John was repeating George Palade's pulse and chase experiment to trace insulin secretion pathway within cells. When he observed his samples under the electron microscope, he saw the³H]-leucine labelling all over the cells, which indicates that he made some mistakes in carrying out the experiment. Which of the following is a correct suggestion to improve his result? O Use liver sample after the pulse-chase injection O Inject [13C]-leucine instead of [3H]-leucine O Inject non-labeled leucine earlier than what he did Use phase contrast light microscope instead of electron microscope Observe the sample two days after pulse-chase injection
John was repeating George Palade's pulse and chase experiment to trace insulin secretion pathway within cells. When he observed his samples under the electron microscope, he saw the³H]-leucine labelling all over the cells, which indicates that he made some mistakes in carrying out the experiment. Which of the following is a correct suggestion to improve his result? O Use liver sample after the pulse-chase injection O Inject [13C]-leucine instead of [3H]-leucine O Inject non-labeled leucine earlier than what he did Use phase contrast light microscope instead of electron microscope Observe the sample two days after pulse-chase injection
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.4P
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The pulse-chase experiment is a technique used to monitor the progress of newly made proteins within cells. It consists of a short period of labeling the substances used in protein production, followed by a chase with unlabelled substances. This experiment allows us to examine the synthesis, stability, movement, and the breakdown of proteins within cells.
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