Exercise 6 & 7:Acid-fast stain: what component/structure in the cell wall is stained?; be able to identify the stain techniqueand structure stained by sight (micrograph), give example genera (Nocardia and Mycobacterium), disease-causing species, and diseasesEndospore stain: what component/structure in the cell wall is stained?; be able to identify the stain techniqueand structure stained by sight (micrograph), know what endospores are and example genera w/ endospores(Bacillus and Clostridium);Capsule stain: be able to identify the stain technique and structure stained by sight (micrograph), and knowhow stain is performed (negative stain, why brief heat-fixation and followed by a positive stain)Flagella stain: be able to identify the stain technique and structure stained by sight (micrograph), identify thepurpose of the stain, differentiate flagellar arrangement: peritrichous, monotrichous, lophotrichous,amphitrichous, etc)

Staining is a process where different types of stains or dyes are used to color the part of the cell…

Answered: Exercise 6 & 7: Acid-fast stain: what…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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INSTRUCTION: EXPLAIN THOROUGHLY THE TWO THEORIES INVOLVED IN GRAM STAINING (CELL WALL AND LIPIDS THEORIES) IN RELATION TO THE TWO STATEMENTS BELOW. In THEORY OF CELL WALL, Gram-positive bacteria have heavy peptidoglycan, which helps decolorizer (alcohol) to dehydrate the gram-positive cell wall and traps the crystal violet complex inside the cell wall and maintains the purple color of crystal violet. In THEORY OF LIPIDS, Gram-negative bacteria have high lipid amount (10-15%) in the cell wall, which makes decolorizer (alcohol) easily to remove the crystal violet complex, and then colorless cell wall is stained with safranin and appears pink/red color.
QNO 2: (a) write a note on Microscope, its types and uses
Exercise 5: Gram-stain: know steps, stains/reagents, and colors of Gm- and Gm+ after each step as well as overall process Predict cell color of Gm - and/or Gm + cells at end of erroneous process Interpret micrographs for Gm reaction
Hello, tutor! Please provide the Preferred Specimen/Tissue Medium with brief explanation of the following types of microtomes - Rotary Microtome- Rocking Microtome- Base Sledge Microtome- Sliding Microtome- Freezing Microtome- Cryotome- Vibrating Microtome
answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:
Phase contrast microscopy - Human Cheek Cells a. b. What is the basic principle of image formation using this microscopy technique? What can be observed and concluded from the image of the specimen?
SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…
Please help using the image i attached below. There are 3 parts to this question. Describe how this is related to light scattering. Note the field is always dark in this type of microscope (and hence, called dark-field). Why is this? Explain how this type of microscope can be used to detect (but not resolve) flagella.
B. Write the functions of different parts of Light Microscope.
What's More Now that vou are familiarized with the parts and its uses of the microscope, let us check e understanding. Keep moving! Direction: Answer the following questions briefly. 1. What are the functions of the base and the arm of the microscope? 2. What is the function of a revolving nosepiece? 3. Which connects the eyepiece to the revolving nosepiece with the objectives? 4. Describe the function of the mirror. 5 What are parts of the microscope are being connected by the inclination joint? What I Have Learned Directions: Fill in the blank to make the sentences complete. Choose the correct words l- in the box. is a curved glass that bends light that passes through it. regulates the amount of light on the specimen. 1. 2. for viewing. 3. The glass slide is placed on the 4. The bottom of the microscope, used for support is The holds two or more obiective lenses and can be rotated to easily change powe
Exercise 1B – Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
Q 2: Solve the followings (a) The name "complex media" means that we do not know what is in it, but it is a poor media True False (b) Darkfield microscopy is a better choice to observe transparent organisms compared to conventional brightfield microscopy. True False (c) Acid-fast staining is sued to differentiate bacteria that have a rich lipid content in their cell wall, such as the waxy mycolic acid. These complex lipid structures prevent staining of these cells using common stains and staining procedures. In Acid-fast staining, these cell walls are first made permeable to the stain by heating. The following decolorization using acid-alcohol solution will not remove the first dye from acid-fast cells. True False (d) Acid-fast staining is an important diagnostic tool in the identification of Mycobacterium tuberculasis True False

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