How is the Bicinchoninic acid assay used in protein quantification?
Q: What is molecular pharming? Compared with the production of proteins by bacteria, why might it be…
A: The production of foreign proteins by bacteria involves selection of the desired gene, incorporation…
Q: How can the binding assay approach be utilized to match coding triplets with their amino acid…
A: Live cells use the genetic code to turn information encoded in genetic material (DNA or mRNA codon…
Q: What is the point of hydrolyzing RNA before doing biochemical tests?
A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.
Q: How are dideoxynucleotide triphosphates utilized in sequencing?
A: DNA is the genetic substance of all creatures. It is a double-helical structure made up of two…
Q: In assessing extracted DNA quality, what does A280 indicate? aromatic amino acids nitrogenous bases…
A: DNA is extracted from specific cells to study the extracted DNA. The purity of extracted DNA is…
Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?
A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…
Q: What are recombinant proteins? How do bioreactors help in their production?
A: The study that involves the molecular level activities that occur in living beings is called…
Q: How do you verify amino acid sequences with proteins and species with BLAST?
A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…
Q: What are gene microarrays? How are microarrays used?
A: The process of forming a single double-stranded nucleic acid molecule by combining two…
Q: What are ‘Selectable marker’? What is their use in genetic engineering?
A: Genetic engineering is a technique to manipulate the genetic content of an organism resulting in…
Q: How does high throughput screening work?
A: High-throughput screening (HTS) is a automated process to test large numbers compounds (chemical or…
Q: Which enzyme is responsible for Supercoiling?
A: DNA supercoiling is over or under coiling of the DNA strand to produce a negatively supercoiled…
Q: What is the relevance of the sample size to the choice of test organisms for genetic studies?
A: The sample size is the number of organisms chosen for a study or experiment. It can be large or…
Q: Describe the two general types of protein microarrays. What aretheir possible applications?
A: A protein microarray is also known as the protein chip, is the high throughput method that is used…
Q: What is meant by the term genetic testing? How do testing at theprotein level and testing at the DNA…
A: Medical genetics is one of the branches of medicine, which involves the management and diagnosis of…
Q: Why are temperature-sensitive mutations useful for uncovering thefunction of a gene?
A: Mutations are alterations in the DNA sequence due to the mistakes during cell division, exposure to…
Q: What is oligonucleotide microarray?
A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…
Q: Why are the proteins cleaved into small fragments for protein sequencing?
A: Proteins are the end product of the molecular translation that codes the information present in the…
Q: How can the foreign protein be modified to facilitate its purification?
A: Protein purification is a progression of cycles proposed to confine one or a couple of proteins from…
Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?
A: Many assisted reproductive techniques are widely used around the globe.
Q: What is pharmacogenomics and how might it benefit patient care?
A: Pharmacogenomics is the study of how genes affect a person's response to drugs. This relatively new…
Q: How are molecular probes prepared?
A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…
Q: Why is DNA microarray an important tool and explain How is DNA microarray used in detecting cancer?
A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…
Q: How can the binding assay technique be used to assign coding triplets to the corresponding amino…
A: Different triplet corresponds to different amino acids. In this method, an amino acid that is…
Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?
A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…
Q: How would you simply explain Protein Analysis (Western blotting)?
A: Proteins, also known as polypeptides, are made up of amino acids. They’re large, complex molecules…
Q: Explain the Triplet Binding Assay ?
A: Introduction In 1964, two eminent scientists Marshall W. Nirenberg and Philip Leder carried out the…
Q: What class of genes is used in MLST analyses?
A: MLST refers to multilocus sequence typing. MLST involves higher frequency of recombination in…
Q: Why is it necessary to examine gene-expression profiles, in additionto genome sequences, for…
A: Gene expression profiling is the analysis of the behaviour of thousands of genes at once to…
Q: What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of…
A: Step 1 DNA or deoxyribonucleic acid occurs in living systems and can function as genetic materials.…
Q: What is in situ hybridization used for and how does In Situ Hybridization detect?
A: Hybridization, in general, is a process during which a moiety is formed having combined traits of…
Q: How proteins are analyzed using gel electrophoretic techniques?
A: Electrophoresis is a method of separating solutes based on their charge by applying an electric…
Q: How does the shotgun approach differ from the whole genome sequencing?
A: Biotechnology is concerned with exploitation of biological agents or their components for generating…
Q: What is the purpose of hydrolyzing the RNA before conducting biochemical tests?
A: Introduction: RNA is a type of nucleic acids that is a genetic material in some viruses.
Q: how many methods for detecting point mutations?
A: A single base pair is changed in a point mutation. Point mutations can also have three different…
Q: What are the main differences between bottom up/shotgun and top down proteomics strategies?
A: Proteomics studies plays an important role in biomarkers and drug targets. Mass spectrometry (MS) is…
Q: What is a microarray? How can it be used to obtain information about gene function?
A: A gene is a specific nucleotide sequence in RNA or DNA. It is generally located on a chromosome.…
Q: What is Metabolomics ?
A: Introduction: Humans are multicellular organisms with very large bodies that need a lot of oxygen to…
Q: What are Short hybridization probes?
A: A probe is a stretch of single stranded ribonucleotides (RNA) or deoxyribonucleotides (DNA). DNA…
Q: What is the Ames test and how is it carried out? What assumption concerning mutagenicity and…
A: Mutagens are chemical compounds that cause changes in the DNA. These changes are irreversible and…
Q: What is probe? Why it is used in the library screening ?
A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…
Q: What is single molecule sequencing in real time (SMRT) ?
A: DNA sequencing is a technique for determining the order of the four nucleotide bases found in DNA:…
Q: Why is NHEJ an error-prone mechanism?
A: DNA stands for deoxyribonucleic acid. It is the genetic material of the organisms that transfer from…
Q: Describe two different types of protein microarrays, and discuss their uses.
A: Genomics and proteomics are relatively new branches of genetics. Genomics is the study of all the…
How is the Bicinchoninic acid assay used in protein quantification?
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- Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what is the maximum amount of protein a sample could contain and still fall within a standard curve? Briefly explain your reasoning.
- What is an ONPG test? WHat is he principle behind this assay.What is the purpose of a standard curve for a colorimetric enzyme assay?Which of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions. For those that were not true in question 1, correct the statement(s).
- What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of the DNA fingerprinting?What does MEGA stand for and what is this assay used to determine? How to do perform the assay?What is meant by the term genetic testing? How do testing at theprotein level and testing at the DNA level differ? Describe fivedifferent techniques used in genetic testing.