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LO 64- Explain how Next gen sequencing is applied in different technologies
In which of the following scenario would it be best to use Next-gen sequencing? (select all that apply)
a-To separate fragments of DNA based on their size-
b-To insert a mutation at random in a gene
c-To idently a novel pathogen
d-To determine the
e- To insert methyl groups to cytosines in a DNA sequence
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- Part 3. Compare the Specificity of DNA-Cutting Tools The flexibility and specificity of CRISPR-Cas9 technology offer a large step forward for gene editing. The first DNA "scissors" were restriction enzymes, which cut DNA at predefined sequences, typically 4-8 base pairs long. For example, EcoRI, a restriction enzyme found in E. coli, will cut double- stranded DNA at every GAATTC sequence. If EcoRI were added to a sample that contained the entire human genome, it could cut at every GAATTC sequence. We can calculate the probability that a particular nucleotide sequence, such as GAATTC, will occur within a larger sequence. Table 1 below shows the calculated probabilities of finding sequences of particular lengths. These calculations are based on the assumption that DNA sequences are entirely random and that every nucleotide position has an equal probability of being A, T, C, or G. Use the table to answer the following questions. Table 1. Calculated probabilities of finding a specific…2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A circular plasmid molecule (12,000 total base pairs in size) was cut with a series of restriction enzymes and the digestions were size fractionated by agarose gel electrophoresis. Some digestions involved just one enzyme (single digest), some combinations of two enzymes (double digests), and one utilized all three enzymes (triple digest). Agarose gel electrophoresis of the digestions produced bands of the following sizes. Enzyme EcoRI Hindi!! Pstl EcoRI and Hindill EcoRI and Psti Hindill and Pstl EcoRI, Hindill, and Pst I Bands of the Agarose Gel (size in base pairs) 2300 and 9700 4000 and 8000 12,000 800, 1500, 2500, and 7200 2300, 3200, and 6500 4000 800, 1500, 2500, 3200, and 4000 I Draw a plasmid map showing the location of the restriction enzyme sites relative to each other for each map. Include all 7 maps in your answer.Genetically Modified Foods The creation of transgenic crop plants using recombinant DNA methods involves the transfer of just one gene or a small number of genes to the plants, in contrast to classical breeding methods in which hundreds or even thousands of genes are transferred at once. Explain why this is true. If fewer genes are transferred during the creation of transgenic crops, why are some people afraid that they are dangerous?
- Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.Choose the false statement. O Genetic engineering includes recombinant DNA techniques whereby DNA is hydrolyzed at specific sequences, pasted back together with ligase, and then inserted into cells of interest. O GMOS are organisms that, if eaten as a nutrient source, can genetically modify our DNA. OBacterial cells that have been transformed with the engineered plasmid can be selected for by utilizing antibiotics in the media and adding a drug resistance gene to the plasmid.Please answer this asap. Thanks, You have discovered a new plasmid RK21 in a unique bacterial community. As a first step towardunderstanding this plasmid, you digest the plasmid with three restriction enzymes: SspI, XhoI andSmaI. You run the digested plasmid DNA on an agarose gel, along with an uncut sample of theRK21 plasmid DNA as a control.Unfortunately you forget to load a DNA ladder, and obtain the following results. Assumecomplete digestion of all samples or all the digests worked completely
- BONUS: Why do RNA viruses such as the COVID coronavirus, influenza virus and HIV have much higher mutation rates than DNA viruses such as Herpes viruses? O DNA polymerases which copy viral RNA have much higher mutation rates than RNA polymerases which copy viral DNA O RNA polymerases which copy viral RNA have much higher mutation rates than DNA polymerases which copy viral DNA RNA viral 60S ribosomes make many ore mutations than DNA viral 40S ribosomes O RNA viral gyrases make more mistakes than DNA viral helicases4- Some/ one application(s) for DNA are/ is.... and they/ it start(s) from the mRNA. Therefore, the reverse transcriptase enzyme is required: a) DNA sequencing b) Electrophoresis c) Microarray d) All of the aboveOO HUAWEI Nova 2 Plus DUAL CAMERA The mammalian genome is compacted in a structure Answer: Now that we are all locked in our homes as a result of the coronavirus pandemic, what is this virus made out of and what technique they are using to check for the presence or absence of infection? Select one: O a. The corona virus is made out of RNA encapsulated in a protein capsid, it is detected by real time PCR O b. The corona virus is made out of DNA encapsulated in a protein capsid, it is detected by gel conventional PCR O C. The corona virus is made out of RNA encapsulated in a protein capsid, it is detected by gel electrophoresis d. The corona virus is made out of protein encapsulated in an RNA capsid, it is detected by real time PCR O e. The corona virus is made out of CDNA encapsulated in a protein capsid, it is detected by real time PCR NEXT PAGE
- OO HUAWEI Nova 2 Plus DUAL CAMERA estion To study the function of any gene of interest you would perform the loss and gain of function approaches by either deleting or re-expressing the gene of interest, which of the following can be used to determine and quantify the activity of the gene? red Oa. Microscopy d out of O b. Western blotting O C. PCR/OLA on O d. Gene knockdown O e. DNA hybridization stion Which one of the following is NOT correct regarding Bacterial Biosensors? O a. produced by all Pseudomonas species ed O b. Encoded by LacZ gene out of O c. Encoded by Lux genes O d. Chemical compounds used for the quantitative assessment of water pollution O e. In the presence of pollutants the bioluminescent decreases. stion CD4-Pseudomonas Exotoxin Fusion Protein is another biotechnology strategy for HIV therapeutic setting. This construct will target and kill: Select one: d O a. Any lymphocyte put of O b. HIV (virus only) O C. Non infected TH O d. Infected TH cells O e. Infected TH…Which viruses have potential for the highest mutation rates? Explain RNA viruses as RNA polymerases do not proofread thus make many mistakes/mutations when viral RNA is copied RNA viruses as DNA polymerases do not proofread thus make many mistakes/mutations when viral DNA is copied O DNA viruses as DNA polymerases do not proofread thus make many mistakes/mutations when viral RNA is copied O DNA viruses as DNA polymerases do not proofread thus make many mistakes/mutations when viral DNA is copiedGenetic Engineering Process (GEP) # 1: (What kind of process?) Picture A (Sequence #_ DNA introduced into bacterial cells Picture B (Sequence #, DNA ligase added, seals overhangs TTAA AATT AAT AATT TAA TTA TAA PATT PATT AATT recombinant DNA molecules Picture C (Sequence #. donor DNA vector vector and donor DNA digested (cleaved) with restriction enzyme AATT AATT 1477 AATT TTAA overhangs TTAA 1477 Picture D (Sequence #. AATT mixing recombinant DNA molecules replicate and cells divide
