Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data obtained during the first 4 minutes are linear. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose.
Q: 5′ - A U G C A A U A C A A U C C G G A A U G A - 3′ Give the primary structure of the protein that…
A: The translation is the process of synthesis of amino acids from the mRNA. During the translation…
Q: Hydroxylation of proline and lysine residues in collagen structure, leads to creating more Select…
A: Collagen is a structural protein. It is a long, inelastic, tripple-stranded helical structure.…
Q: What makes an enzyme stereospecific? Give one example of an enzyme that stereospecific and discuss
A: An enzyme is a protein (or RNA) that can act as a biological catalyst and speed up the rate of…
Q: Which of the following is incorrect about the G protein signaling pathways? a. The binding of the…
A: The guanine nucleotide-binding protein (G-protein) cell signaling pathway is involved in many…
Q: 1q}. 1. Why is cyanmethemoglobin method preferred than other methods? 2. In what…
A: Hi, thank you for posting the question on Bartleby. As per the guidelines we can answer only one…
Q: hoose the conditions when PDH is activated. a) acetyl CoA/CoA ratio is high b) NAD+/NADH ratio is…
A: PDH is a regulatory enzyme situated in inner mitrochondrial membrane which is comprise of three…
Q: Define a technique for separating hundreds to thousandsof proteins with high resolution ?
A: Introduction: The protein of protein purification involves the separation of the protein and…
Q: Which of the following base pairings is correct for RNA? A) U and T B) G and T C) C and U D) C and A…
A: Since there are multiple questions and they are not interlinked, as per our company guidelines only…
Q: What is the process that sustains the redox balance of glycolysis under aerobic conditions
A: A redox reaction is where oxidation and reduction occur simultaneously. In glycolysis, there is a…
Q: In glycolysis, pyruvate kinase produces what high energy product and what low energy product?
A: Glycolysis is a pathway in which the Glucose (C6) is broken down into a 3 carbon product i.e. 2…
Q: There are 20 primary amino acids that make up all proteins. But why those 20? Why Leucine? Arginine?…
A: The name amino acids indicates that it is a compound containing both amine group and a carboxylic…
Q: Define how to Predict the amino acid sequence of a putative polypeptideencoded by a cloned gene…
A: A section of DNA thought to be a gene is known as a putative gene. Although the precise function of…
Q: For each of the fatty acid characterizations, select from the response list the structure of the…
A: There are multiple questions given. We will check all the given options and determine which is the…
Q: The activity of an enzyme is studied with and without an inhibitor. Specifically, the initial rates…
A: With the following details provided you are first supposed to plot a graph between substrate…
Q: What mutation can be prevented when we exercise?
A: As our body grows, damages, and repairs with its progression so does our genetic material. The DNA…
Q: Consider the following properties of the protein components of a sample mixture as provided in the…
A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…
Q: Starting as a lipid in some holiday prime rib, trace the path that energy and biomass make as that…
A: It is well known to us all that both mass and energy are conserved in the universe. Hence all mass…
Q: Question Completion Status: A 2.0kb bacterial plasmid 'BS1030' is digested with the restriction…
A: Restriction enzymes cleave the DNA at palindromic sequences. Plasmids are circular DNA molecules.…
Q: Which of the following is incorrect about the collagen structure? The covalent cross-links between…
A: Collagen is a protein made up of three alpha helices, containing repeating units of Gly-X-Y (X or Y…
Q: What would the order of elution be (first to last to come out of a size-exclusion chromatography…
A: Size exclusion chromatography is a technique for separating molecules based on their size and shape.…
Q: Quest. 3- Attempt any one. @ write a detail note on citric acid cycle. 5 Discuss mechalis menten…
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: Which of the following is incorrect about the enzyme chymotrypsin? Oa. It catalyzes the addition of…
A: Chymotrypsin is a digestive enzyme component of pancreatic juice. The enzyme catalyzes the cleavage…
Q: Which of the following is incorrect? O a. Metabolic pathways involve mostly intermediates or…
A: Metabolic pathways are very important for a cell to provide energy.metabolic pathways might be…
Q: Which of the following is incorrect about the free energy change? O a. None; all the other choices…
A: Free energy or Gibb's free energy is the intrinsic energy of the system. It is a portion of the…
Q: Which of the following is/are true about the primary structure of a protein? It does not include…
A: As per the central dogma of molecular biology, DNA contains the code that is necessary for the…
Q: What if all the data above was the same EXCEPT with the change of G & X? Their new data is below.…
A: The DNA double helix is made of two linked strands that are wound in a helical shape. The two…
Q: Discuss the factors that affect oxygen binding in hemoglobin.
