Metabolic engineering Consider the engineering design of microbial cell culture and growth in the field of biotech- nology and metabolic engineering. Bioreactors are employed incorporating the use of bio- chemical substrates that provide essential chemical elements for the growth of the microor- ganisms. A typical stoichiometric representation of the aerobic growth of a microorganism with a defined chemical formula is shown below С, Н, ОН + а0, b NH, → cCH, No.15O04 + dH,0 + eCO, (C-source) (O-source) (N-source) (Microorganism) The ratio of moles of CO2 produced per mole of O2 consumed is called the respiratory quotient, RQ, which can be experimentally determined, and is therefore treated as a known quantity. To determine the theoretical yield of production (growth) of the microorganism, the stoichiometric coefficients, a, b, c, d are required. One can easily formulate an elemental mass balance for each of the four key elements. (a) Formulate an elemental mass balance for each of the four key elements. (b) Solve the system for RQ = 2.7.
Metabolic engineering Consider the engineering design of microbial cell culture and growth in the field of biotech- nology and metabolic engineering. Bioreactors are employed incorporating the use of bio- chemical substrates that provide essential chemical elements for the growth of the microor- ganisms. A typical stoichiometric representation of the aerobic growth of a microorganism with a defined chemical formula is shown below С, Н, ОН + а0, b NH, → cCH, No.15O04 + dH,0 + eCO, (C-source) (O-source) (N-source) (Microorganism) The ratio of moles of CO2 produced per mole of O2 consumed is called the respiratory quotient, RQ, which can be experimentally determined, and is therefore treated as a known quantity. To determine the theoretical yield of production (growth) of the microorganism, the stoichiometric coefficients, a, b, c, d are required. One can easily formulate an elemental mass balance for each of the four key elements. (a) Formulate an elemental mass balance for each of the four key elements. (b) Solve the system for RQ = 2.7.
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter4: Energy And Chemical Reactions
Section: Chapter Questions
Problem 120QRT
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