One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N culture and then resuspend them in buffer P1. What's the point of pelleting the cells just to resuspend them again? To concentrate the cells O P1 is a buffer, preventing small changes in pH O This step is not absolutely necessary P1 begins the lysis process Question 2 What would happen if we didn't centrifuge the tube containing lysed cells after adding neutralization buffer and instead just added right to the column? O We could fihish the prep but we would have protein/RNA contaminants at the end O The precipitate is less dense than water so it shouldn't get in the way since it floats on top O The precipitate would obstruct the column and our prep is ruined O Our yield would be reduced, but we'd get something

One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N culture and then resuspend them in buffer P1. What's the point of pelleting the cells just to resuspend them again? To concentrate the cells O P1 is a buffer, preventing small changes in pH O This step is not absolutely necessary P1 begins the lysis process Question 2 What would happen if we didn't centrifuge the tube containing lysed cells after adding neutralization buffer and instead just added right to the column? O We could fihish the prep but we would have protein/RNA contaminants at the end O The precipitate is less dense than water so it shouldn't get in the way since it floats on top O The precipitate would obstruct the column and our prep is ruined O Our yield would be reduced, but we'd get something

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter20: Viruses, Bacteria, And Archaea

Section: Chapter Questions

Problem 6SQ

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explain F' plasmid transfer diagramatically
16. Bacterial cell wall membrane includes all EXCEPT ( Mark both answers ) Group of answer choices O- polysaccharides porins lipoproteins asymmetric phospholipids membrane lipid A teichoic acids 18. F plasmid is best desribed as a ……………... plasmid Group of answer choices non self transmissible self transmissible virulence plasmid becos it codes for toxin promiscuous high copy number 19. Identify the FALSE statement from the following, Group of answer choices self transmissible plasmids have origin of transfer as well as mobilisation genes inclusions are non functional, storage granules accumulated inside bacterial cells plasmid DNA are in nucleoid area in Bacillus anthracis R plasmid codes for the sex pili host range refers to ability of plasmid to multiply in cell types 21. Which one of the following statement regarding plasmid is FALSE, Group of answer choices they are small, extrachromosomal DNA molecules they are necessary for…
Activity 2 Study the following diagram which outlines gene cloning then, answer the questions that follow: DNA fragments from so urce DNA DNA inser ted into plasmid vector SES Transformation D Colle cfror DNA Fragmen Each cell contains a single fragment. All cells together are the librar y. 1. Follow the steps involved in DNA or gene cloning. 2. In what process do bacteria take up the recombinant plasmid DNA? 3. What are the advantages or applications of gene cloning?
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Activity 2 Stady the following diagram which outlines gene cloning then, answer the questions that follow: DNA fragments from so urce DNA DNA inser ted into plasmid vector Transformation D Colle cfror DMA Fragmen Each cell contains a single fragment. All cells together are the librar y. sbold 1. Follow the steps involved in DNA or gene cloning. 2. In what process do bacteria take up the recombinant plasmid DNA? 3. What are the advantages or applications of gene cloning?
explain F' plasmid formation
Besides heat shock method, elaborate another procedure commonly used for transformation vector a host cell. of a plasmid into
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