A: Hemoglobin is an oilgomeric, allosteric conjugated protein. It is composed of four polypeptide…
Q: Which of the following is incorrect? a. The ratio "catalyzed rate/uncatalyzed rate" is known as…
A: The Enzymes as biological catalysts. In the enzyme catalyzed reaction, the substrate is converted to…
Q: In the peptide Ser-Cys-Ala-Gly, the N-terminal end is glycine. O cystein. O serine. Oalanine.
A: A peptide is a chain of amino acids, in which the individual amino acids are linked together through…
Q: All of the following are mechanisms for regulating enzyme activity in the cell, except: a. Rate of…
A: Enzymes catalyses a reaction, they remain unchanged after the completion of reaction. They just…
Q: Answer for the number 6 question, Thank you. No need for long explanation.
A: If energy is released in a reaction, these reactions are called exergonic. If energy is consumed in…
Q: norget (ol noitesingtosTRID insistib (a) Highlight four significant reasons why porphyrin…
A: Introduction: Photothermal cancer therapy is mainly used for treating solid tumors. The treatment…
Q: You are saying that the inhibitor is competitive inhibitor. But according to data Vmax for reaction…
A: Enzyme kinetics can be calculated more accurate by using lb plot which can be constructed by…
Q: 1. a) What is meant by the term "buffer solution"?
A: A buffer solution is an aqueous solution that can be used to maintain physiological pH when a small…
Q: 1. Proteoglycans, such as heparan sulfate are defined as having sulfated glycosaminoglycans that are…
A: fggd
Q: Which of the following is incorrect about the conversion of pyruvate and NADH to lactate and NAD+? O…
A: If a cell lacks mitochondria or sudden need of energy arises due to rapid consumption of oxygen by…
Q: Hello! Can you please answer the four questions below directly? This is related to GLUTEN from WHEAT…
A: Answer for the following questions are :
Q: Which of the following is incorrect about glycogen synthesis? O a. The addition of glucose is…
A: Glycogen is the reserve food material synthesised from glucose and stored in liver. Synthesised of…
Q: Which of the following is incorrect? a. Amino acids may have one or more triplet codes or codons b.…
A: A codon is a sequence of triplet nucleotides (a set of three consecutive nucleotides) on DNA or RNA…
Q: Which of the following is incorrect about an enzyme-catalyzed reaction? a. Its progress can be…
A: Enzymes are biocatalysts that catalyze biochemical reactions in the cell. There are various enzymes…
Q: Match the following terms with the appropriate type of movement across a cell diffusion active…
A: Molecules are transported across the plasma membrane through mechanisms such as passive transport…
Q: Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at…
A: According to Michaelis-Menten Kinetics, when the rate or velocity of an enzyme catalyzed reaction…
Q: Which of the following pair of amino acids has polar but neutral side chains? cysteine and…
A: Since there are multiple questions and they are not interlinked, as per our company guidelines only…
Q: make your own timeline on biochemistry development up to 2022
A: Biochemistry is the study of biochemical reactions and biological process by applying the knowledge…
Q: at is nitrogen fixation
A: Answer for the following question is :
Q: Integral membrane proteins can exist as all of the following, except: a. A single helix Ob. A beta…
A: Integral membrane proteins contains both hydrophilic and hydrophobic domains. The hydrophobic domain…
Q: 1. Why is cyanmethemoglobin method preferred than other methods? 2. In what circumstances is the…
A: There are multiple questions given and not mentioned as to which has to be answered. So, I will…
Q: Read instruction and answer question
A: Thermophilic bacteria live at higher temperatures such as hot springs and hot thermal vents. The…
Q: Im guessing you not the same person that looked into my exercise at first. Does that mean the…
A: Michaelis–Menten rate equation relates the Vo (initial reaction rate), Vmax (maximum reaction rate),…
Q: Use a calculation based on the pH partition model to determine the likelihood that a weakly acidic…
A: According to the pH partition model, for a weakly acidic drug the Henderson-Hasselback equation is…
NO EXCEL! Please help me to understand to how calculate this exercise without using excel. I'm not allowed to use computer during exam, and I only have learned to calculate these type of exercises by using excel.
