Please describe the regulatory role of enhancers that binds to responsible regulators to activate the target gene at a given time and place through comparing different organisms according to the presence of regulatory sequences.
Q: Explain the several reasons on additional levels of complexity are both possible and necessary for…
A: Gene expression is a complex process that is mediated by several processes. There are around 20,000…
Q: A GWAS study using genomic resequencing may find a statistically significant SNP that is: (choose…
A: Introduction: A technical approach for associating specific genetic variation with a particular…
Q: The lac repressor and the pur repressor are homologous proteins with very similar three-dimensional…
A: The lac represser supermolecule, LacI, prevents the transcription of genes concerned in lactose…
Q: An electrophoretic mobility shift assay can be used to study the binding of proteins to a segment of…
A: Question - An electrophoretic mobility shift assay can be used to study the binding of proteins to a…
Q: Compare and contrast the transcriptional regulation of GAL genes in yeast with that of the lac genes…
A: The way by which a cell regulates the conversion of DNA to RNA is termed as the transcriptional…
Q: Explain why the glucocorticoid receptor binds next to the core promoter of some genes, but not next…
A: Gene expression is a process in which the information present in the particular sequences is…
Q: Both attenuation of the trp operon in E. coli and riboswitches in B. subtilis rely on changes in the…
A: The trp operon, is a group of genes found in E. coli bacteria, encoding biosynthetic enzymes for…
Q: name TWO mechanisms of control of gene expression that are only used by eukaryotes and discuss why…
A: The majority of gene expression is regulated at the transcriptional level, owing to protein binding…
Q: E27. A cloned gene fragment contains a regulatory element that is recog- nized by a regulatory…
A: Electrophoretic mobility shift assay is used to study the protein and DNA interaction i.e it detects…
Q: . Describe which enzymes are required for lactose and tryptophan metabolism in bacteria when lactose…
A: ANS 1.The enzymes of the lactose operon are needed to break down and use lactose as an energy…
Q: Explain how repressor proteins can be central to theregulation of both catabolic and anabolic…
A: Repressor proteins are those that respond to external stimuli to prevent the binding of…
Q: There are two variants of the enhancer sequence found in people. One has the sequence CACTAAAG, and…
A: Regulatory sequences or elements are the DNA sequences that serve as a binding site for regulatory…
Q: a. How many enhancers were you able to identify with these set of experiments? Explain. b. If you…
A: Enhancers are the term used for the regions of DNA (deoxyribonucleic acid) that elevate the…
Q: Enhancers are sequences that affect the initiation of the transcription of genes that are hundreds…
A: apart from the regular looping mechanism, enhancers are also capable of 3 more mechanisms that can…
Q: Explain in detail the role of super-enhancers. Give some examples. How the super-enhancers can be…
A: There are trillions of cells in the physical structure. They give the body structure, absorb…
Q: Outline the two ways that piRNAs and PIWI proteins inhibit transposable elements.
A: PIWI (P- element Induced Wimpy testis) are RNA (Ribonucleic acid) binding regulatory proteins. The…
Q: Discuss the structure and function of regulatory elements. Where are they located relative to the…
A: A eukaryotic organism is made up of several genes. It is essential to ensure that not all genes are…
Q: How do activators bind enhancers in the first place if the enhancer DNA is covered with nucleosomes?
A: In the nucleus of each cell in higher organisms, the DNA molecule is packaged into thread-like…
Q: Compare the control of gene regulation in eukaryotes and prokaryotes at the level of initiation of…
A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…
Q: he lac repressor and the pur repressor are homologous proteins with very similar three-dimensional…
A: Purine repressor (PurR) is a DNA-binding protein that is involved in the transcription process. Its…
Q: Discuss the following argument: “if the expression of every gene depends on a set of transcription…
A: The process by which a gene gets turned on in a cell to make proteins is termed as gene expression.…
Q: Upon identification of the DNA regulatory sequence responsible for translating a given gene, you…
A: The regulatory proteins binding along with enhancer sequence will result in a shift that occurs in…
Q: Explain how the expression of a single gene can be quickly,efficiently,and specifically shut down at…
A: Transcriptional in molecular biology and genetics is a process that regulates the conversion of DNA-…
Q: As discussed in the text, promoters were originally identified as consensus sequences upstream from…
A: The promoters are considered as the specific nucleotide sequence that is present in the upstream…
Q: An activator has bound to an enhancer region close to the promoter for gene A. What will this mean…
A: For the synthesis of a protein, the process of transcription and translation takes place.…
Q: The binding of a small effector molecule, protein-protein interactions, and covalent modifications…
A: Transcription factors that bind to regulatory elements are called as regulatory transcription…
Q: Describe with your own words two ways to silence the expression of gene X through regulatory RNA…
