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Enumerate and describe the signs of emulsion instability. At least 5-7
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- The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…Please answer Questions 5-3, 5-4, and 5-5 (GC Gas Chromatography) 5-2. A sample mixture of ethyl benzoate (bp 212 Celsius) and dodecane (bp 216.2 Celsius) is injected on two GC columns.Column A has DC710 silicone oil as the stationary phase, and column B uses polyethylene glycol as the stationaryphase. Which substance would be certain to elute first from column A and would the same material be expected toelute first from column B? Which column, A or B, would be expected to give the better separation of these two substances? 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography. These materials havesimilar boiling points. List several GC variables and conditions that would make it easier to separate thesesubstances by gas chromatography. 5-4. Capillary GC columns have better resolution than packed columns even though the enormous surface area provided by the packing material is absent in capillary columns. Why? 5-5. Preparative GC requires…7-21. Calculate the NBOD of 200 mg/L of a casein (C8H₁2O3N₂) dairy waste. Assume the following reactions: C8H₁2O3N₂ + 80₂ = 8CO₂ + 3H₂O + 2NH3 NO3 + H+ + H₂0 NH3 + 20₂ 7-22. Derive an expression for the final temperature T. of the mixture of
- (4) Below are depictions of a student's TLC plates in which fractions 1 - 10 from their column are spotted. Lane 1 2 3 4 5 67 8 9 10 (a) Which fractions contain pure fluorenone? Which contain pure ferrocene? (b) Which lanes, if any, represent mixed fractions? (c) Would the student incorporate lanes 6 and 7 into an Erlenmeyer flask of a combined fraction? Explain with 10 words or less.A compound Y is to be determined by UV-visible spectrophotometry. A calibration curve is constructed from standard solutions of Y with the following results: 0.20 ppm, A = 0.870; 0.40 ppm, A = 0.840; 0.60 ppm, A = 0.800; 0.80 ppm, A = 0.750; 1.00 ppm, A = 0.700; 1.50 ppm, A = 0.620; and 2.00 ppm, A = 0.530. a. What is the equation of the calibration curve for compound Y? b. What is the linearity of the calibration curve for compound Y?Pe Identity Molecu tr, min w, min T (oC) Chart ak ler area weight (cm2) 1 Air peak small Tm=0, small 58 50 2 CH31 141,9 6,60 0,55 100 13,0 3 C₂H5l 156,0 9,82 1,00 127 12,0 4 ISO.C3H7I 170,0 11,90 1,04 139 10,0 5 n.C3H7I 170,0 13,04 1,08 148 7,2 6 CH2l2 261,8 19,10 1,60 193 8,0 8. A mixture of five alkyl iodides is separated by gas chromatography using a column packed with crushed five brick cooled with silicone oil. The column is so heated that its temperature rises linearly during the separation. What is the resolution of peaks: a. 2 & 3 b. 4 & 5 C. 5 & 6
- h) i) j) Ph + НО OSI(CH3)3 Br PPH3 1) "BuLi /THF/rt / 1.5 h H CH3 homo /rt / 1 h 2) -Si(CH3)3 OCH3 TiCl4 CH₂Cl₂/-78°C / 15 min PPh3/1₂ (1 eq) imidazole / THE 0°C-it3-23 Complete this table for H,O: T, °C P, kPa v, m³/kg Phase description 140 0.05 550 Saturated liquid 125 750 500 0.1401) The ratio strength of a 10mL vial of neostigmine methylsulfate injection USP is 1:1500. Calculate the amount of neostigmine methylsulfate in milligrams contained in the 10mL vial. (Round to the whole number).
- A compound X is to be determined by UV-visible spectrophotometry. A calibration curve is constructed from standard solutions of X with the following results: 0.50 ppm, A = 0.24; 1.5 ppm, A = 0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 0.59; 4.5 ppm, A = 0.70. A solution of unknown X concentration had an absorbance of A = 0.50. Find the slope and in te rcept of the calibration curve, the standard error in Y, the concentration of the solution of unknown X concentrat ion, and the standard deviation in the concentration of X. Construct a plot of the calibration curve and determine the unknown concentra tion by hand from the plot. Compare it to that obtained from the regression line.Describe the primary and secondary criteria for having achieved V ̇O2 max.A compound X is to be determined by UV/Visible spectrophotometry. A calibration curve is constructed from standard solutions of X with the following results: 0.50 ppm, A =0.24; 1.5 ppm, A =0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 0.59; 4.5 ppm, A =0.70. Find the slope and intercept of the calibration curve, the standard error in y, the concentration of the solution of unknown X concentration and the standard deviation in the concentration of X. Construct a plot of the calibration curve and determine the unknown concentration by hand from the plot (only for the calibration curve, slope and y-intercept. Make an equation on how to solve for the concentration of unknown).