If you can write as much detail as possible I will be thankful.
Step by step
Solved in 4 steps with 3 images
Is it possible to determine Km and Vmax without using excel? Because we are not allowed to use computer during exam, so I dont understand how its done without using excel?
Ok, thanks for explaining in details. But I dont understand where you get the values for trend line equation? 0.93 and 0.8936?
- Activity, Enzyme Kinetics Biol 250, Spring 2022 The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S] (umol/L) V [(µmol/L) min1] 5 22 10 39 20 65 50 102 100 120 200 135 (a) Estimate Vmax and KM from a direct graph of v versus [S]. Do you find difficulties in getting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better? (c) Finally, try an Eadie-Hofstee plot of the same data. (d) If the total enzyme concentration was 1 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute? (e) Calculate kcat/KM for the enzyme reaction. Is this a fairly efficient enzyme?Question: Determine the Km and Vmax for this enzyme/substrate combination. [Substrate] (mM) V0 (mM/min) 0.25 0.183 0.50 0.356 1.00 0.665 2.50 1.45 5.00 2.35 What is the concentration of substrate necessary to achieve a turnover rate of 1.00 mM/min?AutoSave 301-Enzyme Kinetics and Inhibition, Part 3 - Compatibility Mode - Word Search ff steve M SM File Home Insert Draw Design Layout References Mailings Review View Help E Share O Comments prepared five different concentrations of substrate (sucrose), and five different concentrations of inhibitor C (plus the control, with zero mM of inhibitor C). The following Table lists the inhibitor C concentrations [I], substrate concentrations [S], and resulting enzyme velocities (V.) for all six of these experiments: [I] O mM O mM O mM O mM O mM [S] Vo 1/[S] 1/ Vo 0.1 mM 0.333333333333 mM per minute 0.2 mM 0.50 0.3 mM 0.60 0.4 mM 0.666666666667 0.5 mM 0.714285714286 0.1 mM 0.1 mM 0.166666666667 0.1 mM 0.2 mM 0.25 0.1 mM 0.3 mM 0.30 0.1 mM 0.4 mM 0.333333333333 0.1 mM 0.5 mM 0.357142857143 0.20 mM 0.1 mM 0.111111111111 0.20 mM 0.2 mM 0.166666666667 0.20 mM 0.3 mM 0.20 0.20 mM 0.4 mM 0.222222222222 0.20 mM 0.5 mM 0.238095238095 0.3 mM 0.1 mM 0.083333333333 0.3 mM 0.2 mM 0.125 0.3 mM 0.3 mM…
- Initial rate data for an enzyme that obeys Michaelis–Menten kinetics areshown in the following table. When the enzyme concentration is 3 nmolml-1, a Lineweaver–Burk plot of this data gives a line with a y-intercept of0.00426 (μmol-1 ml s). (a) Calculate kcat for the reaction.(b) Calculate KM for the enzyme.(c) When the reactions in part (b) are repeated in the presence of 12 μM ofan uncompetitive inhibitor, the y-intercept of the Lineweaver–Burk plotis 0.352 (μmol-1 ml s). Calculate K′I for this inhibitor.ACTIVITY 10.8.2 1. Draw a schematic diagram on glycogenolysis by showing the structural formula of its substrate. Show the enzyme required in each step 2. How is glycogenolysis being regulated?ACTIVITY 10.7.2 Complete the table below for the gluconeogenesis with their corresponding precursor molecule GLYCEROL Steps Structural Formula activator and formula of of the Enzyme, Structural Is the reaction coenzyme required the product reversible or irreversible? substrate
