IR Sepectrometer organic

Q: What is the mobile phase for the separation shown and the relative retention between peak 3 &4?

A: Generally mobile phase is an organic solvent here, so many solvents are used for the mobile…

Q: What cause the spikes in the spectrophotometer reading? Explain

A: In electronic spectroscopy, generally the electron excites  from an initial low energy state to a…

Q: 42 Pdlc lo%. Erou,u ce

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Q: show the instrumentation of HPLC providing a schematical representation ?

A: Instrumentation:-

Q: An analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The…

A: TLC is thin layer chromatography technique which is used to separate the mixture of non-volatile…

Q: Which of the following methods cannot be used to visualize spots on a TLC plate? Group of answer…

A: Which of the following methods cannot be used to visualize spots on a TLC plate?   Group of answer…

Q: What is the Rf value for the uppermost yellow spot in TLC plate 3 (plate imaged below)? It is…

A: The TLC plate 3 with all the spots is given below :

Q: 443 471 502 360 Wavelength (nm) Lycopene Amax is 502 nm True False Absorbance

A: UV visible specctroscopy is used for quantitative determination of different samples.It is also…

Q: Show TWO (2) characteristics of the core enhanced technology that contribute to better separations,…

A: A question based on analytical separations that is to be accomplished.

Q: PURITY TEST Instructions: Note the REFRACTIVE INDECES of each of the samples and solve for their %…

A:

Q: Is the separation of the mixture shown here a physical orchemical process?

A: SOLUTION: Step 1: The separation of the mixture shown above (i.e. by column chromatography) is a…

Q: analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The…

A:

Q: Which is the more reliable technique for identifying an unknown compound and why? IR spectrum…

A: Every technique is important to identify a new compound.  But the answer of the question is IR…

Q: 12. This detector is not used in HPLC: a) ECD b) MS c) Fluorescence d) RI

A: Mixture components are estimated using HPLC detectors. The actual separation in HPLC (High…

Q: Solvent Front Highest point of the dye Lowest point of the dye Rf Value Baseline Chromatogram A 15.0…

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Q: Table. Absorbance data for iron standards and flour samples Fe Conc. (mg/L) Corrected Sample…

A: Absorbance of a substance is proportional to its concentration and path length (width of cuvette) by…

Q: fill in the column thats empty by finding the standard deviation for each average absorbance, with…

A: Concentration of Dye  Average Absorbance of replicate   Standard division 1mg/L 0.269 0.731…

Q: DH trotch| a 3325 tetch R Spectrum (liquid film) 0.아 2000 1600 V (cm") 000 3000 1200 800 laromatic…

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Q: The widths at half-height, wi/2, of the peaks for compounds A and B are 0.123 min and 0.299 min,…

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Q: 4000 100 80 IR Spectrum (Iquid film) 8888 % of base peak 20 40 3000 80 2000 105 120 m 1600 1200 V…

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Q: It is acceptable to draw the TLC spotting line with a pen as long as it is marked very lightly.…

A: False

Q: Calculate the Rf value of a spot if it travelled 1.5 cm while the solvent travelled 2.5 cm. Express…

A: Given :  1. Spot distance = 1.5 cm  2. Solvent distance = 2.5 cm

Q: 10 9. 8 7 6. 4 3. 1 0 ppm Chemical shift (8)

A: The given information is: Chemical formula of the compound = C8H9Br 4 chemical shifts are present:…

Q: What types of flow meters are used in gas chromatography?

A: ANSWER IS

Q: The standard graph contains absorbance values greater than 1.00 Is there a positive error, negative…

A: Yes, absorbance greater than 1 is possible, it's not an error. It just means your solution is too…

Q: It refers to the general term of adsorbent media used to visualize chromogenic compounds. A.…

A: Chromatogram refers to the general term of adsorbent media used to visualize chromogenic compounds.…

Q: 1. Provide two advantages and two disadvantage of flow cytometry analysis. Briefly explain each.

A:

Q: Preparing a Thin Layer Chromatography experiment there are some common mistakes. Explain what…

A: Interpretation- To tell about all the common mistakes when we prepare a thin layer chromatography…

Q: Estimate a diameter of a 2 denier circular crossection fiber made of Nylon 6 (density = 1.14 g/cm3).…

A:

Q: It is used with samples in MALDI system to help ionization of the sample and then irradiated with…

A: The objective of the question is to select one of the given options based on the given statement…

Q: Column A Cesponse Unretained peaks Compound Y Compound X Time (min) 8 min. Compound Y Column B…

A: We can decide the effectiveness of resolution in column chromatography based on the nature of the…

Q: Why are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table…

A: The function of detectors used in high-performance liquid chromatography is to monitor the mobile…

Q: PIGMENT SPOT PHI   WORK   DUEAN   MEEN

A: The crude reaction mixture has two or more reactions combined in a different molar ratio. Generally,…

Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.18 sec? The…

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Q: At the completion of the experiment, a student measures the solvent front to be 55 mm from the…

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Q: IR Spectrum (KBr disc) 4000 3000 2000 Amin amm 1200 800 1722 1680 1600 V (cm¹)

A: A question based on IR spectroscopy that is to be accomplished.

Q: IR spectra (KBr disc) LOD D 4800 3000 2000 1000 500 MAVENUNB ERI l

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Q: Mode of separation that uses a polar stationary phase: IEX HILIC SEC NPLC

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Q: A technician injects a sample twice back to back into a C18 General Purpose HP column. Sample…

A: One extra peak in first injection may be due to the presence of contamination from septum, injection…

Q: What is the Rf of the compound if the compound distance is 2.59 cm and the solvent front distance is…

A: Given that, Distance travelled by solute= 2.59 cm Distance travelled by solvent= 5.81 cm Then ,…

Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.73 sec? The…

A: Answer: Retention factor is equal to the ratio of difference (between retention time and void time)…

Q: A brief introduction on high performance liquid chromatography ( HPLC)

A: It is as follows

Q: Does the amount of radiation (cpm) increase or decrease as the distance between the sorce and the…

A: Amount of radiation between the source and sensor is determine using the inverse square formula:…

Q: Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm.

A: Rf is a retardation factor that is defined as the distance traveled by a substance divided by the…

Q: How column is developed and solvent selection is done in HPLC?

A: HPLC stands for High-pressure liquid chromatography or high-performance liquid chromatography.HPLC…

Q: Rf of spot A in the TLC plate below is: Solvent front A 10 cm OB 8 cm 6 cm 2 cm Origin Select one: O…

A: The Rf value of a component depends on the distance travelled by the component and the distance…

Q: analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU)…

A: concentration of mixture= C1V1+C2V2+C3V3+C4V4/(V1+V2+V3+V4)

Q: Draw a skitch for GC and HPLC instruments showing all parts

A: GC and HPLC instrumentation can be given as follows

Q: Data Sheet: Assuming that the following data were the result of the experiment, compute for the %…

A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: How do you weigh hydgroscopic samples?

A: Hydgroscopic samples contain moisture. Therefore it is measured in digital analytical balance.