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- 1.Which of the following correlations about the analyses of iodine and saponificationnumbers is/are incorrect?a. Alcoholic KOH: dissolve KOH to lipid sampleb. Hanus reagent: react with saturated lipid bondc. Reflux set-up: hasten reactiond. Blank titration: determine total mol of KOH2. A triacylglycerol (Sample X) was subjected to saponification analysis. 1.1 grams of thesample was refluxed with 5% alcoholic KOH and the refluxed solution was titrated with 17.2 mLof 0.44 M HCl titrant. The blank solution was titrated with 25.6 mL of the titrant.i. What is the saponification number of the sample? ii. Which of the following could be the identity of the sample? Show solutions pls a. Tripalmitin (MW: 807.3)b. Trilaurin (MW: 639.8)c. Triolein (MW: 885:4)d. Tristearin (MW: 891.48)(11) Find Unknovong P Note: PfX Bothgive Posidive Test with ammonical AgNDa Solution. Hg S04The following data give the recovery of bromide from spiked samples of vegetable matter, measuredusing a gas–liquid chromatographic method. The same amount of bromide was added to each specimen(Roughan, J.A., Roughan, P.A. and Wilkins, J.P.G., 1983, Analyst, 108: 742).Tomato: 777 790 759 790 770 758 764 ug/gCucumber: 782 773 778 765 789 797 782 ug/g(a) Test whether the recoveries from the two vegetables have variances which differ significantly.(b) Test whether the mean recovery rates differ significantly.
- Chromatography question A student properly sets up a column chromatography experiment. Although the mixture is soluble i the mobile phase, the sample appears "stuck" at the top of the column and will not elute eregardless of how much solvet is pplaied. what could bee the cause of this issue and what could teh student try to achieve a successful separation?1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?
- 1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →Identify these statements with true or false true or false I. Chromatography utilizes partition coefficient theory true or false II. In reverse phase chromatography, the mobile phase is a polar solvent true or false I. Aspirin is an ester prodrug. true or false Aspirin is synthesized by means of an acid catalyzed reaction. true or false I. Grain alcohol boils at 78.3 C true or false Wood alcohol boils at 60 C true or false Diazo coupling involves a sigma and a pi bond in two nitrogen atoms true or false When methyl orange passes through from basic to acidic medium, the change in color is from yellow to intense red true or false Benzoic acid is a white crystalline substance that has a melting point of 122 C true or false Benzoic acid can be prepared by the alkaline reduction of toluene true or false Potassium permanganate is an oxidizing agent true or false Potassium permanganate should never be mixed with glycerin true or false Methyl…
- 2. What are mobile and Stationary phases in a chromatogram? Site their nature.which of the following froactions is equivalent to 55% 13/25, 11/20/, 9/20, 12/25How else can chromatography be used? None of these answers are correct. To wash (or remove) unwanted solvents from a mixture. To identify or exclude components in a mixture. To generate IUPAC names for hydrocarbons.