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- QUESTION 7 SR proteins bind to splicing enhancers and activate splicing by recruiting spliceosome components. Mutations that affect SR proteins will have what kind of effect on gene expression? General transcription factors will be unable to bind to promoters. Splicing of the MRNA will not be activated. Ribosomes will not be able to bind to mRNA molecules in the cytoplasm. Polyadenylation at the 3' end will be blocked. RNA polymerase will be unable to terminate transcription. O0000Question 7C You cause a mutation in the bicoid gene in a Drosophila embryo. What effect will this have on this embryo? Will there be any other effects?QUESTION 11 Which of the following statements about RNA interference pathways and mechanisms is FALSE? O A Investigators can load RISC and/or RITS complexes with artificial, designed RNAS in order to specifically target and silence a gene of interest as a way to see what the effect of that gene is when its expression is silenced. O B. Only a small section of a small interfering RNA, called the 'seed' sequence, is actually used to identify nucleic acid targets that are to be silenced. OC. Eukaryotic RNA interference pathways do not regulate genes from the genome itself (i.e. endogenous genes), they only respond to external infection events such as invading viruses. The piRNA pathway processes small RNAS out of long noncoding RNA transcripts and is important for suppression of repetitive element movement in germ cells. OD. O E. The micro (n RNA pathway and the short interfering (si) pathways load small RNAS into RISC or RITS complexes.
- QUESTION 4 The following diagram represents double-stranded DNA that is part of the RNA-coding sequence of a transcription unit. A e C B TGT ACT GCA AGC AAC ACA TGA CGT TCG TTG The sequence of the RNA product of transcription is 5'-UGU ACU GCA AGC AAC-3'. Which strand is the non-template strand, the strand on the top or on the bottom? In the sequence above, which arrow is pointing to the 5' end of the top strand (a or b)?Question 4 "In this mutation, the AGC is transcribed into UAG. " O missense mutation O nonsense mutation O silent mutation O frameshift mutationQuestion 20 Which of the following is not true with respect to eukaryotic genes? a They contain introns which do not code for protein b They can exist as operons, with multiple genes under the control of a single promoter c They require transcription factors for initiation of transcription d Termination sites for transcription are not well defined
- Question 5 What is the role of TRF2 at the telomere? extends the 3' end of the G+T strand O packages DNA into heterchromatin O forms a platform for microtubule attachment O facilitates formation of the t-loop at the telomere and recruits RAP1AsapQUESTION 7 After you graduate, you are working at a laboratory that identifies a novel protein called Leprechaun. To determine in which organelles Leprechaun is found, you decide to express the Leprechaun gene in HeLa cells using a plasmid. Since you have generated an antibody that recognizes an epitope within the Leprechaun protein, which technique will you use to identify its localization within the HeLa cells? OA. SDS-PAGE O B. Immunocytochemistry O C. Western blot OD. Transmission electron microscopy (TEM) OE. Immunohistochemistry QUESTION 8 Which of the following statements regarding electron microscopy is TRUE? OA. White light is utilized to illuminate the sample for imaging in all types of electron microscopy. OB. Electrons pass through the object being examined in transmission electron microscopy (TEM). OC. In scanning electron microscopy (SEM) electrons, electrons bounce off the surface of the sample. OD. Electron microscopy can be used to view structures as small as 2 nm in…
- Question 26 Which of the following best describes the assembly process to initiate translation in prokaryotes? A small ribosome subunit associates with large subunit; mRNA associates next; IFs bring in first f-Met-tRNA; bound NTP hydrolyzed. B IF w/ bound NTP binds f-Met-tRNA; mRNA associates next; small subunit associates next; bound NTP hydrolyzed; large subunit associates. CIF binds f-Met-tRNA; small ribosome subunit associates next; mRNA associates next; then large subunit; bound NTP hydrolyzed. D small subunit associates with mRNA; IF w/ bound NTP brings in first f-Met-tRNA; IF then hydrolyses bound NTP; large subunit associatesquestion 26 What is true for CpG Islands: stretches of a few hundred base pairs of DNA where cytosines are unmethylated are not associated with genes are not found around the promoters are associated with silenced genesQuestion 14 The term "gene expression" refers to the O process by which genetic information is transformed nucleic acid information into a functional protein. the fact that each individual of a species has a unique set of genes. the fact that individuals of the same species have different phenotypes. flow of information from parent to offspring. Question 15 An operon consists of the coding sequences of a cluster of genes regulatory gene, coding sequences of a cluster of genes, an operator, and a promoter. a promoter, an operator and the coding region of a gene. a promoter and the coding region of a gene.