A: Gene expression is a process in which gene is transcribed into RNA and RNA is translated into…
Q: An enhancer is surrounded by four genes (A, B, C, and D), as shown in the accompanying diagram. An…
A: Transcription is promoted by the short sequences of gene in the DNA. The transcriptional activator…
Q: One of the main lessons of this chapter is that several bacterial genes are often transcribed from a…
A: As given: Several bacterial genes are often transcribed from a single promoter into a large…
Q: You have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu…
A: In this question, we are given with a situation in which the DNA has two mutation reg1 and reg2.…
Q: List two different ways in which trans-acting small RNAs(sRNAs) regulate the expression of target…
A: Trans-acting small RNAs or ta-siRNA are a class of siRNA that is responsible for repressing gene…
Q: Match the term with its description. cAMP Allows lactose to enter the cell and product of the…
A: Cyclic Adenosine monophosphate is a second messenger which is necessary for various biological…
Q: which laboratory method can be used to quantify levels of mRNAs expressed in samples of two…
A: Biological approaches are a well-developed field in process technology for treating a wide range of…
Q: Can methylation state of the promoter influence the level of gene expression? Briefly describe how…
A: In eukaryotes, DNA methylation occurs by an enzyme known as DNA methyltransferase. Where the…
Q: Describe the methods used to identify the location of DNA binding proteins in the regulatory regions…
A: DNA binding sites are a type of binding location found in DNA where other molecules may also bind.…
Q: Describe the methods used to identify the location of transcription-control elements in…
A: Transcriptional control elements are the sequence of nucleotides in a gene that is involved in the…
Q: Negative supercoiling of DNA favors the transcription of genes because it facilitates unwinding.…
A: Transcription can be defined as the process in which RNA is formed from the DNA. This is the first…
Q: Describe in detail the role of super-enhancers. Give some examples. How the super-enhancers can be…
A: Super-enhancers (SEs) in a wide scope of human cell types are enormous bunches of enhancers with…
Q: Match whether the protein binds directly or indirectly (assuming it does) to DNA and whether to the…
A: Enhancers are commonly known as cis-acting DNA sequences that can boost gene transcription. To begin…
Q: The UG4 gene is expressed in the stem and leaf tissue of the plant Arabidopsis thaliana. To identify…
A: A mutation is a change in the sequence of a living organism's genetic material caused by an…
Q: What role does an operator sequence serve in bacterial gene expression regulation? Describe one…
A: As per the honour code, we are entitled to do only one question at a time. So, I am providing the…
Q: Compare and contrast the role of promoter and operators with enhancers and repressors.
A: Nucleus is a main controller of the cell which carries genetic instructions . It contain thread like…
Q: you design an ideal promoter for a gene to be highly expressed in E. coli cells? Describe strategies…
A: Which Organism to Use? The preference of the host molecular whose protein synthesis equipment will…
Q: Which mechanisms for regulating gene expression may be applied for the treatment of such diseases?…
A: Gene expression is the process by which information from a gene is used to make a protein. Gene…
Q: What is the role of a promoter (regulatory region) of a gene?
A: The fundamental unit of heredity is Gene. It is transferred from the parents to the offspring and is…
Q: What is an enhancer? What do these sequences contain bound to it? Do enhancers have to be near…
A: In the human genome, there are around 110,000 gene enhancer sequences. They are regulated by…
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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- In the galactose operon of E. coli, a repressor, encoded by the galR gene, binds to an operator site,galo, to regulate the expression of three structuralgenes, galE, galT, and galK. Expression is inducedby the presence of galactose in the media. For eachof the strains listed, would the cell show constitutive, inducible, or no expression of each of the structural genes? (Assume that galR− is a loss-of-functionmutation.)a. galR−galo+galE+galT+galK+b. galR+galocgalE+galT+galK+ c. galR−galo+galE+galT+galK−/galR+galo+galE−galT+galK+d. galR−galocgalE+galT+galK−/galR+galo+galE−galT+galK+In addition to Tc1, the C. elegans genome contains otherfamilies of DNA transposons such as Tc2, Tc3, Tc4, andTc5. Like Tc1, their transposition is repressed in thegerm line but not in somatic cells. Predict the behaviorof these elements in the mutant strains where Tc1 is nolonger repressed due to mutations in the RNAi pathway.Justify your answer.Based on its sequence similarity to Apobec1,which deaminates Cs to Us in RNA, activation-induceddeaminase (AID) was originally proposed to work on RNA.But definitive experiments in E. coli demonstrated thatAID deaminates Cs to Us in DNA. The authors of the paperexpressed AID in bacteria and followed mutations in aselectable gene. They found that AID expression increasedmutations about fivefold above the background level inthe absence of AID expression. More importantly, theyfound that 80% of the induced mutations were G→A orC→T. Does this fit with your expectation if AID-inducedmutations arose by deamination of C to U in the DNA?
