Q: What is molecular pharming? Compared with the production of proteins by bacteria, why might it be…
A: The production of foreign proteins by bacteria involves selection of the desired gene, incorporation…
Q: Why do critical instruments need to be sterile, whereas semicritical instruments need only be free…
A: Laboratory instrument is defined as a general term used for all kinds and types of instruments,…
Q: What are the different categories of cell viability assays? Describe one of them briefly. Define the…
A: Cell viability assays are the assays which are mainly used to determine or find out the number of…
Q: How do we know that during transduction bacterial cell-to- cell contact is not essential?
A: Transduction is a process of genetic recombination by which a foreign DNA is transferred into a…
Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…
A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…
Q: Define about immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assays ?
A: Introduction: A method of dissecting a substance to decide its structure or quality is known as an…
Q: On the four compounds testes for antibacterial activity using agar well diffusion assay, a. Which…
A: Antibacterial is an activity performed by any chemical or other compound through which it will the…
Q: Explain the steps of preparing a cell culture stock from a T25 flask.
A: Cell culture is the specialized process used in molecular biology to remove cells from plants and/or…
Q: How hand sanitizer can help us to keep our hand clean especially during COVID-19 pandemic?
A: There is no proof that liquor based hand sanitizers and other antimicrobial items are not good. They…
Q: Why are vent polymerase and Pfu more efficient than the Taq polymerase?
A: Polymerase is an enzyme that forms nucleic acids’ long chains. The RNA and DNA polymerase are used…
Q: What would you conclude if you examined a Kirby –Bauer assay and found individual colonies of the…
A: The Kirby - Bauer method is an universally accepted USFDA approved method commonly used in…
Q: What is the biological benefit(s) to the organism to have detachment induced cell death?
A: Anoikis is the programmed cell death that occurs in cells that detach from the surrounding…
Q: What approach can be used to enumerate the virus MNV-1 (murine norovirus) if you want to test a…
A: Murine norovirus (MNV-1): It is a virus that infects the mouse. This virus first came into…
Q: TGF-beta treatment cause cells to grow more or less in the soft-agar assay
A: TGF beta The transforming growth factor-beta is a cytokine a protein which is produced by all types…
Q: To maximize the number of mutations following UV irradiation, should you incubate the irradiated…
A: A mutation is a modification that happens in the deoxyribonucleic acid (DNA) sequence, either due to…
Q: Does endogenous contamination/component in crude extracted protein make interfere to transfer…
A: In. molecular biology, Western Blot is a technique for detection of specific proteins in a sample…
Q: Which of the environmental conditions below, must cancers cells adapt to for survival? High pH…
A: The cancer cells might adapt to these various environmental conditions: Telomerase getting too long…
Q: The table below shows the response of our ESKAPE safe relatives to 4 bacteria isolated from a master…
A: Antibiotics are the chemical substances that inhibit the growth of microbes.
Q: why didn't you choose to isolate some adult stem cells and what's the best cellular potency level…
A: Stem cell is a special human cell with unique ability to develop into many different cell types from…
Q: Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance…
A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…
Q: hat clinical trial phase is the most important?
A: A clinical trial testing new treatments are wholly divided into different stages , which are called…
Q: escribe which human cell machinery the SARS-CoV-2 uses in order to rep
A: Some useful terms : SARS ( Severe acute respiratory syndrome), CoV ( covid 19) ACE 2 (…
Q: A phage-infected bacterial culture was subjected to a series ofdilutions, and a plaque assay was…
A: Plaque assay is a method for the determination of the presence and number of bacteriophages.…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
A: Blue- white screening is used for the identification of recombinant bacteria.
Q: Will the results of an in vitro antibiotic sensitivity test always agree with the results of an in…
A: Invitro means the situation in which the experiments are conducted under the laboratory conditions.…
Q: If PBR323 is used instead of pUC18, is blue white screening applicable? Justify your answer.
A: Recombinant DNA is made from genetic recombination in the laboratory by joining genetic material…
Q: Describe how Blue/White screening reagent helps in screening the colonies of transformed cells.
A: The bacteria are a microscopic small organism. They are cultured in laboratories for many purposes.…
Q: Which type of genetic test analyzes the production of hexosaminidase?
A: correct option: 2 of the above Biochemical testing and Gene Testing Explanation: An absence of…
Q: The table below shows the response of our ESKAPE safe relatives to 4 bacteria isolated from a master…
A: Introduction :- Antibiotics are the drugs or the medicine's that are mostly referred in any kind of…
Q: Why won’t in vitro results necessarily ensure clinical success
A: INTRODUCTION Antibiotic Sensitivity test done by Kirby-Bauer method shows sensitivity for…
Q: in the plaque assay, what happened during the adsorption phase O the bacteria bound to the agar O…
A: The plaque assay is performed to determine the concentration of viruses in a solution. The quantity…
Q: In the shown disc diffusion assay, how many antibiotics have no antibacterial effect at all?: Select…
A: The Kirby-Bauer procedure also known as a disk diffusion test is performed to test the antibiotic…
Q: Is the GASP phenotype present? In LB? Un-Aged Cells Aged Cells LB culture Day 0 LB + NaCl culture LB…
A: Question - in the GASP phenotype ? in LB?
