Mix 0.5 ml of cold saturated sulfanilic acid solution (HANDLE WITH CARE) with 0.25 ml of cold 1.0% NaNO2. Cool in ice with constant shaking for 3 minutes. Add 0.5 ml of the sample and make alkaline with 10% Na2CO3. Record your observation.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Result and Discussion:
Pauly Reaction:
Samples: tyrosine, alanine, histidine
Reagents: Cold Saturated Sulfanilic Acid, Cold 1 % Sodium Nitrite (NaNO2) and 10% Sodium Bicarbonate (Na2CO3)
- Mix 0.5 ml of cold saturated sulfanilic acid solution (HANDLE WITH CARE) with 0.25 ml of cold 1.0% NaNO2. Cool in ice with constant shaking for 3 minutes. Add 0.5 ml of the sample and make alkaline with 10% Na2CO3. Record your observation.
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