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- How to isolate collagen from fish skin in the laboratory? Please write down each step in detail. Which chemicals are used and how much used. Why are they used, write them in detail. the main steps are as follows Washing and cutting of raw material Chemical pretreatment Extraction Precipitation Freeze-dryingWhat is the purpose of fixing a smear? Mark all that apply: To kill the bacteria so they aren't harmed by the staining method O To kill the bacteria to make the slide safer to handle O To attach the bacteria to the slide To break down the cell wall in order to make the cells accept stain To cause the cells to shrink and become distorted Microsoft Bing 11:30 AM do 87 F Sunny 9/14/2021What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle
- Why sometimes specimen may show organisms under the microscope but not appear in the culture media?Does the image above represent a pure culture? Yes O NoThis part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordant
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: O So that cells are not clumped and don't entrap stain creating erroneus results So that no contaminants are introduced onto the slide by being entrapped in clumps OSo that the cells are spread out enough that cell morphology can be discerned OSo that the cells are spread out enough that the arrangement can be observed O So that there are small groups of cells clumped together to make them visible Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021Microscopic samples can be stained. the stains are used to increase and improve resolution and contrast resolution and magnification resolution contrast O magnificationWith liquid media such as broth, is it possible to determine if the culture is pure using the naked eye only? no yes sometimes
- If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected. SubmitRequest AnswerBefore viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).On which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinose