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During transfer of liquids using a micropipette:
Would you consider your pipettes accurate ? Why?
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- During transfer of liquids using a micropipette: What would it mean if the mean is very close to the expected value but your standard deviation is high ?For investigating the effect of pressure (height of a column of liquid), a 50-mL burette was used with a small length of rubber tubing fixed to the bottom on which a clamp could be fitted to start/stop the flow of water (Figure 1). Effluent was collected in a beaker and mean flow rate was calculated (mL/s), and a stopwatch was used to measure how long it took for 5 mL of liquid to flow out. For the subsequent sections of the practical, a 25-mL burette was used, and the top was connected via rubber tubing to a 5-liter reservoir placed on the shelving above the bench. The tubing at the base was connected to the different flow modules that comprised the different configurations of tubes to be tested. a. Interpret the graph. b. What does this relate to in the cardiovascular system, and is it something that can change? How? c. What would be the implications for our ability to control blood flow to different tissues, if this were the only control mechanism available? d. Based on the…During a transfer of liquids using a micropipette: what would you and/or your professor consider an acceptable standard deviation for your pipette?
- Micropipettes have a two-position plunger with friction stops. Depressing to the (first / second) stop measures the desired volume and is the one you should use to fill the tip. The ( first / second) stop is used to introduce an additional volume of air to blow out any solution remaining in the tip when you are emptying the tip.For investigating the effect of pressure (height of a column of liquid), a 50-mL burette wasused with a small length of rubber tubing fixed to the bottom on which a clamp could befitted to start/stop the flow of water (Figure 1). Effluent was collected in a beaker and meanflow rate was calculated (mL/s), and a stopwatch was used to measure how long it took for5 mL of liquid to flow out. For the subsequent sections of the practical, a 25-mL burette wasused, and the top was connected via rubber tubing to a 5-liter reservoir placed on theshelving above the bench. The tubing at the base was connected to the different flowmodules that comprised the different configurations of tubes to be tested. Figure 1: A. Theoretical set-up of the experiment. B. Flow rate data with increasing pressure a. Interpret the graph.b. What does this relate to in the cardiovascular system, and is it something that canchange? How?c. What would be the implications for our ability to control blood flow to different…Order: Decadron 12mg in NS 50 mL to infuse over 30mins
- 1 2 3 1 point Mike needs to pipette 25 uL of solution. Which pipette should he use to pipette the most accurate amount of solution? P1000 OP20 1 point You need to pipette a solution of 150 uL. What pipette will you use? What will you set this pipette to (i.e. what will the numbers read on the pipette?) P1000; 0150 2000 P10 P200 ▶⠀ ⠀ > > n P10; 015 1 point Which of the following are best practices for using a micropipette? Select all that apply. Never exceed the upper or lower limit of the pipette If only pipetting a little extra, use a pipette outside of the range limit Point the pipette tip upwards Use a new tip for different liquids When withdrawing liquids, always release the plunger slowly P200; 150 P20; 150Explain the reason for the following rules: a. Always hold a loaded micropipette in a vertical positione. Always release the plunger slowlyf. Always watch to see the solution enter the tipHow do I calculate beers law? For serial dilutions. I want to look the concentration values from these absorbance readings in order to make a graph of conc vs absorbance. (Assume the first tube has a 1 unit/ml concentration) 1)1.180 2)0.513 3)0.107 4)0.019 5)0.003
- How will you know if you are pipetting the correct volume of liquid? Give at least 3 indicatorsFill in the blank with the correct dilution for each tube in the series. (4 blanks, 4 tubes total)(e) Complete the following table: Number printed on the micropipette's end (its plunger) 20 20 200 1000 1000 Number showing in the micropipette's display window OTOHOSHOSHOOO 0 7 0 1 0 5 1 0 5 1 0 0 0 2 1 Volume that the micropipette is set to draw up (include units in your answer) 6) Before using a micropipette, you have to first place a 7) Shown below are the steps required to transfer fluid from one tube to another using a micropipette. Write numbers in the blanks to indicate the correct order in which the steps should be performed. Slowly release the micropipette's plunger. Place the micropipette tip into the tube containing fluid you wish to draw up. Push down on the micropipette plunger until it reaches the second stop. Place the micropipette tip into the tube that you wish to expel fluid into. Push down on the micropipette plunger until it reaches the first stop. 8) Suppose that you're getting ready to draw fluid into a micropipette. To do so, you push the micropipette's plunger…