temperature. Enzymatic reaction was triggered by addition of the substrate B (0.2 mM) and the absorbance of the product was monitored at 290 nm for 10 minutes. Six out of 15 sample molecules showed appreciable inhibition at 10 μM (Figure 5). Three of the molecules, A3, A6, and A7 exhibited more than 50% inhibition of the enzyme activity and were further diluted to find the minimal…Results and Discussion: Folin's McCarthy Sullivan Test: Samples: proline, methionine, alanine Reagents: sodium hydroxide (5N), Glycine (1%) and 10% sodium nitroprusside solution, 6N HCl -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (5N), followed by addition of few drops of glycine (1%) and 10% sodium nitroprusside solution (HANDLE WITH CARE)and vortex. Place the test tube in a hot water bath, maintained at 40 C, for 15 minutes. Cool the test tube in ice cold water for 5 minutes and add 0.5 ml of 6N HCl. Vortex the contents and allow to stant fpr 15 minutes at room temperature.