[O2CC(O)CH2CO2] 2− + H2O → C2O44−+ CH3COO− + H+ oxaloacetate oxalic acid acetate In a 1-ml reaction mixture containing 50 mM NaCl, 50 mM HEPES, pH 7.8, 5 mM oxaloacetate and 5 pmol oxaloacetase, complete hydrolysis of the oxaloacetate was achieved in 10 minutes of incubation at 30°C. What was the pH of the reaction mixture at the end of the reaction?
HEPES, 4-(2-hydro)-1-piperazine ethane sulfonic acid, shown below. fully protonated, has pKa’s of 3.00 and 7.55. It is a commonly used buffer for tissue culture and organ storage during transplantation due in part to its effectiveness over a wide temperature range. One-way oxalic acid can be produced in fungi by hydrolysis of oxaloacetate, an intermediate in the Kreb’s citric acid cycle, to produce oxalic acid and acetic acid. This reaction, shown below, is catalyzed by oxaloacetase.
[O2CC(O)CH2CO2] 2− + H2O → C2O44−+ CH3COO− + H+
oxaloacetate oxalic acid acetate
In a 1-ml reaction mixture containing 50 mM NaCl, 50 mM HEPES, pH 7.8, 5 mM oxaloacetate and 5 pmol oxaloacetase, complete hydrolysis of the oxaloacetate was achieved in 10 minutes of incubation at 30°C.
What was the pH of the reaction mixture at the end of the reaction?
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