- Each year in the United States, there are over 230,000 newcases of prostate cancer and almost 28,000 deaths. A 3.8-Mbregion on chromosome 8 (8q24), called a gene desert, has genes but contains enhancer sequences that potentiallyconfer significant risks for prostate cancer. One particular enhancerallele, which is known to be associated with an elevated risk forprostate cancer, physically interacts with the promoter region ofthe nearby MYC gene and facilitates its upregulation. Overexpressionof MYC, which encodes a cell-cycle regulatory protein, isobserved in multiple types of cancer (see Chapter 24). The riskallele has a frequency of 49 percent in men of European descentand 81 percent in men of African ancestry. Most of the differentialMYC activity associated with the risk allele occurs during prenataldevelopment, raising the possibility that testing for this alleleearly in life can be used to identify those in the African-Americanpopulation who are at very high risk for prostate…One of the main lessons of this chapter is that severalbacterial genes are often transcribed from a singlepromoter into a large multigene (polycistronic) transcript. The region of DNA containing the set of genesthat are cotranscribed, along with all of the regulatoryelements that control the expression of these genes, iscalled an operon.a. Which of the mechanisms in the following listcould explain differences in the levels of themRNAs for different operons?b. Which of the mechanisms in the following listcould explain differences in the levels of theprotein products of different genes in the sameoperon?i. Different promoters might have different DNAsequences.ii. Different promoters might be recognized bydifferent types of RNA polymerase.iii. The secondary structures of mRNAs might differ so as to influence the rate at which they aredegraded by ribonucleases.PROBLEMSProblems 575a. Why did the mating with a nonlysogenic recipientresult in phage growth and release, but the infectionof a…Many viruses that infect eukaryotic cells express genes that alterthe regulation of host gene expression to promote viral replication.For example, herpes simplex virus-1 (HSV-1) expresses aprotein called ICP0, which is necessary for successful viral infectionand replication within the host. Lutz et al. (2017. Viruses9: 210) showed that ICP0 can act as a ubiquitin ligase and targetthe redundant transcriptional repressors ZEB1 and ZEB2,which leads to upregulation of the miR-183 cluster (a set of threemiRNAs transcribed from the same locus). Speculate on how miR-183 cluster upregulation may benefitthe virus.
- The TBX20 transcription factor is important for the developmentof heart tissue. Deletion of the Tbx20 gene in mice results in poorheart development and the death of mice well before birth. Tobetter understand how TBX20 regulates heart development ata genetic level, Sakabe et al. (2012. Hum. Mol. Genet. 21:2194–2204) performed a transcriptome analysis in which they comparedthe levels of all mRNAs between heart cells from wild-typemice and mice with Tbx20 deleted. This study concluded that TBX20 acts as an activator ofsome genes but a repressor of other genes in cardiac tissue.How might a single transcription factor have oppositeeffects on the transcription of different genes?A cloned gene fragment contains a regulatory element that isrecognized by a regulatory transcription factor. Previousexperiments have shown that the presence of a hormone resultsin transcriptional activation by this transcription factor. To studythis effect, you conduct an electrophoretic mobility shift assayand obtain the following results: Explain the action of the hormone.ne of the main lessons of this chapter is that several bacterial genes are often transcribed from a single promoter into a large multigene (polycistronic) transcript. The region of DNA containing the set of genes that are cotranscribed, along with all of the regulatory elements that control the expression of these genes, is called an operon. a. Which of the mechanisms in the following list (i-vi) could explain differences in the levels of the protein products of different genes in the same operon? Different promoters might have different DNA sequences Different promoters might be recognized by different types of RNA polymerase The secondary structures of mRNAs might differ which will influence the rate at which they are degraded by ribonucleases. In an operon, some genes are farther away from the promoter than other genes The translational initiation sequences at the beginning of different open reading frames in an operon might result in different efficiencies of translation Proteins…
- The following statement occurs early in this chapter:“. . . a crucial step in the regulation of many bacterialgenes is the binding of RNA polymerase to DNA atthe promoter.” Why might it be advantageous forbacteria to regulate the expression of their genesat this particular step?. In an effort to determine the location of an operator sitefor a negatively regulated gene, you have made a seriesof deletions within the regulatory region. The extent ofeach deletion is shown by the line underneath the sequence, and the resulting expression from the operon(i = inducible; c = constitutive; − = no expression) isalso indicated.... GGAT C T T AGCCGGCTAACATGATAAATATAA......C C T AGAATCGGCCGA TTGTA C T A TTT ATAT T ...1 i2 –3 c4 –5 ca. What can you conclude from these data about thelocation of the operator site?b. Why do you think deletions 2 and 4 show no expression of the gene?iestiðn Pre-initiation complex (PIC) is formed right before the initiation of genes, which of the following steps seemes to NOT help for the PIC formation? O TBP protein to recognize TATA box at promoter region O TFIIA to recognize the TAFS in TFIID O Enhancers to attract coactivators and activators O Silencers to attract repressor and corepressors