Example. A new plant species was discovered. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and bradford assay was performed. The absorbance reading of the plant extract is 0.272. What is the protein concentration, in μg/L, of the plant extract? A bovine serum albumin (BSA) Stock solution with a concentration of 250 μg/mL was used and mixtures with the following compositions and absorbance reading were prepared in picture.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter20: Iv Medication And Titration Calculations
Section: Chapter Questions
Problem 1.5P
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Example. A new plant species was discovered. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and bradford assay was performed. The absorbance reading of the plant extract is 0.272. What is the protein concentration, in μg/L, of the plant extract? A bovine serum albumin (BSA) Stock solution with a concentration of 250 μg/mL was used and mixtures with the following compositions and absorbance reading were prepared in picture.

Volume of BSA Volume of water
(mL)
Absorbance
at 595 nm
Tube #
(mL)
1
0.00
2.00
0.000
0.20
1.80
0.112
3
0.40
1.60
0.225
4
0.60
1.40
0.318
0.80
1.20
0.432
1.00
1.00
0.551
Transcribed Image Text:Volume of BSA Volume of water (mL) Absorbance at 595 nm Tube # (mL) 1 0.00 2.00 0.000 0.20 1.80 0.112 3 0.40 1.60 0.225 4 0.60 1.40 0.318 0.80 1.20 0.432 1.00 1.00 0.551
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