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What is the importance of pH adjustment in culture media?
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- A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5% agar, and distilled water. If you are asked to prepare 765 mL of culture media, how much meat extract would you need?What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?If cultures are suffering severely due to alkaloid production and accumulation in the medium then, how can i overcome this problem?
- What are the advantages and disadvantages of the filter method in the analysis of water samples? Why is the incubation temperature critical for this test?What advantages do solid media offer for the isolation of microorganisms? How will you check the purity of an isolate?What are the immersion media that need to be used?