Match the part of the microscope with its use. Dragged and dropped options on the right-hand side will be automatically saved. For keyboard navigation... SHOW MORE Stage Nosepiece Vertical Stage Knobs Course and Fine Adjustment Knobs Iris Diaphram III III ||| Allows for changing objective lenses Move the stage side to side Hold the slide in place Control light passing through the stage Move the stage up and down
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- Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.Using the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use. Record your observations and notes as vou will require this information when writing yourlab reportComparison of the different objectives of the compound microscope Point of Comparison Scanner LPO НРО O1O Degree of magnification (X) 4х 10x 40x 100x Details of the specimen (least to most detailed) Area of field of view (smallest to biggest) Brightness of image (dimmest to brightest ) Working distance (mm)
- Which of these is NOT a best practice when using, handling, or storing a microscope and microscope slides? Do not touch the objective lenses. Store the slides in bright sunlight to prevent mold growth. Rotate the nosepiece to the lowest objective before storing the microscope. Keep dust away from the scope by covering it during storage.Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical apertureThe compound (bright-field) microscope is parfocal. It means that: Question 4 options: objective lenses are built into revolving nosepiece its lenses effectively decrease focal lenght can achieve maximum resolution lower than 0.2 μm image should remain in focus when objective lens is changed
- An object found with the 10x objective of the microscope may be viewed with the 40x objective without having to re-focus. This property of the microscope is called: O Confocal O Bifocal Binocular d ParfocalWhen carrying a microscope you should always have one hand on the base and the other on the arm. True FalseWhat is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-Probe
- A student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometers3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?