In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.
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In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.
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- The validation process comprises four main steps that are : hardware validation, software validation, method validation and system suitability. To evaluate a spectrophotometer’s accuracy by measuring absorbance, a solution of 60.06 mg/L K2Cr2O7 in 0.0050M H2SO4 was carefully prepared together with a 0.0050 M H2SO4 as a reagent blank. If the spectrometer’s signal is properly calibrated, it has been reported that this solution should provide an absorbance of 0.640 +- 0.010 at wavelength 350 nm. Which one of the validations mentioned above had been involved in this process? Explain your choiceIn the process of performing a spectrophotometric determination of Fe, an analyst prepares a calibration curve using a single-beam spectrometer, such as a Spec-20. After preparing the calibration curve, the analyst drops the cuvette used for the method blank and the standards. The analyst acquires a new cuvette, measures the absorbance of the sample, and determines the %w/w Fe in the sample. Will the change in cuvette lead to a determinate error in the analysis? Explain.What are the 5 important characteristics of spectrophotometric methods
- 3. The lead in a swab sample, lead standards, together with a blank were made up in 5.00 mL volumetric flasks using 0.2 % HNO3. 20 μL aliquots of these solutions were injected into the spectrophotometer and the absorbance measured at 217 nm. The following results were obtained. lead / ppb Absorbance 0 0.0591 10.00 0.0858 50.00 0.1926 100.0 0.3260 150.0 0.4594 200.0 0.5929 Swab sample 0.3700 (a) Determine the amount of lead in the swab sample in μg.What are the main components of a spectrophotometer? Choose all that applies. radiation source sample compartment/holder monochromator detector0.1120 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL volumetric flask and volume was made up with distilled water. The solution was then diluted by a factor of 25. The final solution was analyzed by atomic absorption spectrophotometry and the concentration was found be 2.982 ppm, What is the weight percentage of copper in the original solid sample? Keep four significant figures in your final answer.
- One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all of the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectivelywhy does the choice and use of a blank matter for Spectrophotometry?In order to measure riboflavin in a breakfast cereal, a 5.00-gram sample of the cereal was extracted with acetic acid and filtered. The extract was transferred to a 50.0 mL volumetric flask and diluted to the mark. The sample was transferred to a cuvette and the fluorescence was measured. The instrument read 59 units (Fluorescence units –an arbitrary scale). 24.00 mL of the cereal extract was sample was mixed with 1.0 mL of a riboflavin standard that was 0.500 ug/mL. The fluorescence of this mixture was measured and read 94 fluorescence units. Calculate the concentration of riboflavin per gram of cereal (use µg/gram of cereal).
- What types of samples can be analyzed by IR?How are substances that are opaque (nottransparent) handled using this technique?One method for the quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is area normalization. Here, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction involves dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2 and 30.2, in order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78 and 0.88, respectively.Suppose a student is preparing a set of standard solutions for use in a spectrophotometric study. If percent transmittance, %T, were measured in the standard solutions before the color had a chance to fully develop, the measured value would be compared with solutions that have allowed the color to fully develop.
