If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?
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If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?
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- You are asked to calibrate the ocular micrometer for the 4x objective using the stage micrometer. You observe 32 stage micrometer units line up with 15 ocular micrometer units. What is the value for each division of the ocular micrometer for this objective? (Round to the 1 decimal place)The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?How many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?
- What are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?How can you tell if the current or the gel electrophoresis is already running and what are the common mistakes or problems encountered in gel electrophoresis analysis?compute for the callibration factor in micrometers of the ocular micrometer using the low power objective. explain your answer
- The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is 2m long. What is the magnification factor of the cell on the screen? Practice O 1 000x O 4 000x O 10 000x O 40 000x 100 000x O400 000xIf you observe a specimen under this 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?Why is the accuracy of the calibration curve so critical?
- You look through a compound microscope and count 8 cells that fit across the diameter of your field of view with the 40X lens. Your field diameter is a 4X lens and is 5 mm. Answer the following questions below: (show work for full credit): What is the field diameter at 40X? What units are you using? mm or um? ( make sure to explain your answer) What is the formula for calculating cell size?What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.With the constants you have for each objective, compute for the sizes of the given cells below. (Refer to the illustrations & show your solutions). Also, name each specimen used.