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- Why is DNA microarray an important tool and explain How is DNA microarray used in detecting cancer?Explain an example of a successful gene therapy trial. In your answer be sure to consider: a description of the disease condition that was treated, the mutation or disease gene affected, the therapeutic gene delivered, and the method of delivery use for the therapy.How can genetic testing help in cancer diagnosis? Briefly explain.
- In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account? Explain your answer.Discuss some potential issues regarding gene editing to correct genetic defects.
- Describe some methods used to deliver normal genes to cells for gene therapy treatments.A patient is diagnosed with lung cancer using imaging. To establish the best treatment option, doctors ask to profile the gene expression of the tumor, using a biopsy and microarray analysis. Describe the steps involved in the analysis in detail. The analysis reveals that a new gene therapy could be beneficial to the patient. Explain briefly how gene therapy could be used to cure cancer and detail how the treatment can be formulated for delivery to the patient. To prepare DNA for the gene therapy formulation, it is often important to measure the size of the molecule. Explain how this measurement can be done using agarose gel electrophoresis, taking care to detail the property of the DNA molecule at the basis of the technique. To generate large amounts of DNA to manufacture gene therapy payloads or to be able to see them on gel electrophoresis, specific sequences can be amplified by PCR. For a sequence of a 100 base pairs, calculate the number cycles of PCR required to generate 1 ng of…List three conditions for which gene therapy trials have been performed for, and suggest reasons for their success