- ACTIVITY 10.4.2 Explain your answer briefly but concisely. What are the steps in the energy investment phase? 2. What are the steps in the energy-yielding phase? 3. What are the enzymes involved in an irreversible reaction? How are these enzymes being activated? What about inhibited? 4. Which step in the glycolytic pathway would ensure that glucose is fated for glycolysis? 5. During strenuous activity, the demand for ATP in muscle tissue is vastly increased. In rabbit leg muscle or turkey flight muscle, the ATP is produced almost exclusively by lactic acid fermentation. ATP is formed in the payoff phase of glycolysis by two reactions, promoted by phosphoglycerate kinase and pyruvate kinase. Suppose skeletal muscle were devoid of lactate dehydrogenase. Could it carry out the strenuous physical activity; that is, could it generate ATP at a high rate by glycolysis? Explain. 6. The transformation of glucose to lactate n myocytes releases only about 7% of the free energy released when…KINETIC CONSTANT No Na2HPO4 25mM Na2HPO4 50mM Na2HPO4 Vmax nmol p-NP. Min- 20.3252 14.30615 17.30104 Km mM -0.819106 -0.46495 -0.352941 1. What does this suggest about the structure of the active side of the enzyme?Case Study: Enzyme Kinetics Data for new statin drug (inhibits HMG CoA reductase): ● ● I Substrate (UM) 0.5 1.0 1.5 2.5 3.5 || Rate of reaction (mm/min) 23.5 32.2 36.9 41.8 44 What are the Kms of the uninhibited and inhibited reactions? Rate of reaction in presence of 5.0 nM statin Using the above date, create a double-reciprocal (Lineweaver Burk) plot. What type of inhibition is shown by the new statin? What are the Vmax of the uninhibited and inhibited reactions? 16.67 25.25 30.49 37.04 38.91
- Matching Question: Researchers characterized a new enzyme and determined its Km=200 uM. Moreover, they found that at (Etotad10 nM this enzyme reaches maximum reaction velocity Venasa5 is 1. What is the keat of this enzyme? 2. At which concentration of the substrate this enzyme reaches initial reaction velocity (Vo) of 500 nM/s (assuming (Etotal is the same 10 nMy? 3. What is the maximum reaction velocity (Vmax) of the reaction catalyzed by this enzyme at (Etotail4 nM? Make sure to choose the correct units of measure. Note that some of the items from the answer list should NOT be used. v kcat 1. 400 uM - (S] - - Vmax 2 100 uM 500 s 450 uM 250 s1 * 501 7. so00 s 1 UM's SuMis 10 200 uM 11. 10 uMis 12 25 s 13. 500 nM/sACTIVITY 10.7.1 Complete the table below for the gluconeogenesis with their corresponding precursor molecule AMINO ACIDS Structural Formula of the Is the reaction Steps Enzyme, activator and Structural formula of the product reversible or irreversible? coenzyme substrate requiredPROBLEMS 8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A and B. The following results were obtained at fixed total enzyme čoncentration: Substrate conc" Initial velocity (absorbance units per minute) (mmol l-) With 1 mmol I-IB Uninhibited With 1 mmol I-1À 50 20 0.684 1.08 0.653 1.01 0.649 0.476 0.374 0.311 10 1.43 0.468 1.02 0.363 0.296 3.3 0.798 2.5 2.0 0.657 0.549 0.250 Comment on these results.