Q: transmission electron micrographs during viral infection cycles of both in vitro and in vivo…
A: Electron micrographs are obtained with the help of electron transmission microscope. It helps in the…
Q: How has Blue biotechnology been used to combat the COVID-19 pandemic?
A: Blue biotechnology : It is associated with aquaculture , coastal and marine biotech. It is…
Q: In the plaque assay, exactly what makes up a single plaque?
A: Viruses are non-cellular pathogenic particles. Viruses cannot survive on their own as they lack cell…
Q: explain or describe the principle of immunoturbidimetric C3 Assay.
A: Immunoturbidimetric: A technique that calculates the absorbance of light from an example which is…
Q: true or false Primary cells are isolated directly from tissues and have not been modified ex vivo…
A: Cell culture is the process of growing cells in-vitro or outside the body using suitable media and…
Q: Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as…
A: Intrinsic resistance can be described as "it is a natural resistance property, which is derived…
Q: STEP 1 YFP Trarsfect into ouilts STEP 2 Epitope-tagged protain (ancoded by eDNA) STEP 3 Lyse calls…
A: Protein is a polymer formed from amino acid joined by peptide bond. It is abundantly found in the…
Q: In ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the…
A: ELISPOT assay is an Enzyme-Linked Immune adsorbent Spot assay that is used for testing cellular…
Q: The table below shows the response of our ESKAPE safe relatives to 4 bacteria isolated from a master…
A: Introduction :- Antibiotic are the drugs or medicine which are mostly used against the bacterial…
Q: In an indirect ELISA what does the secondary antibody do (i.e, what purpose does it serve)?
A: ELISA or Enzyme Linked Immunosorbent Assay is a technique that quantifies and detects soluble…
Q: What is HIV? Discuss what an immunochromatographic assay is If a positive band appears in the test…
A: The Human Immunodeficiency Virus (HIV) is a virus that causes infection in humans. It is a virus…
Q: In what ways can you apply the principles of aseptic technique in your everyday life to maintain a…
A: Aseptic method means using procedures and procedures to prevent infection. It involves applying…
Q: Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block…
A: The enzyme linked immunosorbent assay (ELISA) is an analytical biochemistry assay which uses the…
Q: if our immume systems are capable of attacking the trypanosome, why do you think we might see the…
A: Parasites are creatures that live in or on a host and rely on it for food, housing, and…
Pathogenicity
Infection and Transmission
The infections are generated by the pathogenic organisms present in the environment. They maintain the capacity to invade a host body and establish colonies. A disease caused by such infectious agents is called a communicable disease or transmissible disease. These diseases spread through diverse means including blood, food, water, air, or vectors.
How did this experiment rule out the possibility that the R cells
simply used the dead S cells’ capsules to become pathogenic?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- With regard to the experiment described in Figure shown, Explain why an antibody was used to remove the bacteria thatwere not transformed. What would the results look like, in allfive cases, if the antibody/centrifugation step had not beenincluded in the experimental procedure?Why is the plate count method a determination of the number of viable cells?In Figure 5-5,a. Why do A− and B− cells, by themselves, not formcolonies on the plating medium?b. What genetic event do the purple colonies in themiddle plate represent?
- Does TGF-β treatment cause cells to grow more or less in the soft-agar assay? (a) More, (b) LessWhy is it so important to not vortex and/or pipette vigorously after you lyse the cells during the mini-prep? What would be the result if you did vortex?Following SDS-PAGE, what is an advantage of protein detection by immunoblotting (western blotting) over a non-specific gel-staining procedure? Under what circumstances would it be desirable to nonspecifically stain the gel, rather than immunoblotting?
- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?An inadequate priming or poor priming technique will cause a serious damage in patient. Would youplease explain it?If graft qnd corruption are diseases, how do you propose to ameliorate their ravages? If they are only symptoms, what is the disease and how do you propose to treat it?
- In a plaque assay, a phage has undergone _________ cycle when plaques are observed on the agar lawn post- incubation. What is the answer on the blank?A lab trainer instructed a new lab recruit to freeze down cells from a tissue culture flask to save for future use. After verifying that the flask of adherent cells was confluent, and contained enough cells to freeze down, the lab trainer left the recruit to go through the freezedown protocol. A few days later, the trainer thawed out the cryovials of cells that the recruit put away, and discovered that there were very few viable cells. Assuming that the lab trainer did everything correctly, answer the following: What are two possible mistakes made that explains why there are so few cells? Explain why those mistakes had the effect they did.Why must primary cell cultures be restarted every so often when preparing primary cell cultures to observe morphological changes caused by cells infected by a virus? Why are tumor cells preferred?